公开(公告)号：US20240010989A1

公开(公告)日：2024-01-11

申请号：US18271112

申请日：2022-01-11

Applicant: Kyoto University ,  Rege Nephro Co., Ltd.

Inventor： Kenji OSAFUNE ,  Toshikazu ARAOKA ,  Yoshifumi KOBAYASHI ,  Makoto RYOSAKA ,  Hiraku TSUJIMOTO

IPC: C12N5/071

CPC classification number: C12N5/0687 ,  C12N2506/45 ,  C12N2501/119 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/15

Abstract: A medium for culturing and expanding nephron progenitor cells, the medium containing a GSK-3β inhibitor, a ROCK inhibitor, and at least one fibroblast growth factor selected from the group consisting of FGF9 and FGF20. Also, a method for culturing and expanding nephron progenitor cells using the medium. Also, a method for producing renal organoids, the method including a step of culturing and expanding nephron progenitor cells using the above method for culturing and expanding nephron progenitor cells, and a step of differentiating the cultured and expanded nephron progenitor cells into renal organoids.

公开(公告)号：US20210332329A1

公开(公告)日：2021-10-28

申请号：US17262398

申请日：2019-07-22

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Toshikazu ARAOKA ,  Akira WATANABE

IPC: C12N5/071 ,  G01N33/569

Abstract: A method for concentrating renal progenitor cells involves extracting MET-positive cells and/or AGTR2-positive cells from a renal progenitor cell-containing cell population. The MET-positive cells and/or AGTR2-positive cells are extracted using an anti-MET antibody and/or an anti-AGTR2 antibody. The renal progenitor cell-containing cell population is obtained from pluripotent stem cells. The renal progenitor cells are OSR1 (odd-skipped related 1)-positive and SIX2 (SIX Homeobox 2)-positive.