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1.发明授权Recombinant microorganism that expresses a secY gene with deletion of sporulation-associated genes and method of producing thereof 有权表达具有孢子形成相关基因缺失的secY基因的重组微生物及其制备方法公开(公告)号：US08389264B2
公开(公告)日：2013-03-05
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
IPC分类号： C12N1/21
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided.A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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公开(公告)号：US20090029417A1
公开(公告)日：2009-01-29
申请号：US12279271
申请日：2007-02-15
申请人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
发明人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
CPC分类号： C12N9/2437
摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.
摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。
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公开(公告)号：US20110151567A1
公开(公告)日：2011-06-23
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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4.发明授权公开(公告)号：US08460893B2
公开(公告)日：2013-06-11
申请号：US12279271
申请日：2007-02-15
申请人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
发明人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
CPC分类号： C12N9/2437
摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.
摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。
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5.发明授权公开(公告)号：US09705396B2
公开(公告)日：2017-07-11
申请号：US14784028
申请日：2013-12-18
申请人： Shenghao Liu
发明人： Shenghao Liu
CPC分类号： H02M1/42 , H02M1/4225 , H02M1/44 , H02M3/157 , H02M7/53873 , H02M2001/0012 , H02M2001/327 , Y02B70/126
摘要： An electronic converter circuit system and a control method are disclosed. The electronic converter circuit system has a digital control platform, centered on a digital control chip, consisting of a hardware circuit and software, and built in with single-end, push-pull, half-bridge, full-bridge type control modes. By simple setting, the circuit system works in corresponding modes. The electronic converter circuit system mainly controls the field-effect tubes and IGBT. Control orders in the control process are output after comprehensive diagnosis of the modules by the software control platform. During conversion, the circuit works stably and has high conversion efficiency. Besides, in the control process of the electronic converter circuit system, the environmental parameters such as the temperature, humidity, altitude and intensity of the electromagnetic field of the working environment of the circuit system are tested, and various environmental factors are balanced to ensure that the whole circuit system works safely.
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