公开(公告)号：US06838556B2

公开(公告)日：2005-01-04

申请号：US09875453

申请日：2001-06-06

申请人： Jungsuh P. Kim ,  Douglas B. Starr ,  Albert W. Tam ,  Megan E. Laurance ,  Emil F. Michelotti ,  Mark D. Velligan ,  Derek R. Latour ,  Rita L. Thomas ,  Ana Kongpachith ,  Liana T. Sheppard ,  Moon Young Kim ,  Thomas W. Bruice

发明人： Jungsuh P. Kim ,  Douglas B. Starr ,  Albert W. Tam ,  Megan E. Laurance ,  Emil F. Michelotti ,  Mark D. Velligan ,  Derek R. Latour ,  Rita L. Thomas ,  Ana Kongpachith ,  Liana T. Sheppard ,  Moon Young Kim ,  Thomas W. Bruice

IPC分类号： C12N15/09 ,  C07K14/02 ,  C07K14/315 ,  C07K14/47 ,  C07K14/705 ,  C07K14/72 ,  C07K14/82 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/86 ,  C12Q1/68 ,  C12N15/11

CPC分类号： C07K14/005 ,  C07K14/315 ,  C07K14/4738 ,  C07K14/70578 ,  C07K14/72 ,  C07K14/82 ,  C12N9/86 ,  C12N2730/10122

摘要： This invention provides nucleic acid sequences, vectors and host cells comprising regulatory regions associated with various promoters including a cyclin D1 promoter, a CD40L promoter, three HBV promoters (core, pre-S1 and HBV-X), a vancomycin-resistant enterococci (VRE) promoter, an androgen receptor promoter, a Her2 promoter, and β-lactamase promoter. The invention further provides methods of regulating gene expression comprising the regulatory regions of such promoters.

公开(公告)号：US07189506B1

公开(公告)日：2007-03-13

申请号：US09518297

申请日：2000-03-03

申请人： Moon Young Lim ,  Cynthia A. Edwards ,  Kirk E. Fry ,  Thomas W. Bruice ,  Douglas B. Starr ,  Megan E. Laurance ,  Yan Kwok ,  Albert W. Tam

发明人： Moon Young Lim ,  Cynthia A. Edwards ,  Kirk E. Fry ,  Thomas W. Bruice ,  Douglas B. Starr ,  Megan E. Laurance ,  Yan Kwok ,  Albert W. Tam

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/90 ,  C07K2319/71 ,  C12N2800/40 ,  C12N2830/001 ,  C12N2830/15

摘要： The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.

摘要翻译： 本发明提供用于可调节基因表达的分子开关系统方法和组合物。 该系统包括具有与启动子可操作连接的转录调节蛋白的DNA应答序列的核酸构建体，DNA应答序列附近的化合物结合序列，启动子控制下的转基因; 和DNA结合化合物。 在一些情况下，分子交换系统还包括编码与第二启动子可操作地连接的转录调节蛋白的核酸序列。 本发明还提供了一种筛选化合物在分子开关系统中起作用并从而调节基因表达的方法。