公开(公告)号：US3218565A

公开(公告)日：1965-11-16

申请号：US13625261

申请日：1961-09-06

Applicant: KIMISUKE SHIRAE ,  TAMOTSU KOBAYASHI ,  ISAO ABE

Inventor： KIMISUKE SHIRAE ,  TAMOTSU KOBAYASHI ,  ISAO ABE

IPC: G01D5/12 ,  G01D5/18 ,  G01K7/24 ,  G01L1/18 ,  G06G7/162

CPC classification number: G06G7/162 ,  G01D5/12 ,  G01D5/18 ,  G01K7/24 ,  G01L1/18

公开(公告)号：US20110262962A1

公开(公告)日：2011-10-27

申请号：US13087612

申请日：2011-04-15

Applicant: ISAO ABE ,  NOBUHIRO HIRATSUKA ,  SONOKO TAKEUCHI ,  SHUNICHI SUZUKI ,  KENZO YOKOZEKI

Inventor： ISAO ABE ,  NOBUHIRO HIRATSUKA ,  SONOKO TAKEUCHI ,  SHUNICHI SUZUKI ,  KENZO YOKOZEKI

IPC: C12P21/02

CPC classification number: C12P21/02 ,  C07K5/0613 ,  C12N9/1025 ,  C12Y603/02

Abstract: The present invention provides a method for producing a peptide, comprising culturing a transformant introduced with an expression vector to prepare a culture, and mixing the culture with a carboxy component and an amine component to form the peptide. The expression vector comprises a polynucleotide encoding a protein: (A) having selected deletions in the amino acid sequence of SEQ ID NO:2, (B) having a mutation of one or several amino acid residues in any protein selected from said group (A); (C) having 70% or more amino acid sequence identity to any protein selected from said group (A), (D) encoded by a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to a polynucleotide encoding any protein selected from said group (A), and (E) encoded by a polynucleotide having 70% or more nucleotide sequence identity to the polynucleotide encoding any protein selected from the group (A).

Abstract translation: 本发明提供一种生产肽的方法，包括培养引入了表达载体的转化体以制备培养物，并将培养物与羧基组分和胺组分混合以形成肽。 表达载体包含编码蛋白质的多核苷酸：（A）在SEQ ID NO：2的氨基酸序列中具有选择性缺失的蛋白质，（B）在选自所述组（A）的任何蛋白质中具有一个或几个氨基酸残基的突变 ）; （C）与选自由在严格条件下杂交的多核苷酸编码的所述组（A），（D）中选择的任何蛋白质具有70％或更多氨基酸序列同一性的多核苷酸，所述多核苷酸由与编码任何 （A）中选择的蛋白质的蛋白质和（E）编码的多核苷酸，所述多核苷酸与编码选自（A）组的任何蛋白质的多核苷酸具有70％以上的核苷酸序列同一性。

公开(公告)号：US20100279343A1

公开(公告)日：2010-11-04

申请号：US12749966

申请日：2010-03-30

Applicant: KENZO YOKOZEKI ,  AYAKO OHNO ,  SEIICHI HARA ,  ISAO ABE

Inventor： KENZO YOKOZEKI ,  AYAKO OHNO ,  SEIICHI HARA ,  ISAO ABE

IPC: C12P21/06

CPC classification number: C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

Abstract: A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-αβ-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-αβ-diester through a peptide bond.

Abstract translation: 通过从L-天冬氨酸-α和bgr-二酯和L-苯丙氨酸形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α和bgr-二酯的α-酯位点的酶或酶的物质。