公开(公告)号：US12138283B2

公开(公告)日：2024-11-12

申请号：US16413278

申请日：2019-05-15

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang-Heon Kim ,  Seokheon Hong ,  Seung Ja Oh ,  Kwi Deok Park

IPC: A61K35/28 ,  A61K9/16 ,  C12N5/0775

Abstract: Provided is a method of preparing a three-dimensional cell spheroid, the method including forming the cell spheroid by co-culturing adipose-derived stem cells or mesenchymal stem cells with hepatocytes. According to the cell spheroid prepared by the method, the secretome secreted by the adipose-derived stem cells affects hepatocyte maturation, and therefore, hepatic functions of the finally formed three-dimensional cell spheroid, i.e., organoid, may be enhanced. Further, a composition including a culture medium of the adipose-derived stem cells may prevent or treat liver diseases including hepatitis, hepatotoxicity, cholestasis, fatty liver, etc., and may enhance hepatic functions.