公开(公告)号：US20240150691A1

公开(公告)日：2024-05-09

申请号：US18280659

申请日：2022-03-09

Applicant: Kyoto University ,  OSAKA UNIVERSITY

Inventor： Kazuo TAKAYAMA ,  Yusuke TORISAWA ,  Kenji OSAFUNE ,  Maki KOTAKA ,  Sayaka DEGUCHI ,  Hiroyuki MIZUGUCHI

IPC: C12M3/00 ,  C12M1/00 ,  C12M3/06

CPC classification number: C12M21/08 ,  C12M23/16 ,  C12M29/04

Abstract: There is provided a bile duct chip that is a bile duct chip (200) including a membrane (210) through which substances are movable from one surface to the other surface, a first substrate (221) having a recessed part that forms a first flow path (220), and a second substrate (231) having a recessed part that forms a second flow path (230), in which the first substrate (221), the membrane (210), the second substrate (231) are stacked in this order, the recessed part of the first substrate (221) has an opening portion facing the one surface of the membrane (210), the recessed part of the second substrate has an opening portion facing the other surface of the membrane (210), the one surface of the membrane (210) forms a part of the first flow path (220), the other surface of the membrane (210) forms a part of the second flow path (230), the first flow path (220) and the second flow path (230) communicate with each other through the membrane (210), and bile duct epithelial cells (222) are disposed on a surface of an inner wall of the first flow path (220) to form a tube.

公开(公告)号：US20180119095A1

公开(公告)日：2018-05-03

申请号：US15539042

申请日：2015-12-25

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Maki KOTAKA

IPC: C12N5/071 ,  G01N33/50

CPC classification number: C12N5/067 ,  C12N2500/30 ,  C12N2500/62 ,  C12N2501/12 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/31 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/80 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5067

Abstract: A method for producing hepatocytes from hepatoblasts is provided. The method includes the step of culturing the hepatoblasts in a medium containing a compound selected from the group consisting of pregnenolone and an adrenergic agonist. The hepatoblasts can be obtained by culturing endodermal cells in a medium containing DMSO, and the endodermal cells can be obtained by culturing pluripotent stem cells in a medium containing Activin A and a GSK-3β inhibitor. Accordingly, a method for producing hepatocytes from pluripotent stem cells is also provided by employing the method of the present invention.