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公开(公告)号：US10329250B2
公开(公告)日：2019-06-25
申请号：US15515142
申请日：2015-10-08
摘要： According to the present invention, there can be provided a process for producing 7-dehydrocholesterol (7DHC), comprising culturing, in a medium, a 7DHC-producing Labyrinthulea microorganism in which sterol 24-C-methyltransferase activity is reduced or lost as compared to a parent strain, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, 7-dehydrocholesterol produced by the production process.
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公开(公告)号：US10252991B2
公开(公告)日：2019-04-09
申请号：US15110827
申请日：2015-01-14
IPC分类号： C12P33/00 , C07C401/00 , C07J9/00
摘要： According to the present invention, there can be provided A process for producing 7-dehydrocholesterol (hereinafter, “7DHC”), comprising culturing, in a medium, a 7DHC-producing Labyrinthulea microorganism in which the 7DHC reducing activity is reduced or lost as compared to a parent strain through deletion, substitution, or addition of at least one base in a gene which is present in the chromosomal DNA of the parent strain and encodes a protein having 7DHC reducing activity, and the microorganism produces 7DHC, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, the 7DHC produced by the production process.
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公开(公告)号：US20160332964A1
公开(公告)日：2016-11-17
申请号：US15110827
申请日：2015-01-14
IPC分类号： C07C401/00 , C12P33/00
CPC分类号： C07C401/00 , C07C2601/14 , C07C2602/24 , C07J9/00 , C12P33/00
摘要： According to the present invention, there can be provided A process for producing 7-dehydrocholesterol (hereinafter, “7DHC”), comprising culturing, in a medium, a 7DHC-producing Labyrinthulea microorganism in which the 7DHC reducing activity is reduced or lost as compared to a parent strain through deletion, substitution, or addition of at least one base in a gene which is present in the chromosomal DNA of the parent strain and encodes a protein having 7DHC reducing activity, and the microorganism produces 7DHC, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, the 7DHC produced by the production process.
摘要翻译： 根据本发明，可以提供一种制备7-脱氢胆固醇（以下称为“7DHC”）的方法，其包括在培养基中培养7DHC生产的迷迭虫微生物，其中7DHC还原活性减少或丧失，相比之下 通过在亲本菌株的染色体DNA中存在的基因中的至少一个碱基的缺失，取代或添加而编码亲本菌株，并编码具有7DHC还原活性的蛋白质，并且该微生物产生7DHC，允许产生7DHC 并积累在文化中，从文化中收集7DHC; 以及生产维生素D3的方法，包括用紫外线照射由生产过程产生的7DHC。
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4.发明授权公开(公告)号：US11613728B2
公开(公告)日：2023-03-28
申请号：US17266984
申请日：2019-08-09
发明人： Tohru Dairi , Yasuharu Satoh , Shohei Hayashi , Mai Naka , Tetsuro Ujihara
摘要： An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.
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公开(公告)号：US10023884B2
公开(公告)日：2018-07-17
申请号：US14760290
申请日：2014-01-17
发明人： Tetsuro Ujihara , Megumi Nagano , Kazuhiko Tabata
摘要： According to the present invention, a microorganism belonging to the genus Aurantiochytrium having an 18S rRNA gene consisting of the base sequence represented by any of SEQ ID NOS: 1 to 5; Aurantiochytrium sp. OH4 strain; a microorganism which is a mutant obtained from the above-mentioned microorganism as a parent strain and has a higher ability to produce DHA than the parent strain; or Aurantiochytrium sp. LTR23 strain is provided. Also, a method for producing a DHA-containing composition, DHA and DHA alkyl ester by a fermentation process using the above-mentioned microorganisms is provided.
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6.发明申请公开(公告)号：US20150353972A1
公开(公告)日：2015-12-10
申请号：US14760290
申请日：2014-01-17
发明人： Tetsuro Ujihara , Megumi Nagano , Kazuhiko Tabata
CPC分类号： C12P7/6427 , C12N1/12 , C12P7/6472 , C12R1/89
摘要： According to the present invention, a microorganism belonging to the genus Aurantiochytrium having an 18S rRNA gene consisting of the base sequence represented by any of SEQ ID NOS: 1 to 5; Aurantiochytrium sp. OH4 strain; a microorganism which is a mutant obtained from the above-mentioned microorganism as a parent strain and has a higher ability to produce DHA than the parent strain; or Aurantiochytrium sp. LTR23 strain is provided. Also, a method for producing a DHA-containing composition, DHA and DHA alkyl ester by a fermentation process using the above-mentioned microorganisms is provided.
摘要翻译： 根据本发明，属于Aurantiochytrium属的微生物，其具有由SEQ ID NO：1至5中任一个表示的碱基序列组成的18S rRNA基因; 紫rium属 OH4菌株; 作为亲本菌株的上述微生物获得的突变体，比亲本菌株具有更高的产生DHA的能力的微生物; 或Aurantiochytrium sp。 提供LTR23菌株。 此外，提供了通过使用上述微生物的发酵方法来生产含DHA的组合物DHA和DHA烷基酯的方法。
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