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公开(公告)号:US07919300B2
公开(公告)日:2011-04-05
申请号:US11597609
申请日:2004-07-28
申请人: Jun Wang , Rongzhao Fu , Dong Shen , Caike Jin , Zhangming Liu , Junming Chen
发明人: Jun Wang , Rongzhao Fu , Dong Shen , Caike Jin , Zhangming Liu , Junming Chen
CPC分类号: C12N9/92
摘要: This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.
摘要翻译: 本发明描述了具有改进的催化活性和热稳定性的一系列重组的热酵母杆菌葡糖异构酶。 重组葡萄糖异构酶可用于直接生产含有55重量%或更高浓度果糖的果糖糖浆,或用于生产含有小于55重量%果糖的果糖糖浆。
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公开(公告)号:US08067561B2
公开(公告)日:2011-11-29
申请号:US12723918
申请日:2010-03-15
申请人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gin),并携带至少一种其它的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。
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公开(公告)号:US07704719B2
公开(公告)日:2010-04-27
申请号:US12093859
申请日:2006-10-30
申请人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(wt)或更高浓度果糖的高果糖玉米糖浆。
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公开(公告)号:US07923222B2
公开(公告)日:2011-04-12
申请号:US12723833
申请日:2010-03-15
申请人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gin),并携带至少一种其它的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。
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公开(公告)号:US20100228016A1
公开(公告)日:2010-09-09
申请号:US12723918
申请日:2010-03-15
申请人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号: C07H21/04
摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。
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公开(公告)号:US20100227365A1
公开(公告)日:2010-09-09
申请号:US12723833
申请日:2010-03-15
申请人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号: C12P19/24
摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包括氨基酸变异,包括139位的苯丙氨酸(Phe),182位的丙氨酸(Ala),187位的丝氨酸(Ser)和299位的谷氨酰胺(Gin),并携带至少一种其它的突变氨基 在第87位,217位,260位或276位具有较高的酸性,当使用D-葡萄糖作为底物时,其具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(重量)或更高浓度果糖的高果糖玉米糖浆。
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公开(公告)号:US20080305529A1
公开(公告)日:2008-12-11
申请号:US12093859
申请日:2006-10-30
申请人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人: Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
摘要: This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
摘要翻译: 本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。 这些重组葡萄糖异构酶包含位置139的氨基酸变异,包括苯丙氨酸Phe),182位丙氨酸(Ala),第187位的丝氨酸(Ser)和299位的谷氨酰胺(Gln),并携带至少一种另外的突变的氨基酸 在位置87,位置217,位置260或位置276,并且当使用D-葡萄糖作为底物时,具有比野生型更高的催化活性。 这些重组葡萄糖异构酶可用于直接生产含有55%(wt)或更高浓度果糖的高果糖玉米糖浆。
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8.
公开(公告)号:US20150096751A1
公开(公告)日:2015-04-09
申请号:US14570003
申请日:2014-12-15
申请人: Dong Shen , Leiming Li , Jia Zhou , Hong Sun
发明人: Dong Shen , Leiming Li , Jia Zhou , Hong Sun
IPC分类号: C09K8/80 , E21B43/267
CPC分类号: C09K8/80 , C09K8/524 , C09K8/528 , C09K8/685 , C09K8/88 , C09K8/92 , C09K2208/20 , C09K2208/22 , C09K2208/26 , C09K2208/32 , E21B43/267
摘要: A hydraulic fracturing composition includes: a superabsorbent polymer in an expanded state; a plurality of proppant particles disposed in the superabsorbent polymer; a well treatment agent, and a fluid to expand the superabsorbent polymer into the expanded state. A process for treating a well with well treatment agent includes disposing a hydraulic fracturing composition comprising the well treatment agent in a well. The well treatment agent can be a scale inhibitor, tracer, pH buffering agent, or a combination thereof.
摘要翻译: 水力压裂组合物包括:膨胀状态的超吸收性聚合物; 设置在超吸收性聚合物中的多个支撑剂颗粒; 井处理剂和将超吸收性聚合物膨胀成膨胀状态的流体。 用井处理剂处理井的方法包括将包含井处理剂的水力压裂组合物置于井中。 该处理剂可以是阻垢剂,示踪剂,pH缓冲剂或其组合。
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9.
公开(公告)号:US08236734B1
公开(公告)日:2012-08-07
申请号:US13116819
申请日:2011-05-26
IPC分类号: E21B43/28
CPC分类号: C02F5/083 , C02F2103/10
摘要: The present invention relates to a method of using threshold scale inhibitors of the formula: wherein n is 2 or 3 and M is hydrogen or an alkali metal cation, for preventing calcium carbonate, iron carbonate, and calcium sulfate scale formation in oilwell brines containing dissolved iron.
摘要翻译: 本发明涉及一种使用下式的阈值抑制剂的方法:其中n为2或3,M为氢或碱金属阳离子,用于防止含有溶解的油井中的碳酸钙,碳酸铁和硫酸钙垢形成 铁。
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公开(公告)号:US09796914B2
公开(公告)日:2017-10-24
申请号:US14570003
申请日:2014-12-15
申请人: Dong Shen , Leiming Li , Jia Zhou , Hong Sun
发明人: Dong Shen , Leiming Li , Jia Zhou , Hong Sun
CPC分类号: C09K8/80 , C09K8/524 , C09K8/528 , C09K8/685 , C09K8/88 , C09K8/92 , C09K2208/20 , C09K2208/22 , C09K2208/26 , C09K2208/32 , E21B43/267
摘要: A hydraulic fracturing composition includes: a superabsorbent polymer in an expanded state; a plurality of proppant particles disposed in the superabsorbent polymer; a well treatment agent, and a fluid to expand the superabsorbent polymer into the expanded state. A process for treating a well with well treatment agent includes disposing a hydraulic fracturing composition comprising the well treatment agent in a well. The well treatment agent can be a scale inhibitor, tracer, pH buffering agent, or a combination thereof.
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