公开(公告)号：US5328843A

公开(公告)日：1994-07-12

申请号：US660875

申请日：1991-02-26

申请人： Jun Fukuda ,  Hideo Kawaguchi ,  Takejiro Ushiroda ,  Norio Shimizu ,  Kazuo Sato

发明人： Jun Fukuda ,  Hideo Kawaguchi ,  Takejiro Ushiroda ,  Norio Shimizu ,  Kazuo Sato

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12M3/00 ,  C12N5/07 ,  C12N5/071 ,  C12N5/10 ,  C12N5/06

CPC分类号： B01L3/0293 ,  B01L3/5027 ,  C12M33/00 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2400/0406

摘要： Apertures in a definite shape having a definite width and length are provided on one surface of a plate material having a definite thickness. One end of the apertures is connected with the other surface of the plate material and at the same time, is opened. Another end of the apertures is connected with the plate material on one surface and is also opened toward the other surface of a culture substrate. The plate material is closely contacted to one surface of the culture substrate for allocating nerve cells and observing growth of the neurites. Then, the cell suspension is supplied to the apertures. The cells in the suspension are allocated on the culture substrate in response to the apertures by centrifugation or spontaneous sedimentation. The plate material is then withdrawn.

摘要翻译： 在具有确定厚度的板材的一个表面上设置具有确定宽度和长度的确定形状的孔。 孔的一端与板材的另一面连接，同时打开。 孔的另一端在一个表面上与板材连接，并且朝向培养基底的另一表面打开。 板材与培养基质的一个表面紧密接触，用于分配神经细胞并观察神经突生长。 然后，将细胞悬浮液提供给孔。 悬浮液中的细胞通过离心或自发沉降响应于孔而分配在培养基底上。 然后取出板材。