公开(公告)号：US20160115491A1

公开(公告)日：2016-04-28

申请号：US14637355

申请日：2015-03-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Youngsoon UM ,  Yunje KIM ,  Gyeong Taek GONG ,  Jung Seok LEE

IPC: C12N15/77 ,  C12N15/72

CPC classification number: C12N15/77 ,  C12N15/72

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。

公开(公告)号：US20160002677A1

公开(公告)日：2016-01-07

申请号：US14494930

申请日：2014-09-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jung Seok LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Byoung Koun MIN ,  Jeong-Myeong HA ,  Yunje KIM ,  Sang Jun SIM

IPC: C12P7/46 ,  C12N15/77

CPC classification number: C12P7/46 ,  C12N15/77

Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

Abstract translation: 本公开涉及使用在二氧化碳作为直接碳源而不将其转化为葡萄糖而生长的微藻中积累的淀粉生产琥珀酸的菌株，以及使用该淀粉生产琥珀酸盐的方法。 本公开提供了从二氧化碳生产琥珀酸盐的菌株，其选自谷氨酸棒杆菌BL-1-pBlAmyS（KCTC 12585BP）和谷氨酸棒杆菌BL-1-pSbAmyA（KCTC 12587BP）。 本公开还提供了一种从二氧化碳生产琥珀酸的方法，包括通过在含淀粉培养基中从二氧化碳接种产生琥珀酸的菌株来发酵淀粉。