公开(公告)号：US20160177265A1

公开(公告)日：2016-06-23

申请号：US14899828

申请日：2014-06-27

Applicant: KEIO UNIVERSITY ,  KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY

Inventor： Yumiko Matsubara ,  Takeru Zama ,  Yasuo Ikeda ,  Yukako Uruga ,  Toshio Suda ,  Sahoko Matsuoka

IPC: C12N5/078

CPC classification number: C12N5/0644 ,  C07K14/61 ,  C12N2500/24 ,  C12N2501/145 ,  C12N2506/13 ,  C12N2506/1384 ,  C12N2511/00

Abstract: Provided is a megakaryocyte and/or platelet production method, enabling to produce a megakaryocyte and/or platelet from mesenchymal cells such as preadipocytes in a relatively short period of time, simply, in a large amount and at lower cost or more efficiently in vitro and a method for producing TPO simply and in a larger amount. A first invention is a method for producing a megakaryocyte and/or platelet, comprising culturing a mesenchymal cell in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter and collecting megakaryocytes and/or platelets from a culture. A second invention is a method for producing thrombopoietin, comprising culturing a mesenchymal cell or mesenchymal cell-derived megakaryocyte in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter and collecting thrombopoietin from a culture. A third invention is a method for producing thrombopoietin, comprising culturing a preadipocyte in a preadipocyte culturing basic medium containing dexamethasone, 3-isobutyl-1-methylxanthine and insulin and collecting thrombopoietin from a culture.

Abstract translation: 提供了巨核细胞和/或血小板制备方法，其能够在相对较短的时间内简单地以大量的成本或更低的成本或更有效的体外从间充质细胞如前脂肪细胞产生巨核细胞和/或血小板，以及 一种简单且大量生产TPO的方法。 第一发明是一种生产巨核细胞和/或血小板的方法，包括在含有铁离子和铁转运蛋白的间充质细胞培养基础培养基中培养间充质细胞，并从培养物中收集巨核细胞和/或血小板。 第二个发明是一种生产血小板生成素的方法，包括在含有铁离子和铁转运蛋白的间充质细胞培养基础培养基中培养间充质细胞或间充质细胞来源的巨核细胞，并从培养物中收集血小板生成素。 第三发明是一种生产血小板生成素的方法，包括在含有地塞米松，3-异丁基-1-甲基黄嘌呤和胰岛素的前脂肪细胞培养基础培养基中培养前脂肪细胞并从培养物中收集血小板生成素。

公开(公告)号：US20240415893A1

公开(公告)日：2024-12-19

申请号：US18703653

申请日：2022-11-07

Applicant: Keio University

Inventor： Yoshitsugu Fukuda ,  Yasuo Niki ,  Masaya Nakamura ,  Yumiko Matsubara ,  Yukako Uruga

IPC: A61K35/28 ,  A61P19/02 ,  C12N5/0775

Abstract: An object of the present invention is to provide, for example, a therapeutic agent using a specific mesenchymal stem cell line derived from an adipose tissue (ASCL), the therapeutic agent (i) stably having an excellent therapeutic effect on one or more diseases selected from knee osteoarthritis, traumatic cartilage defect, and osteochondritis dissecans, (ii) loading less physical burden on a patient, (iii) requiring no restriction on patient's hospital visit management, and (iv) having no restriction on the number of treatments. A feature of the present invention is to use a mesenchymal stem cell line derived from an adipose tissue, the mesenchymal stem cell line being produced by a production method comprising: (A) inducing differentiation of one or more cells selected from a stromal vascular fraction of a vertebrate animal adipose tissue comprising a mesenchymal stem cell, an adipose progenitor cell, and a stromal cell into a mature adipocyte; and (B) inducing dedifferentiation of the mature adipocyte obtained in step (A) to obtain a mesenchymal cell line derived from the vertebrate animal adipose tissue.

公开(公告)号：US11712451B2

公开(公告)日：2023-08-01

申请号：US16162546

申请日：2018-10-17

Applicant: KEIO UNIVERSITY

Inventor： Yumiko Matsubara ,  Yasuo Ikeda ,  Keiichi Tozawa ,  Yukako Uruga ,  Masaki Yazawa ,  Taisuke Mori ,  Kazuo Kishi

IPC: A61K35/19 ,  A61P17/02

CPC classification number: A61K35/19 ,  A61P17/02

Abstract: An object of the present invention is to provide, for example, a more practical wound healing accelerator that more effectively accelerates wound healing. More specifically, a feature of the present invention is to provide, for example, a more practical wound healing accelerator that is easily obtained in a larger amount than that of peripheral blood platelet and has a better wound healing effect than that of peripheral blood platelet. The present invention employs a platelet-like cell population coexpressing one or more platelet surface markers and one or more mesenchymal cell surface markers. A wound healing accelerator containing the platelet-like cell population is a more practical wound healing accelerator that more effectively accelerates wound healing. The platelet-like cell population is easily obtained in a larger amount than that of peripheral blood platelet and has a better wound healing effect than that of peripheral blood platelet.