公开(公告)号：US20240043811A1

公开(公告)日：2024-02-08

申请号：US18487457

申请日：2023-10-16

Applicant: KYOTO UNIVERSITY

Inventor： Shinichi SUETA ,  Tomoko KASAHARA ,  Kenji OSAFUNE

IPC: C12N5/071 ,  C12N5/074

CPC classification number: C12N5/0687 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/998 ,  C12N2533/52

Abstract: A method of producing a mesodermal-lineage primitive streak cell includes a first step of culturing pluripotent stem cells in a medium containing FGF2, BMP (bone morphogenetic protein) 4, a GSK-3β inhibitor, and retinoic acid or a derivative thereof and a second step of culturing cells obtained in the first step in a medium containing FGF2, a GSK-3β inhibitor, and BMP7.

公开(公告)号：US20200248148A1

公开(公告)日：2020-08-06

申请号：US16616942

申请日：2018-05-23

Applicant: KYOTO UNIVERSITY

Inventor： Shinichi SUETA ,  Tomoko KASAHARA ,  Kenji OSAFUNE

IPC: C12N5/071 ,  C12N5/074

Abstract: A method of producing renal progenitor cells from pluripotent stem cells involves culturing pluripotent stem cells in a medium containing FGF2, BMP4, a GSK-3β inhibitor, and retinoic acid or a derivative thereof, culturing the resulting cells in a medium containing FGF2, a GSK-3β inhibitor, and BMP7, culturing the resulting cells in a medium containing FGF2, a GSK-3β inhibitor, BMP7, and a TGFβ inhibitor, culturing the resulting cells in a medium containing FGF2, a GSK-3β inhibitor, BMP7, activin, and a ROCK (Rho-kinase) inhibitor, culturing the resulting cells in a medium containing retinoic acid or a derivative thereof, and FGF9, and culturing the resulting cells in a medium containing a GSK-3β inhibitor and FGF9, to induce renal progenitor cells from intermediate mesodermal cells.