公开(公告)号：US10376599B2

公开(公告)日：2019-08-13

申请号：US15559543

申请日：2016-03-18

Applicant: Kansas State University Research Foundation

Inventor： Stefan H. Bossmann ,  Deryl L. Troyer ,  Aruni P. Malalasekera ,  Hongwang Wang ,  Sebastian O. Wendel ,  Gaohong Zhu

IPC: A61K38/00 ,  A61K49/00 ,  G01N33/573 ,  C12Q1/26 ,  C12Q1/34

Abstract: A nanoplatform assembly for detection of arginase, indoleamine 2,3-dioxygenase 1, and/or tryptophan 2,3-dioxygenase. The nanoplatform comprises an oligopeptide, which is used as a linker between two particles. More preferably, the linker is comprised of an oligopeptide containing a substrate for the target enzyme, where the substrate is chemically or physically modified by the target enzyme (but not cleaved). A central particle with a plurality of oligopeptide-tethered detectable particles and a plurality of directly attached detectable particles is described. Posttranslational modification of the oligopeptide leads to changes in the detectable signals from the first and/or second particles in the nanoplatform, which can be correlated to enzyme activity and concentration.

公开(公告)号：US20180099057A1

公开(公告)日：2018-04-12

申请号：US15559543

申请日：2016-03-18

Applicant: Kansas State University Research Foundation

Inventor： Stefan H. Bossmann ,  Deryl L. Troyer ,  Aruni P. Malalasekera ,  Hongwang Wang ,  Sebastian O. Wendel ,  Gaohong Zhu

IPC: A61K49/00 ,  C12Q1/34 ,  C12Q1/26

CPC classification number: A61K49/0093 ,  A61K49/0032 ,  A61K49/0036 ,  A61K49/0056 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y113/11011 ,  C12Y113/11052 ,  C12Y305/03001 ,  G01N33/573

Abstract: A nanoplatform assembly for detection of arginase, indoleamine 2,3-dioxygenase 1, and/or tryptophan 2,3-dioxygenase. The nanoplatform comprises an oligopeptide, which is used as a linker between two particles. More preferably, the linker is comprised of an oligopeptide containing a substrate for the target enzyme, where the substrate is chemically or physically modified by the target enzyme (but not cleaved). A central particle with a plurality of oligopeptide-tethered detectable particles and a plurality of directly attached detectable particles is described. Posttranslational modification of the oligopeptide leads to changes in the detectable signals from the first and/or second particles in the nanoplatform, which can be correlated to enzyme activity and concentration.