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公开(公告)号：US20190211292A1
公开(公告)日：2019-07-11
申请号：US16331125
申请日：2017-09-12
IPC分类号： C12M1/00
摘要： The present disclosure is directed to bioreactor bag assemblies that can minimize the amount of additional connections/adaptations made to the bioreactor bag before the bioreactor bag can be used for cell cultivation, thereby reducing the risk of contamination. The bioreactor bag assemblies disclosed herein can include a pre-assembled waste bag connection and pre-assembled tubing arrangements so that the cell media and/or the cell source can be immediately welded to the pre-assembled tubing arrangements.
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公开(公告)号：US20190350978A1
公开(公告)日：2019-11-21
申请号：US16465140
申请日：2017-12-05
发明人： Pascal BEAUCHESNE , Semih U. TAREEN
IPC分类号： A61K35/17 , C12N5/0783 , C07K14/725 , C07K14/47
摘要： Provided are methods for genetically engineering cells, including cells for use in connection with genetic engineering. In some embodiments, the provided methods including transduction of cells by incubation with a retroviral vector particle, e.g. lentiviral vector, in which, prior to the incubation, the cells have not been incubated with an activating or stimulating agent, such as have not been incubated with anti-CD3/anti-CD28 antibodies and/or one or more recombinant cytokines. In some embodiments, such methods result in features related to shortening or improving the process for genetically engineering cells. Also provided are resulting cells, transduced with a recombinant or heterologous gene, such as one encoding a chimeric receptor such as a chimeric antigen receptor, or other recombinant antigen receptor such as a transgenic T cell receptor, and compositions thereof. In some embodiments, the provided cells and compositions can be used in methods of adoptive immunotherapy.
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公开(公告)号：US20230090176A1
公开(公告)日：2023-03-23
申请号：US17850875
申请日：2022-06-27
IPC分类号： A61K35/17 , B01D15/38 , C12N5/10 , C12N5/0783 , C12M1/00
摘要： The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods arc capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and/or resources and/or reduced handling compared with other methods.
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公开(公告)号：US20210207080A1
公开(公告)日：2021-07-08
申请号：US16770052
申请日：2018-12-07
发明人： Pascal BEAUCHESNE , Kien KHUU-DUONG , Ivie AIFUWA , Calvin CHAN
IPC分类号： C12N5/00 , C12N5/0783
摘要： Provided herein is a serum-free media for culturing, such as cultivating, preparing and/or producing cells, such as immune cells, such as genetically engineered cells. Also provided is a liquid basal media and frozen supplements that can be used to produce serum-free media. The provided embodiments include methods for producing serum-free media and methods for culturing cells, such as activating, transducing, cultivating or expanded cells, in the presence of serum-free media.
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公开(公告)号：US20190264153A1
公开(公告)日：2019-08-29
申请号：US16319799
申请日：2017-07-28
摘要： The present disclosure is directed to bioreactor consoles that provide a stable structure with built-in bioreactor component management. The bioreactor consoles disclosed herein can include one or more vertical platforms with notches and/or a recessed platform that can provide the desirable flexibility to easily connect and disconnect various tubes, cables, and components of the bioreactor system as well as organize which tubes and cables connect to which component of a bioreactor on the vertical platforms.
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6.发明公开公开(公告)号：US20230190814A1
公开(公告)日：2023-06-22
申请号：US18166447
申请日：2023-02-08
IPC分类号： A61K35/17 , C12N5/10 , B01D15/38 , C12M1/00 , C12N5/0783
CPC分类号： A61K35/17 , C12N5/10 , B01D15/3804 , C12M47/04 , C12N5/0636 , G01N2333/70514 , A61K2035/124
摘要： The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and/or resources and/or reduced handling compared with other methods.
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公开(公告)号：US20220228101A1
公开(公告)日：2022-07-21
申请号：US17617257
申请日：2020-06-05
发明人： Ivie AIFUWA , Pascal BEAUCHESNE , Kien KHUU-DUONG , Kohana LEUBA
摘要： An automated method of T cell scale down processing. The method including: activating T cells by automatically contacting the T cells with one or more activation reagents; transducing the T cells by automatically contacting the T cells with a recombinant viral vector; automatically inoculating T cells; automatically expanding the T cells; optionally, automatically debeading the T cells; and automatically harvesting the T cells. A system for an automated method of T cell scale down processing.
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8.发明申请METHODS FOR ISOLATING, CULTURING, AND GENETICALLY ENGINEERING IMMUNE CELL POPULATIONS FOR ADOPTIVE THERAPY 审中-公开用于分离，培养和遗传工程的免疫细胞群体用于通过治疗的方法公开(公告)号：US20170037369A1
公开(公告)日：2017-02-09
申请号：US15305337
申请日：2015-04-23
IPC分类号： C12N5/0783 , G01N33/569 , A61K35/17 , C12M1/00
摘要： The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and/or resources and/or reduced handling compared with other methods.
摘要翻译： 本公开在一些方面涉及用于制备用于基因工程和细胞治疗的细胞和组合物的方法，细胞和组合物。 在一些实施方案中提供了流线型的细胞制备方法，例如用于细胞和细胞群体的分离，加工，孵育和遗传工程。 还提供了通过其使用的方法和方法制备的细胞和组合物。 细胞可以包括免疫细胞，例如T细胞，并且通常包括多个分离的T细胞群体或类型。 在一些方面，与其他方法相比，该方法能够使用更少的步骤和/或资源和/或减少的处理来制备用于过继治疗的多个不同细胞群体。
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