公开(公告)号：US07112571B2

公开(公告)日：2006-09-26

申请号：US10266609

申请日：2002-10-09

申请人： Katsuhiro Osajima ,  Eiji Seki ,  Mayumi Yoshida

发明人： Katsuhiro Osajima ,  Eiji Seki ,  Mayumi Yoshida

IPC分类号： A61K38/05

CPC分类号： A23J1/04 ,  A23L2/66 ,  A23L33/18 ,  A61K38/00 ,  C07K14/461

摘要： Peptide which is obtained by treating fish meat such as sardine with an alkaline protease is adsorbed with a peptide-adsorbing resin (such as ODS resin) and eluted with 10 aqueous ethanol to obtain a peptide (Y-2 fraction). The resulting novel peptide has an excellent hypotensive action and can be used as a nutrient and a specific health food and, in addition, it can be advantageously used as a hypotensive agent.

摘要翻译： 通过用碱性蛋白酶处理鱼肉如沙丁鱼获得的肽被肽吸附树脂（例如ODS树脂）吸附并用10％乙醇水溶液洗脱，得到肽（Y-2级分）。 所得的新型肽具有优异的降血压作用，可用作营养物质和特定保健食品，另外还可有利地用作降血压剂。

公开(公告)号：US4853231A

公开(公告)日：1989-08-01

申请号：US43922

申请日：1987-04-29

申请人： Kazuharu Osajima ,  Katsuhiro Osajima

发明人： Kazuharu Osajima ,  Katsuhiro Osajima

IPC分类号： A23L27/21 ,  A23J1/02 ,  A23J1/04 ,  A23J3/34

CPC分类号： A23J1/04 ,  A23J1/02 ,  A23J3/341

摘要： Tastable matters consisting mainly of low molecular weight peptides are obtained from raw material meats of fishes, shellfishes, birds, beasts or whales, by a method including a step of finely pulverizing the raw material meat followed by decomposing the pulverized meat with the autolyzing enzymes of the said meat material, a step of adding other protein-decomposing enzymes when the autolyzing reaction speed has reached the maximum value so that the protein-decomposition reactions by the added enzymes and the previous autolyzing enzymes may proceed simultaneously, and a step of deactivating the both enzymes followed by purification and concentration of the resulting low molecular peptide-containing tastable matter. In order to impove the quality of the tastable matter thus obtained, the reaction solution as obtained in the previous autolysis step and an amino acid are added to the concentrated low molecular peptide-containing solution and again reacted therebetween and then the enzymes used are deacrivated, or alternatively, an amino acid only is added to the freshly purified low molecular peptide-containing solution and reacted whereupon the solution is continuously stirred without the liquid temperature thereof being lowered. The tastable matters thus obtained have the native aroma and umami of the raw material meats.

摘要翻译： 主要由低分子量肽组成的可口味由鱼类，贝类，鸟类，野兽或鲸鱼的原料肉类获得，包括以下步骤：将原料肉细粉碎，然后用自动分解酶分解粉碎肉 所述肉类材料，当自动结晶反应速度达到最大值时添加其它蛋白质分解酶的步骤，使得通过添加的酶和先前的自动分解酶的蛋白质分解反应可以同时进行，并且使 然后将两种酶纯化并浓缩得到的含低分子肽的味觉物质。 为了消除由此获得的可口味物质的质量，将上述自溶步骤中获得的反应溶液和氨基酸加入到含浓缩的低分子量肽溶液中，并再次反应，然后将所用酶脱泡， 或者，仅将新氨基酸加入到新鲜纯化的含低分子肽溶液中并反应，然后在不降低其液体温度的情况下连续搅拌该溶液。 因此获得的味道具有原料肉的天然香气和鲜味。

公开(公告)号：US06905704B2

公开(公告)日：2005-06-14

申请号：US10472062

申请日：2002-12-24

申请人： Katsuhiro Osajima ,  Yasunori Ooishi

发明人： Katsuhiro Osajima ,  Yasunori Ooishi

IPC分类号： A23J1/04 ,  A23L1/305 ,  A23L2/66 ,  A61K38/00 ,  A61K38/01 ,  A61K38/05 ,  A61K38/55 ,  A61P9/12 ,  A61P43/00 ,  C07K1/20 ,  C07K2/00 ,  C07K5/06 ,  C07K14/46 ,  C07K5/062

CPC分类号： C07K5/06052 ,  A23J1/04 ,  A23L2/66 ,  A23L33/18 ,  A61K38/012 ,  A61K38/05 ,  A61K38/556 ,  C07K2/00 ,  C07K14/461 ,  Y10S530/857

摘要： Peptide SY is produced by applying a mixture of peptides obtained by processing fish meat with a protease to a peptide-adsorbing resin (ODS resin or the like), eluting this with water, then with a 11 to 18% v/v ethanol aqueous solution and further with water, and collecting and mixing a latter fraction of the water elution (1), a fraction of the 11 to 18 % v/v ethanol elution and a fraction of the water elution (2). Further, peptide SY-MD is produced by isolating only the fraction of the 11 to 18% v/v ethanol elution. Peptide SY and peptide SY-MD are both novel peptide mixtures. They not only contain a large amount of blood pressure-depressing peptide Val-Tyr newly found but also have less bitterness and are excellent in taste and stability, and can be used as a blood pressure-depressing agent or as functional foods for inhibiting blood pressure elevation or preventing blood pressure elevation.

摘要翻译： 肽SY通过将通过将鱼肉加蛋白酶获得的肽的混合物施加到肽吸附树脂（ODS树脂等）上，用水洗脱，然后用11至18％v / v乙醇水溶液 并进一步用水，并收集和混合水洗脱（1）的后一部分，11至18％v / v乙醇洗脱的一部分和水洗脱的部分（2）。 此外，通过仅分离11至18％v / v乙醇洗脱的级分来产生肽SY-MD。 肽SY和肽SY-MD都是新的肽混合物。 它们不仅含有大量新近发现的降压抑制肽Val-Tyr，而且具有较少的苦味，味道和稳定性优异，可用作降压药或用作抑制血压的功能性食品 升高或防止血压升高。

公开(公告)号：US20060040872A1

公开(公告)日：2006-02-23

申请号：US11058162

申请日：2005-02-16

申请人： Katsuhiro Osajima ,  Kiyoshi Matsumoto ,  Toshiro Matsui

发明人： Katsuhiro Osajima ,  Kiyoshi Matsumoto ,  Toshiro Matsui

IPC分类号： A61K38/04 ,  C12P21/06

CPC分类号： A61K38/05 ,  A23L2/52 ,  A23L2/66 ,  A23L33/18

摘要： The present invention relates to an agent which inhibits or suppresses calcium channel where dipeptide Val-Tyr (VY) or a substance containing the same is an effective ingredient. Examples of the substance containing VY are hydrolysate (sardine peptide) of fish meat of sardine, etc., and VY or VY-containing substance is also able to be used as food/beverage in addition to pharmaceuticals. In accordance with the present invention, it has been clarified that, in reduction of blood pressure, VY has two actions of calcium channel inhibition in addition to ACE inhibition.

摘要翻译： 本发明涉及抑制或抑制钙通道的药剂，其中二肽Val-Tyr（VY）或其含有物质是有效成分。 含有VY的物质的实例是沙丁鱼等的鱼肉的水解物（沙丁鱼肽）等，除了药物之外，含有VY或VY的物质也可以用作食品/饮料。 根据本发明，已经说明，在降低血压方面，除了ACE抑制之外，VY还具有钙通道抑制的两种作用。