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    METHODS FOR PRODUCING SECRETED PROTEINS
    1.
    发明申请
    METHODS FOR PRODUCING SECRETED PROTEINS 有权
    生产分泌蛋白的方法

    公开(公告)号：US20100319090A1

    公开(公告)日：2010-12-16

    申请号：US12503615

    申请日：2009-07-15

    申请人： Keiko Migiwa ,  Yutaka Matsushima ,  Akitsu Nagasawa

    发明人： Keiko Migiwa ,  Yutaka Matsushima ,  Akitsu Nagasawa

    IPC分类号： C12P21/00 ,  C12N15/63 ,  C07H21/04 ,  C12N5/10 ,  A01H5/00

    CPC分类号： C12N15/8221 ,  C12N15/8201

    摘要： The present invention relates to a method for producing a heterologous protein secreted out of a plant cell comprising introducing into a plant cell genome a DNA encoding an amino acid sequence that comprises a glycinin signal sequence for endoplasmic reticulum transport and an amino acid sequence of a heterologous protein, wherein the signal sequence is directly fused to the amino acid sequence of the heterologous protein or one or two amino acids are inserted between the signal sequence and the amino acid sequence of the heterologous protein; and expressing the DNA.

    摘要翻译： 本发明涉及从植物细胞分泌的异源蛋白质的制造方法，包括向植物细胞基因组中导入编码包含用于质粒内转运的大豆球蛋白信号序列和异源氨基酸序列的氨基酸序列的DNA 蛋白质，其中信号序列直接融合到异源蛋白质的氨基酸序列，或者一个或两个氨基酸插入在信号序列和异源蛋白质的氨基酸序列之间; 并表达DNA。

    Glycinin signal sequence for producing secreted proteins in plants
    2.
    发明授权
    Glycinin signal sequence for producing secreted proteins in plants 有权
    用于在植物中产生分泌蛋白的大豆球蛋白信号序列

    公开(公告)号：US08637316B2

    公开(公告)日：2014-01-28

    申请号：US12503615

    申请日：2009-07-15

    申请人： Keiko Migiwa ,  Yutaka Matsushima ,  Akitsu Nagasawa

    发明人： Keiko Migiwa ,  Yutaka Matsushima ,  Akitsu Nagasawa

    IPC分类号： C12N15/82 ,  A01H5/00 ,  C07H21/04

    CPC分类号： C12N15/8221 ,  C12N15/8201

    摘要： The present invention relates to a method for producing a heterologous protein secreted out of a plant cell comprising introducing into a plant cell genome a DNA encoding an amino acid sequence that comprises a glycinin signal sequence for endoplasmic reticulum transport and an amino acid sequence of a heterologous protein, wherein the signal sequence is directly fused to the amino acid sequence of the heterologous protein or one or two amino acids are inserted between the signal sequence and the amino acid sequence of the heterologous protein; and expressing the DNA.

    摘要翻译： 本发明涉及从植物细胞分泌的异源蛋白质的制造方法，包括向植物细胞基因组中导入编码包含用于质粒内转运的大豆球蛋白信号序列和异源氨基酸序列的氨基酸序列的DNA 蛋白质，其中信号序列直接融合到异源蛋白质的氨基酸序列，或者一个或两个氨基酸插入在信号序列和异源蛋白质的氨基酸序列之间; 并表达DNA。

    Herbicidal compound resistant plant
    3.
    发明授权
    Herbicidal compound resistant plant 有权
    除草复合抗性植物

    公开(公告)号：US07563950B2

    公开(公告)日：2009-07-21

    申请号：US11130391

    申请日：2005-05-17

    申请人： Yutaka Matsushima ,  Akitsu Nagasawa

    发明人： Yutaka Matsushima ,  Akitsu Nagasawa

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8274

    摘要： The present invention provides a plant on which resistance to at least one of protoporphyrinogen IX oxidize inhibitory-type herbicidal compounds is conferred, wherein to said plant (1) a DNA encoding a protein showing protoporphyrinogen IX oxidize activity and (2) a DNA encoding cytochrome P450 showing activity of metabolizing said herbicidal compound, have been introduced and expressed, and the like.

    摘要翻译： 本发明提供了赋予至少一种原卟啉原IX氧化抑制型除草化合物的植物，其中所述植物（1）编码显示原卟啉原IX氧化活性的蛋白质的DNA和（2）编码细胞色素的DNA 显示代表所述除草化合物的活性的P450已被引入和表达等。

    Method for controlling weeds
    4.
    发明申请
    Method for controlling weeds 有权

    公开(公告)号：US20060009361A1

    公开(公告)日：2006-01-12

    申请号：US11129574

    申请日：2005-05-16

    申请人： Yutaka Matsushima ,  Akitsu Nagasawa

    发明人： Yutaka Matsushima ,  Akitsu Nagasawa

    IPC分类号： A01N43/54

    CPC分类号： A01N43/58 ,  A01N43/54 ,  A01N43/60 ,  A01N43/653

    摘要： The present invention provides a method for controlling weeds comprising a step of applying one or more compounds to a cultivation area of a plant, to which a gene of cytochrome P450 showing activity of metabolizing a protoporphyrinogen IX oxidase inhibitory-type herbicidal compound has been introduced, wherein said compound to be applied to said area is selected from the group consisting of: (1) flufenpyr-ethyl, (2) 2-[4-chloro-2-fluoro-5-(2-propynyloxy)phenyl]-4-methy-5-(trifluoromethyl)-3(2H)-pyridazinone, (3) carfentrazone-ethyl, and (4) sulfentrazone; and the like.

    Herbicidal compound resistant plant
    5.
    发明申请
    Herbicidal compound resistant plant 有权
    除草复合抗性植物

    公开(公告)号：US20060005268A1

    公开(公告)日：2006-01-05

    申请号：US11130391

    申请日：2005-05-17

    申请人： Yutaka Matsushima ,  Akitsu Nagasawa

    发明人： Yutaka Matsushima ,  Akitsu Nagasawa

    IPC分类号： A01H1/00 ,  C12N15/82 ,  A01N63/00

    CPC分类号： C12N15/8274

    摘要： The present invention provides a plant on which resistance to at least one of protoporphyrinogen IX oxidase inhibitory-type herbicidal compounds is conferred, wherein to said plant (1) a DNA encoding a protein showing protoporphyrinogen IX oxidase activity and (2) a DNA encoding cytochrome P450 showing activity of metabolizing said herbicidal compound, have been introduced and expressed, and the like.

    摘要翻译： 本发明提供了赋予至少一种原卟啉原IX氧化酶抑制型除草化合物的抗性的植物，其中所述植物（1）编码显示原卟啉原IX氧化酶活性的蛋白质的DNA和（2）编码细胞色素的DNA 显示代表所述除草化合物的活性的P450已被引入和表达等。

    Method for controlling weeds
    6.
    发明授权
    Method for controlling weeds 有权
    杂草防治方法

    公开(公告)号：US07521596B2

    公开(公告)日：2009-04-21

    申请号：US11129574

    申请日：2005-05-16

    申请人： Yutaka Matsushima ,  Akitsu Nagasawa

    发明人： Yutaka Matsushima ,  Akitsu Nagasawa

    IPC分类号： C12N15/82

    CPC分类号： A01N43/58 ,  A01N43/54 ,  A01N43/60 ,  A01N43/653

    摘要： The present invention provides a method for controlling weeds comprising a step of applying one or more compounds to a cultivation area of a plant, to which a gene of cytochrome P450 showing activity of metabolizing a protoporphyrinogen IX oxidase inhibitory-type herbicidal compound has been introduced, wherein said compound to be applied to said area is selected from the group consisting of: (1) flufenpyr-ethyl, (2) 2-[4-chloro-2-fluoro-5-(2-propynyloxy)phenyl]-4-methy-5-(trifluoromethyl)-3(2H)-pyridazinone, (3) carfentrazone-ethyl, and (4) sulfentrazone; and the like.

    摘要翻译： 本发明提供了一种控制杂草的方法，包括将一种或多种化合物施用于植物的培养区域的步骤，其中引入了代表原卟啉原IX氧化酶抑制型除草化合物的活性的细胞色素P450基因， 其中所述施用于所述区域的化合物选自：（1）氟苯吡啶 - 乙基，（2）2- [4-氯-2-氟-5-（2-丙炔氧基）苯基] -4- 甲基-5-（三氟甲基）-3（2H） - 哒嗪酮，（3）氟酮唑酮 - 乙基和（4）磺胺腙; 等等。

    METHOD FOR CULTIVATING CROP PLANT
    7.
    发明申请
    METHOD FOR CULTIVATING CROP PLANT 审中-公开
    栽培作物栽培方法

    公开(公告)号：US20120149576A1

    公开(公告)日：2012-06-14

    申请号：US13377115

    申请日：2010-06-09

    申请人： Takanori Saijo ,  Akitsu Nagasawa

    发明人： Takanori Saijo ,  Akitsu Nagasawa

    IPC分类号： A01N43/54 ,  A01P13/00 ,  C12Q1/26

    CPC分类号： C12N15/8274 ,  A01H5/10 ,  A01N43/54 ,  A01N63/02 ,  C12N9/001 ,  C12N9/0077 ,  C12Y103/03004

    摘要： The present invention enables providing a method for cultivating a crop plant into which has been introduced either one or both of (1) DNA comprising a nucleotide sequence encoding the amino acid sequence of a cytochrome P450 that shows saflufenacil metabolizing activity and (2) DNA comprising a nucleotide sequence encoding the amino acid sequence of a protein that shows protoporphyrinogen IX oxidase activity, wherein said method comprises applying a weed control agent that contains saflufenacil as an active ingredient to an area where said crop plant is cultivated; among others.

    摘要翻译： 本发明提供了一种栽培作物植物的方法，其中已经引入了（1）包含编码显示香草氟苯代谢活性的细胞色素P450的氨基酸序列的核苷酸序列的DNA中的一种或两种，（2）包含 编码显示原卟啉原IX氧化酶活性的蛋白质的氨基酸序列的核苷酸序列，其中所述方法包括将包含香草氟苯作为活性成分的杂草控制剂施用于所述作物栽培的区域; 等等

    Method for producing transgenic plants resistant to weed control compounds which disrupt the porphyrin pathways of plants
    9.
    发明授权
    Method for producing transgenic plants resistant to weed control compounds which disrupt the porphyrin pathways of plants 失效
    用于产生抗杂草控制化合物的转基因植物的方法，其破坏植物的卟啉途径

    公开(公告)号：US07973219B2

    公开(公告)日：2011-07-05

    申请号：US12320107

    申请日：2009-01-16

    申请人： Hiroki Nakajima ,  Akitsu Nagasawa

    发明人： Hiroki Nakajima ,  Akitsu Nagasawa

    IPC分类号： C12N15/82

    CPC分类号： C07K7/06 ,  C07K5/1016 ,  C07K14/001 ,  C12N9/00 ,  C12N9/001 ,  C12N9/88 ,  C12N15/8274

    摘要： Methods for producing a transgenic plant that is resistant to a weed control compound including introducing a nucleotide sequence encoding a coproporphyrinogen III oxidase of Escherichia coli into a plant cell, expressing the nucleotide sequence within the plant cell, and regenerating the plant cell into a transgenic plant and transgenic plants produced by such methods. Methods for controlling weeds including applying a weed control compound to an area containing transgenic plants containing a nucleotide sequence encoding a coproporphyrinogen III oxidase of Escherichia coli. Methods for selecting plants or plant cells resistant to a weed control compound including applying a weed control compound to transgenic plants or transgenic plant cells containing a nucleotide sequence encoding a coproporphyrinogen III oxidase of Escherichia coli.

    摘要翻译： 用于生产对杂草对照化合物具有抗性的转基因植物的方法，包括将编码大肠杆菌的cop卟啉原III氧化酶的核苷酸序列引入植物细胞中，在植物细胞内表达核苷酸序列，并将植物细胞再生成转基因植物 和通过这些方法产生的转基因植物。 控制杂草的方法，包括将杂草控制化合物施用于含有编码大肠杆菌共卟啉原III氧化酶的核苷酸序列的转基因植物的区域。 用于选择对杂草对照化合物具有抗性的植物或植物细胞的方法，包括将杂草控制化合物施用于含有编码大肠杆菌共卟啉原III氧化酶的核苷酸序列的转基因植物或转基因植物细胞。