公开(公告)号：US20240336949A1

公开(公告)日：2024-10-10

申请号：US18628860

申请日：2024-04-08

申请人： BIO-RAD EUROPE GMBH ,  BIO-RAD LABORATORIES, INC.

发明人： CHRISTOPHE QUIRING ,  CHRISTINE FAVIER ,  PATRICE SARFATI ,  JEAN FRANCOIS MOUSCADET ,  RONALD LEBOFSKY ,  REBECCA DIEVART

IPC分类号： C12Q1/06 ,  C12N9/26 ,  C12N9/34 ,  C12N9/38 ,  C12N9/40 ,  C12N9/42 ,  C12N9/48 ,  C12Q1/04

CPC分类号： C12Q1/06 ,  C12N9/2428 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/2474 ,  C12N9/485 ,  C12Q1/04 ,  C12Q1/045 ,  C12Y302/01003 ,  C12Y302/01022 ,  C12Y302/01035 ,  C12Y304/11

摘要： Methods, compositions, and kits are provided for rapidly analyzing microbial growth and/or number in a plurality of water-in-oil emulsion droplets.

公开(公告)号：US12102720B2

公开(公告)日：2024-10-01

申请号：US17556316

申请日：2021-12-20

申请人： Translate Bio, Inc.

发明人： Braydon Charles Guild ,  Michael Heartlein ,  Frank DeRosa ,  Jerry Chi Zhang

IPC分类号： A61K9/19 ,  A61K9/127 ,  A61K31/7088 ,  A61K38/45 ,  A61K38/46 ,  A61K38/47 ,  A61K38/50 ,  C07C323/25 ,  C07C323/27 ,  C07C323/44 ,  C07D233/64 ,  C07J41/00 ,  C07J43/00 ,  C12N15/88 ,  A61K9/51

CPC分类号： A61K9/19 ,  A61K9/1271 ,  A61K9/1272 ,  A61K31/7088 ,  A61K38/45 ,  A61K38/465 ,  A61K38/47 ,  A61K38/50 ,  C07C323/25 ,  C07C323/27 ,  C07C323/44 ,  C07D233/64 ,  C07J41/0055 ,  C07J43/003 ,  C12N15/88 ,  A61K9/5123 ,  C12Y201/03003 ,  C12Y203/01 ,  C12Y207/08015 ,  C12Y301/06 ,  C12Y302/01022 ,  C12Y302/01035 ,  C12Y302/01046 ,  C12Y302/01076 ,  C12Y305/03001

摘要： Disclosed herein are novel compounds, pharmaceutical compositions comprising such compounds and related methods of their use. The compounds described herein are useful, e.g., as liposomal delivery vehicles to facilitate the delivery of encapsulated polynucleotides to target cells and subsequent transfection of said target cells, and in certain embodiments are characterized as having one or more properties that afford such compounds advantages relative to other similarly classified lipids.

公开(公告)号：US20240240195A1

公开(公告)日：2024-07-18

申请号：US18584965

申请日：2024-02-22

申请人： Tropic Biosciences UK Limited

发明人： Eyal MAORI ,  Yaron GALANTY ,  Cristina PIGNOCCHI ,  Angela CHAPARRO GARCIA ,  Ofir MEIR

IPC分类号： C12N15/82 ,  A23F5/02 ,  C12N9/40

CPC分类号： C12N15/8243 ,  A23F5/02 ,  C12N9/2465 ,  C12N15/8213 ,  C12Y302/01022

摘要： A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding alpha-D-galactosidase. Also provided is a method of increasing extractability of solids from coffee beans. In addition there is provided a method of producing soluble coffec.

公开(公告)号：US20240218342A1

公开(公告)日：2024-07-04

申请号：US18556642

申请日：2022-04-25

申请人： Lai-Xi WANG ,  Xiao ZHANG

发明人： Lai-Xi WANG ,  Xiao ZHANG

IPC分类号： C12N9/40 ,  A61K38/00 ,  C07H17/00 ,  C12N9/24

CPC分类号： C12N9/2465 ,  C07H17/00 ,  C12N9/2402 ,  C12Y302/01022 ,  C12Y302/01096 ,  A61K38/00

摘要： The present disclosure provides compounds useful for enzymatic glycan remodeling of a glycoprotein. Also provided is a method for remodeling a glycoprotein using M6P-glycan oxazolines in a one-pot deglycosylation/transglycosylation process, which may enable site selective M6P-glycan remodeling of glycoproteins to obtain homogeneous products. The remodeled glycoprotein (such as a recombinant human acid α-glucosidase) may have enhanced affinity for the CI-MPR, increased uptake by a cell, and improved therapeutic efficacy compared to the original glycoprotein. A method of treating Pompe disease using a glycan remodeled lysosomal enzyme is also provided.

公开(公告)号：US20240115502A1

公开(公告)日：2024-04-11

申请号：US18274631

申请日：2022-01-26

申请人： CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC) ,  NANOMOL TECHNOLOGIES, S.L. ,  LEANBIO

发明人： Leonor VENTOSA RULL ,  Jaume VECIANA MIRÓ ,  Miriam ROYO EXPOSITO ,  Judit TOMSEN MELERO ,  Ibane ABASOLO OLAORTUA ,  Simón SCHWARTZ NAVARRO ,  Jose Luis CORCHERO NIETO ,  Daniel PULIDO MARTINEZ ,  Edgar CRISTÓBAL LECINA ,  Elisabet GONZÁLEZ MIRA ,  Santiago SALA VERGÉS ,  Alba CÓRDOBA INSENSÉ ,  Josep MERLO MAS ,  Andreu SOLDEVILA FÁBREGA ,  Albert FONT INGLÉS

IPC分类号： A61K9/127 ,  A61K38/47 ,  A61K47/26 ,  A61P3/06

CPC分类号： A61K9/1272 ,  A61K9/1277 ,  A61K38/47 ,  A61K47/26 ,  A61P3/06 ,  C12Y302/01022

摘要： The present invention refers to a liposome comprising: a) a phospholipid; b) cholesterol (chol); c) a conjugate comprising a cholesterol moiety, a polyethylene glycol (PEG) moiety and a peptide moiety comprising a RGD sequence, wherein the PEG moiety is covalently attached to the cholesterol moiety by one end via a bond of the type alkyl ether and is covalently attached to the peptide moiety comprising the RGD sequence by the other end: d) a non-lipid cationic surfactant present in a percentage of less than 30% mol in respect to the total mol of the components of the liposome a), b), c) and d); and e) alpha-galactosidase (GLA) enzyme present in a ratio of micrograms of GLA in respect to the total milligrams of the components of the liposome a), b), c) and d) of between and including 2 to 35. It also refers to a pharmaceutical composition that comprises it and to the liposome or the pharmaceutical composition for use as a medicament, in particular for use in the treatment of Fabry disease. It also refers to a process for the production of the liposome.

公开(公告)号：US20240035056A1

公开(公告)日：2024-02-01

申请号：US18274770

申请日：2021-05-06

申请人： NANJING FORESTRY UNIVERSITY

发明人： Caoxing HUANG ,  Qiang YONG ,  Yuheng TAO ,  Chenhuan LAI ,  Yong XU ,  Xin LI

IPC分类号： C12P19/04 ,  C12N9/40 ,  C12N9/24

CPC分类号： C12P19/04 ,  C12N9/2465 ,  C12Y302/01022 ,  C12N9/2491 ,  C12Y302/01025

摘要： A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and Trichoderma reesei (T. reesei) as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of β-mannanase and α-galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.

公开(公告)号：US20240025943A1

公开(公告)日：2024-01-25

申请号：US17769570

申请日：2020-10-16

申请人： eleva GmbH

发明人： Berengar JAHN ,  HOLGER NIEDERKRÜGER ,  JONAS KOCH ,  ANDREAS BUSCH ,  ANDREAS SCHAAF

IPC分类号： C07K1/14 ,  C12N9/40

CPC分类号： C07K1/145 ,  C12N9/2465 ,  C12Y302/01022

摘要： The invention concerns a method for detaching expressed material from the surface or from the apoplast of plant cells, wherein the plant cells are treated with a rotor-stator in a liquid medium, wherein the specific heat from the rotor-stator introduced by rotation of the rotor is a maximum of 3 kJ per kg of the liquid medium and per g/L dry weight of the plant cells and the specific heat capacity introduced into the medium is a maximum of 1.5 kJ per kg of the liquid medium per minute and per g/L dry weight of the plant cells.

公开(公告)号：US11751584B2

公开(公告)日：2023-09-12

申请号：US17340271

申请日：2021-06-07

申请人： Novozymes A/S

发明人： Lone Carstensen ,  Nikolaj Spodsberg ,  Morten Gjermansen ,  Jesper Salomon ,  Kristian Bertel Roemer M. Krogh ,  Eduardo Antonio Della Pia

IPC分类号： A61K38/00 ,  A23K20/189 ,  A23K20/174 ,  A23K20/22 ,  A23K20/24 ,  A23K20/20 ,  A23K40/10 ,  A23K10/30 ,  C12N9/40

CPC分类号： A23K20/189 ,  A23K10/30 ,  A23K20/174 ,  A23K20/22 ,  A23K20/24 ,  A23K20/30 ,  A23K40/10 ,  C12N9/2465 ,  C12Y302/01022

摘要： The present invention relates to methods of releasing galactose from legumes using polypeptides having alpha-galactosidase activity. The invention also relates to polypeptides having alpha-galactosidase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. The invention also relates to compositions comprising the polypeptides of the invention and the use of the polypeptides in animal feed.

公开(公告)号：US11690812B2

公开(公告)日：2023-07-04

申请号：US17190018

申请日：2021-03-02

申请人： Duke University ,  National University of Singapore

发明人： Dwight D. Koeberl ,  Paul M. Yen ,  Benjamin L. Farah

IPC分类号： A61K31/137 ,  A61K31/436 ,  A61K31/05 ,  A61K31/155 ,  A61K31/192 ,  A61K38/47 ,  A61K31/522 ,  A61K38/46 ,  A61K31/198 ,  A61K31/12 ,  A61K31/216 ,  A61K31/352 ,  A61K31/167 ,  A61P3/00 ,  A61K31/277 ,  A61K31/355 ,  A61K31/385 ,  A61K31/5415 ,  A61K31/55 ,  A61K31/575 ,  A61K31/7016 ,  A61K33/00

CPC分类号： A61K31/137 ,  A61K31/05 ,  A61K31/12 ,  A61K31/155 ,  A61K31/167 ,  A61K31/192 ,  A61K31/198 ,  A61K31/216 ,  A61K31/277 ,  A61K31/352 ,  A61K31/355 ,  A61K31/385 ,  A61K31/436 ,  A61K31/522 ,  A61K31/5415 ,  A61K31/55 ,  A61K31/575 ,  A61K31/7016 ,  A61K33/00 ,  A61K38/465 ,  A61K38/47 ,  A61P3/00 ,  C12Y301/04012 ,  C12Y302/0102 ,  C12Y302/01022 ,  C12Y302/01045 ,  C12Y302/01049 ,  C12Y302/01076 ,  A61K38/465 ,  A61K2300/00 ,  A61K38/47 ,  A61K2300/00

摘要： The present disclosure is directed to methods of treating a steatosis-associated disorder by administering a therapeutic agent selected from a lysosomal enzyme, an autophagy-inducing agent, or a combination thereof. Steatosis-associated disorders discussed herein include GSD Ia, GSD Ib, GSD Ic, NAFLD, and NASH. Other embodiments are directed to methods of reversing steatosis, modulating autophagy, inducing autophagy, and reversing glycogen storage.

公开(公告)号：US11685912B2

公开(公告)日：2023-06-27

申请号：US16978417

申请日：2019-03-05

申请人： TAKEDA PHARMACEUTICAL COMPANY LIMITED

发明人： Brian Dwyer ,  Bohong Zhang ,  Jun Hu ,  Muthuraman Meiyappan ,  Thomas Miller ,  Paul McLean ,  Clark Pan

IPC分类号： C12N9/40 ,  B01J20/286 ,  B01J20/289 ,  B01J20/32

CPC分类号： C12N9/2465 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3255 ,  C12Y302/01022

摘要： The invention relates to an affinity resin functionalized with small molecule inhibitors of glycoside-cleaving enzymes, e.g., α-galactosidase A (α-Gal A), glucocerebrosidase (GCB), β-galactosidase, and acid alpha-glucosidase (GAA), and a method for purifying glycoside-cleaving enzymes produced in a cell line using the small molecule inhibitor-functionalized affinity resin.