Glucose isomerase mutants
    1.
    发明授权
    Glucose isomerase mutants 有权
    葡萄糖异构酶突变体

    公开(公告)号:US07919300B2

    公开(公告)日:2011-04-05

    申请号:US11597609

    申请日:2004-07-28

    IPC分类号: C12N9/92 C12P19/02 C07H21/04

    CPC分类号: C12N9/92

    摘要: This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.

    摘要翻译: 本发明描述了具有改进的催化活性和热稳定性的一系列重组的热酵母杆菌葡糖异构酶。 重组葡萄糖异构酶可用于直接生产含有55重量%或更高浓度果糖的果糖糖浆,或用于生产含有小于55重量%果糖的果糖糖浆。

    Isolated DNA encoding recombinant glucose isomerase
    2.
    发明授权
    Isolated DNA encoding recombinant glucose isomerase 有权
    分离的DNA编码重组葡萄糖异构酶

    公开(公告)号:US08067561B2

    公开(公告)日:2011-11-29

    申请号:US12723918

    申请日:2010-03-15

    CPC分类号: C12N9/92 C12P19/24

    摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

    摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gin),并携带至少一种其它的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。

    Glucose isomerase mutants, DNA thereof and use thereof
    3.
    发明授权
    Glucose isomerase mutants, DNA thereof and use thereof 有权
    葡萄糖异构酶突变体,其DNA及其用途

    公开(公告)号:US07704719B2

    公开(公告)日:2010-04-27

    申请号:US12093859

    申请日:2006-10-30

    CPC分类号: C12N9/92 C12P19/24

    摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.

    摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(wt)或更高浓度果糖的高果糖玉米糖浆。

    Methods of using isolated glucose isomerase
    4.
    发明授权
    Methods of using isolated glucose isomerase 有权
    使用分离的葡萄糖异构酶的方法

    公开(公告)号:US07923222B2

    公开(公告)日:2011-04-12

    申请号:US12723833

    申请日:2010-03-15

    CPC分类号: C12N9/92 C12P19/24

    摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

    摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gin),并携带至少一种其它的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。

    DNA comprising a DNA SEQ. of isolated glucose isomerase
    5.
    发明申请
    DNA comprising a DNA SEQ. of isolated glucose isomerase 有权
    DNA包含DNA SEQ。 的葡萄糖异构酶

    公开(公告)号:US20100228016A1

    公开(公告)日:2010-09-09

    申请号:US12723918

    申请日:2010-03-15

    IPC分类号: C07H21/04

    CPC分类号: C12N9/92 C12P19/24

    摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

    摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。

    Methods of using isolated glucose isomerase
    6.
    发明申请
    Methods of using isolated glucose isomerase 有权
    使用分离的葡萄糖异构酶的方法

    公开(公告)号:US20100227365A1

    公开(公告)日:2010-09-09

    申请号:US12723833

    申请日:2010-03-15

    IPC分类号: C12P19/24

    CPC分类号: C12N9/92 C12P19/24

    摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

    摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gin),并携带至少一种其它的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。

    Glucose Isomerase Mutants, Dna Thereof and Use Thereof
    7.
    发明申请
    Glucose Isomerase Mutants, Dna Thereof and Use Thereof 有权
    葡萄糖异构酶突变体及其用途

    公开(公告)号:US20080305529A1

    公开(公告)日:2008-12-11

    申请号:US12093859

    申请日:2006-10-30

    IPC分类号: C12P19/24 C12N9/92 C07H21/04

    CPC分类号: C12N9/92 C12P19/24

    摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.

    摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包含位置139的氨基酸变异,包括苯丙氨酸Phe),182位丙氨酸(Ala),第187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变的氨基酸 在位置87,位置217,位置260或位置276,并且当使用D-葡萄糖作为底物时,具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(wt)或更高浓度果糖的高果糖玉米糖浆。

    HYDRAULIC FRACTURING COMPOSITION, METHOD FOR MAKING AND USE OF SAME
    8.
    发明申请
    HYDRAULIC FRACTURING COMPOSITION, METHOD FOR MAKING AND USE OF SAME 有权
    液压破碎组合物,其制造和使用方法

    公开(公告)号:US20150096751A1

    公开(公告)日:2015-04-09

    申请号:US14570003

    申请日:2014-12-15

    IPC分类号: C09K8/80 E21B43/267

    摘要: A hydraulic fracturing composition includes: a superabsorbent polymer in an expanded state; a plurality of proppant particles disposed in the superabsorbent polymer; a well treatment agent, and a fluid to expand the superabsorbent polymer into the expanded state. A process for treating a well with well treatment agent includes disposing a hydraulic fracturing composition comprising the well treatment agent in a well. The well treatment agent can be a scale inhibitor, tracer, pH buffering agent, or a combination thereof.

    摘要翻译: 水力压裂组合物包括:膨胀状态的超吸收性聚合物; 设置在超吸收性聚合物中的多个支撑剂颗粒; 井处理剂和将超吸收性聚合物膨胀成膨胀状态的流体。 用井处理剂处理井的方法包括将包含井处理剂的水力压裂组合物置于井中。 该处理剂可以是阻垢剂,示踪剂,pH缓冲剂或其组合。