公开(公告)号：US20170342439A1

公开(公告)日：2017-11-30

申请号：US15326083

申请日：2015-07-16

申请人： KYOTO UNIVERSITY

发明人： Hirohide Saito ,  Kei Endo ,  Shota Katayama ,  Callum Parr

IPC分类号： C12N15/85 ,  C12N5/074

CPC分类号： C12N15/85 ,  C12N5/0619 ,  C12N5/0696 ,  C12N2501/385 ,  C12N2501/65 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/50

摘要： A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.

公开(公告)号：US10604770B2

公开(公告)日：2020-03-31

申请号：US15326083

申请日：2015-07-16

申请人： KYOTO UNIVERSITY

发明人： Hirohide Saito ,  Kei Endo ,  Shota Katayama ,  Callum Parr

IPC分类号： C12N15/85 ,  C12N5/0793 ,  C12N5/074

摘要： A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.