公开(公告)号：US12138283B2

公开(公告)日：2024-11-12

申请号：US16413278

申请日：2019-05-15

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang-Heon Kim ,  Seokheon Hong ,  Seung Ja Oh ,  Kwi Deok Park

IPC: A61K35/28 ,  A61K9/16 ,  C12N5/0775

Abstract: Provided is a method of preparing a three-dimensional cell spheroid, the method including forming the cell spheroid by co-culturing adipose-derived stem cells or mesenchymal stem cells with hepatocytes. According to the cell spheroid prepared by the method, the secretome secreted by the adipose-derived stem cells affects hepatocyte maturation, and therefore, hepatic functions of the finally formed three-dimensional cell spheroid, i.e., organoid, may be enhanced. Further, a composition including a culture medium of the adipose-derived stem cells may prevent or treat liver diseases including hepatitis, hepatotoxicity, cholestasis, fatty liver, etc., and may enhance hepatic functions.

公开(公告)号：US11667891B2

公开(公告)日：2023-06-06

申请号：US16898072

申请日：2020-06-10

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hojeong Jeon ,  Youngmin Seo ,  Hyunseon Seo ,  Myoung-Ryul Ok ,  Yu Chan Kim ,  Hyun Kwang Seok ,  Seung Ja Oh

IPC: C12N5/0786 ,  C12M1/42 ,  C12M1/34 ,  C12M1/12 ,  C12N5/078

CPC classification number: C12N5/0645 ,  C12M25/02 ,  C12M35/04 ,  C12M41/40 ,  C12N5/0643 ,  C12N2506/115 ,  C12N2521/00 ,  C12N2533/30

Abstract: Provided is a method for the selective differentiation into M1 macrophages under a pressurized environment, and more particularly, a method for the selective differentiation of undifferentiated macrophages into M1 macrophages, the method including incubating the undifferentiated macrophages in an incubator under the pressurized environment. In addition, provided is a method for producing osteoclasts, the method including: incubating undifferentiated macrophages in an incubator under a pressurized environment to differentiate into M1 macrophages; and differentiating the differentiated M1 macrophages into osteoclasts.

公开(公告)号：US11241517B2

公开(公告)日：2022-02-08

申请号：US16165515

申请日：2018-10-19

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Heon Kim ,  Joo Young Lee ,  Seung Ja Oh

IPC: A61L27/24 ,  B33Y70/00 ,  B29C64/40 ,  A61L27/20 ,  A61L27/22 ,  A61L27/26 ,  A61L27/38 ,  A61L27/52 ,  B33Y10/00 ,  B29K105/00

Abstract: Provided are a hydrogel composition with high viscoelasticity and a bioink composition including the hydrogel composition. The hydrogel composition according to an embodiment is composed of natural biocompatible substances and thus is not toxic, but has high viscosity, resulting in high mechanical stability or long persistence. Thus, the hydrogel composition may be usefully utilized as a bioink composition for bioprinting, a support in tissue engineering, or a soft tissue filler.

公开(公告)号：US12005152B2

公开(公告)日：2024-06-11

申请号：US17168780

申请日：2021-02-05

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang-Heon Kim ,  Seung Ja Oh ,  Ha Eun Chung

IPC: A61L26/00

CPC classification number: A61L26/0033 ,  A61L26/0023 ,  A61L26/008 ,  A61L2300/236 ,  A61L2300/252 ,  A61L2300/412 ,  A61L2300/60 ,  A61L27/26 ,  C08L89/06 ,  A61L27/26 ,  C08L5/08

Abstract: The present disclosure relates to a microgel composition comprising collagen and hyaluronic acid and a method of preparing the same. The microgel according to an embodiment is composed of natural biocompatible substances and is non-toxic and has high mechanical stability or long persistence, and thus can be effectively used in a microgel composition, a three-dimensional scaffold composition, and a tissue-engineering scaffold.