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公开(公告)号：US20090155780A1
公开(公告)日：2009-06-18
申请号：US11957216
申请日：2007-12-14
Applicant: Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
Inventor： Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
CPC classification number: C12Q1/686 , C12Q1/6874 , C12Q2525/125 , C12Q2521/319 , C12Q2565/601 , C12Q2537/143
Abstract: Improved methods of genetic haplotyping and DNA sequencing and mapping, including methods for making amplified ssDNA, methods for allele determination, and a DNA barcoding strategy based on direct imaging of individual DNA molecules and localization of multiple sequence motifs or polymorphic sites on a single DNA molecule.
Abstract translation: 遗传单倍型和DNA测序和绘图的改进方法，包括制备扩增的ssDNA的方法，等位基因测定的方法和基于单个DNA分子的直接成像和多个序列基序或多态位点在单个DNA分子上的定位的DNA条形码策略 。
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公开(公告)号：US07829278B2
公开(公告)日：2010-11-09
申请号：US10976546
申请日：2004-10-28
Applicant: Paul R. Selvin , Matthew Gordon , Pui-Yan Kwok , Ming Xiao , Ting-Fung Chan
Inventor： Paul R. Selvin , Matthew Gordon , Pui-Yan Kwok , Ming Xiao , Ting-Fung Chan
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , C12Q1/6827
Abstract: A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.
Abstract translation: 使用这样一种方法对多核苷酸进行条形码化，由此将分离的单个多核苷酸固定在固相上并拉伸，使用靶特异性杂交探针对靶进行标记，并光学检测每个标记靶上的未扩增探针的单个标记。 确定标签的顺序以形成多核苷酸的条形码表示，其中表示靶标及其相对位置。
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公开(公告)号：US06440707B1
公开(公告)日：2002-08-27
申请号：US09708346
申请日：2000-11-07
Applicant: Pui-Yan Kwok , Xiangning Chen , Leanna Levine
Inventor： Pui-Yan Kwok , Xiangning Chen , Leanna Levine
IPC: C12P1934
CPC classification number: C12Q1/6816 , C12Q1/6827 , C12Q2527/125 , C12Q2522/101 , C12Q2563/107 , C12Q2561/119 , C12Q2535/131
Abstract: A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a oligonucleotide primer designed to hybridize to the DNA template adjacent to the polymorphic site in the presence of allelic dye-labeled dideoxyribonucleoside triphosphates and a modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number or samples.
Abstract translation: 描述了基于模板定向引物延伸和通过荧光偏振检测的DNA诊断的新方法。 在该方法中，将含有多态性位点的扩增的基因组DNA片段与设计为在等位基因染料标记的二脱氧核糖核苷三磷酸和修饰的Taq DNA聚合酶存在下与多态性位点相邻的DNA模板杂交的寡核苷酸引物温育。 该引物由对模板上存在的等位基因特异性的染料终止子延长。 在反应结束时，直接分析反应混合物中两个染料 - 终体化合物的荧光偏振而不进行分离或纯化。 这种均一的DNA诊断方法显示出高度敏感和特异性，适用于大量或样品的自动基因分型。
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公开(公告)号：US07771944B2
公开(公告)日：2010-08-10
申请号：US11957216
申请日：2007-12-14
Applicant: Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
Inventor： Ming Xiao , Pui-Yan Kwok , Paul Selvin , Mathew Gordon
CPC classification number: C12Q1/686 , C12Q1/6874 , C12Q2525/125 , C12Q2521/319 , C12Q2565/601 , C12Q2537/143
Abstract: Improved methods of genetic haplotyping and DNA sequencing and mapping, including methods for making amplified ssDNA, methods for allele determination, and a DNA barcoding strategy based on direct imaging of individual DNA molecules and localization of multiple sequence motifs or polymorphic sites on a single DNA molecule.
Abstract translation: 遗传单倍型和DNA测序和绘图的改进方法，包括制备扩增的ssDNA的方法，等位基因测定的方法和基于单个DNA分子的直接成像和多个序列基序或多态位点在单个DNA分子上的定位的DNA条形码策略 。
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公开(公告)号：US07189506B1
公开(公告)日：2007-03-13
申请号：US09518297
申请日：2000-03-03
Applicant: Moon Young Lim , Cynthia A. Edwards , Kirk E. Fry , Thomas W. Bruice , Douglas B. Starr , Megan E. Laurance , Yan Kwok , Albert W. Tam
Inventor： Moon Young Lim , Cynthia A. Edwards , Kirk E. Fry , Thomas W. Bruice , Douglas B. Starr , Megan E. Laurance , Yan Kwok , Albert W. Tam
CPC classification number: C12N15/90 , C07K2319/71 , C12N2800/40 , C12N2830/001 , C12N2830/15
Abstract: The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.
Abstract translation: 本发明提供用于可调节基因表达的分子开关系统方法和组合物。 该系统包括具有与启动子可操作连接的转录调节蛋白的DNA应答序列的核酸构建体，DNA应答序列附近的化合物结合序列，启动子控制下的转基因; 和DNA结合化合物。 在一些情况下，分子交换系统还包括编码与第二启动子可操作地连接的转录调节蛋白的核酸序列。 本发明还提供了一种筛选化合物在分子开关系统中起作用并从而调节基因表达的方法。
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Patent Agency Ranking
公开(公告)号：US06180408B2
公开(公告)日：2001-01-30
申请号：US09137826
申请日：1998-08-21
Applicant: Pui-Yan Kwok , Xiangning Chen , Leanna Levine
Inventor： Pui-Yan Kwok , Xiangning Chen , Leanna Levine
IPC: C12Q168
CPC classification number: C12Q1/6816 , C12Q1/6827 , C12Q2527/125 , C12Q2522/101 , C12Q2563/107 , C12Q2561/119 , C12Q2535/131
Abstract: A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a oligonucleotide primer designed to hybridize to the DNA template adjacent to the polymorphic site in the presence of allelic dye-labeled dideoxyribonucleoside triphosphates and a modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number or samples.
Abstract translation: 描述了基于模板定向引物延伸和通过荧光偏振检测的DNA诊断的新方法。 在该方法中，将含有多态性位点的扩增的基因组DNA片段与设计为在等位基因染料标记的二脱氧核糖核苷三磷酸和修饰的Taq DNA聚合酶存在下与多态性位点相邻的DNA模板杂交的寡核苷酸引物温育。 该引物由对模板上存在的等位基因特异性的染料终止子延长。 在反应结束时，直接分析反应混合物中两个染料 - 终体化合物的荧光偏振而不进行分离或纯化。 这种均一的DNA诊断方法显示出高度敏感和特异性，适用于大量或样品的自动基因分型。
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公开(公告)号：US20060094019A1
公开(公告)日：2006-05-04
申请号：US10976546
申请日：2004-10-28
Applicant: Paul Selvin , Matthew Gordon , Pui-Yan Kwok , Ming Xiao , Ting-Fung Chan
Inventor： Paul Selvin , Matthew Gordon , Pui-Yan Kwok , Ming Xiao , Ting-Fung Chan
CPC classification number: C12Q1/6858 , C12Q1/6827
Abstract: A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.
Abstract translation: 使用这样一种方法对多核苷酸进行条形码化，由此将分离的单个多核苷酸固定在固相上并拉伸，使用靶特异性杂交探针对靶进行标记，并光学检测每个标记靶上的未扩增探针的单个标记。 确定标签的顺序以形成多核苷酸的条形码表示，其中表示靶标及其相对位置。
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8.发明授权Synthesis of quinobenzoxazine analogues with topoisomerase II and quadruplex interactions for use as antineoplastic agents 失效合成具有拓扑异构酶II和四联体相互作用的喹诺酮类似物用作抗肿瘤剂公开(公告)号：US06528517B1
公开(公告)日：2003-03-04
申请号：US09245019
申请日：1999-02-04
Applicant: Laurence H. Hurley , Qingping Zeng , Yan Kwok , Jongsik Gam , Sean M. Kerwin
Inventor： Laurence H. Hurley , Qingping Zeng , Yan Kwok , Jongsik Gam , Sean M. Kerwin
IPC: A61K3144
CPC classification number: C07D498/06 , C07D498/16
Abstract: The present invention discloses a novel quinobenzoxazine self-assembly complex on DNA and on the topoisomerase II-DNA complex. The related model is used to design a new series of quinobenzoxazines, pyridobenzophenoxazines, pyrridonaphthophenoxazines, and other related compounds that may exhibit anticancer or antibiotic activity. The anticancer activity of these compounds is thought to operate via stabilization of the topoisomerase II-DNA complex and/or interaction with G-quadruplexes, while the antibiotic activity of these compounds derives from their ability to inhibit gyrase, the bacterial type II topoisomerase.
Abstract translation: 本发明公开了一种新的喹苯偶氮自组装DNA和拓扑异构酶II-DNA复合物的复合物。 相关模型用于设计一系列可能具有抗癌活性或抗生素活性的喹喔啉恶唑，吡啶并苯并恶嗪，吡哆苯并吩ox嗪等相关化合物。 认为这些化合物的抗癌活性通过拓扑异构酶II-DNA复合物的稳定化和/或与G-四链体相互作用而起作用，而这些化合物的抗生素活性来源于它们抑制细菌II型拓扑异构酶促旋酶的能力。
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9.发明授权Method for nucleic acid analysis using fluorescence resonance energy transfer 有权使用荧光共振能量转移进行核酸分析的方法公开(公告)号：US06177249B1
公开(公告)日：2001-01-23
申请号：US09295026
申请日：1999-04-20
Applicant: Pui-Yan Kwok , Xiangning Chen
Inventor： Pui-Yan Kwok , Xiangning Chen
IPC: C12Q168
CPC classification number: C12Q1/6818 , C12Q2600/156 , C12Q2565/1015 , C12Q2561/125 , C12Q2535/125 , C12Q2525/186 , C12Q2563/107
Abstract: A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.
Abstract translation: 公开了一种用于检测核酸中靶核苷酸或核苷酸序列的存在的方法。 该方法包括在核酸靶位点上形成用两个荧光团标记的寡核苷酸。 通过将单标记的双脱氧核苷三磷酸加成到单标记的多核苷酸中或通过连接两个单标记的多核苷酸来形成双重标记的寡核苷酸。 在变性时检测荧光共振能量转移指示靶的存在。 还提供套件。 该方法特别适用于基因分型。
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10.发明授权Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer 失效使用荧光共振能量转移进行核酸分析的方法和试剂盒
公开(公告)号：US5945283A
公开(公告)日：1999-08-31
申请号：US767979
申请日：1996-12-17
Applicant: Pui-Yan Kwok , Xiangning Chen
Inventor： Pui-Yan Kwok , Xiangning Chen
CPC classification number: C12Q1/6818 , C12Q2600/156
Abstract: A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.
Abstract translation: 公开了一种用于检测核酸中靶核苷酸或核苷酸序列的存在的方法。 该方法包括在核酸靶位点上形成用两个荧光团标记的寡核苷酸。 通过将单标记的双脱氧核苷三磷酸加成到单标记的多核苷酸中或通过连接两个单标记的多核苷酸来形成双重标记的寡核苷酸。 在变性时检测荧光共振能量转移指示靶的存在。 还提供套件。 该方法特别适用于基因分型。
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