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    EXPRESSION VECTOR FOR CYANOBACTERIA
    3.
    发明申请
    EXPRESSION VECTOR FOR CYANOBACTERIA 有权
    CYANOBACTERIA表达载体

    公开(公告)号：US20160168580A1

    公开(公告)日：2016-06-16

    申请号：US14860373

    申请日：2015-09-21

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Han Min WOO ,  Jun Won CHWA ,  Youngsoon UM ,  Gyeong Taek GONG ,  Yunje KIM

    IPC: C12N15/74

    CPC classification number: C12N15/74

    Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

    Abstract translation: 本公开公开了可以用于蓝细菌和大肠杆菌的载体，其包含顺序地作为复制起点的pUC复制起点; 壮观霉素抗性基因作为选择标记; 和选自trc启动子，tetA启动子或修饰的tetA启动子，BAD启动子和cbbL启动子的启动子。 可以使用用载体转化的宿主细胞有效地产生工业上有用的物质。 此外，载体可以用于通过简单组合插入各种靶基因，结果可以有效地制备各种载体。

    METHOD FOR ISOLATING HIGHLY FURFURAL RESISTANT STRAIN AND STRAIN ISOLATED BY THE METHOD
    4.
    发明申请
    METHOD FOR ISOLATING HIGHLY FURFURAL RESISTANT STRAIN AND STRAIN ISOLATED BY THE METHOD 审中-公开
    用于分离方法分离高抗痘病毒株和菌株的方法

    公开(公告)号：US20160152942A1

    公开(公告)日：2016-06-02

    申请号：US14679622

    申请日：2015-04-06

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Han Min WOO ,  Youngsoon UM ,  Gyeong Taek GONG ,  Dong Jin SUH ,  Yunje KIM

    IPC: C12N1/20

    CPC classification number: C12N1/20 ,  C12R1/01

    Abstract: A method for isolating a highly furfural-resistant strain is disclosed. The method includes (A) mixing soil with an isotonic solution and collecting the soil supernatant from the mixture, (B) diluting the soil supernatant and spreading the dilution on a medium supplemented with carboxymethyl cellulose as a nutrient source, and (C) growing a desired strain by culture in the medium spread with the diluted soil supernatant and isolating the strain. Also disclosed is a strain isolated by the method. The strain is Enterobacter cloacae strain GGT036 [Accession No. KCTC 12672BP].

    Abstract translation: 公开了一种分离高糠醛抗性菌株的方法。 该方法包括（A）将土壤与等渗溶液混合并从混合物中收集土壤上清液，（B）稀释土壤上清液并将稀释液铺展在补充有羧甲基纤维素作为营养源的培养基上，（C） 通过在稀释的土壤上清液中培养的培养物中所需的菌株并分离菌株。 还公开了通过该方法分离的菌株。 菌株为阴沟肠杆菌菌株GGT036 [保藏号KCTC 12672BP]。

    SHUTTLE VECTOR FOR CORYNEBACTERIUM OR E. COLI
    5.
    发明申请
    SHUTTLE VECTOR FOR CORYNEBACTERIUM OR E. COLI 有权
    玉米。。。。。。。。

    公开(公告)号：US20160115491A1

    公开(公告)日：2016-04-28

    申请号：US14637355

    申请日：2015-03-03

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Han Min WOO ,  Youngsoon UM ,  Yunje KIM ,  Gyeong Taek GONG ,  Jung Seok LEE

    IPC: C12N15/77 ,  C12N15/72

    CPC classification number: C12N15/77 ,  C12N15/72

    Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

    Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。

    STRAIN FOR PRODUCING SUCCINATE FROM CARBON DIOXIDE AND METHOD FOR SUCCINATE PRODUCTION USING THE STRAIN
    6.
    发明申请
    STRAIN FOR PRODUCING SUCCINATE FROM CARBON DIOXIDE AND METHOD FOR SUCCINATE PRODUCTION USING THE STRAIN 审中-公开
    用于从二氧化碳生产蔗糖的菌株和使用菌株进行生产的方法

    公开(公告)号：US20160002677A1

    公开(公告)日：2016-01-07

    申请号：US14494930

    申请日：2014-09-24

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Han Min WOO ,  Jung Seok LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Byoung Koun MIN ,  Jeong-Myeong HA ,  Yunje KIM ,  Sang Jun SIM

    IPC: C12P7/46 ,  C12N15/77

    CPC classification number: C12P7/46 ,  C12N15/77

    Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

    Abstract translation: 本公开涉及使用在二氧化碳作为直接碳源而不将其转化为葡萄糖而生长的微藻中积累的淀粉生产琥珀酸的菌株，以及使用该淀粉生产琥珀酸盐的方法。 本公开提供了从二氧化碳生产琥珀酸盐的菌株，其选自谷氨酸棒杆菌BL-1-pBlAmyS（KCTC 12585BP）和谷氨酸棒杆菌BL-1-pSbAmyA（KCTC 12587BP）。 本公开还提供了一种从二氧化碳生产琥珀酸的方法，包括通过在含淀粉培养基中从二氧化碳接种产生琥珀酸的菌株来发酵淀粉。

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