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公开(公告)号：US20090137406A1
公开(公告)日：2009-05-28
申请号：US12227904
申请日：2007-06-01
申请人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
发明人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
CPC分类号： C12Q1/6837
摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.
摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。
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公开(公告)号：US08088580B2
公开(公告)日：2012-01-03
申请号：US12227904
申请日：2007-06-01
申请人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
发明人： Kenji Kinoshita , Kanehisa Yokoyama , Kentaro Fujimoto , Toru Yakabe , Shin Saito , Kazuhiko Fujiwara
CPC分类号： C12Q1/6837
摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.
摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。
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3.发明申请Method of synthesizing cdna and method of synthesizing rna chains, and nucleotide-immobilized carrier 有权合成cdna的方法和合成rna链的方法以及核苷酸固定载体公开(公告)号：US20100016566A1
公开(公告)日：2010-01-21
申请号：US11990641
申请日：2006-08-09
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/686 , C12Q2521/107 , C12Q2565/537
摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。
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公开(公告)号：US07968290B2
公开(公告)日：2011-06-28
申请号：US11920559
申请日：2006-05-16
CPC分类号： C12N15/1096 , C12Q1/6834 , C12Q2565/537 , C12Q2527/107
摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.
摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。
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5.发明授权Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier 有权合成cDNA的方法和合成RNA链的方法以及核苷酸固定载体公开(公告)号：US08822670B2
公开(公告)日：2014-09-02
申请号：US11990641
申请日：2006-08-09
IPC分类号： C07H21/00
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/686 , C12Q2521/107 , C12Q2565/537
摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.
摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。
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公开(公告)号：US20090042190A1
公开(公告)日：2009-02-12
申请号：US11920559
申请日：2006-05-16
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6834 , C12Q2565/537 , C12Q2527/107
摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.
摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。
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公开(公告)号：US08435742B2
公开(公告)日：2013-05-07
申请号：US12739978
申请日：2008-11-04
CPC分类号： C12Q1/6809 , C12Q2565/537 , C12Q2525/143 , C12Q2521/107
摘要： [Object] It is to provide a method and a kit capable of detecting or quantifying a target RNA simply and rapidly from trace amounts of RNA in a sample, in a case such as when one or more kinds of pathogenic microorganisms are to be detected or quantified.[Solving Means] The method comprises the steps of 1) synthesizing cDNA from a sample containing the target RNA using a liquid-phase primer having a promoter sequence and a reverse transcriptase to obtain a cDNA-RNA complex, 2) degrading the RNA of the complex, 3) synthesizing a double-stranded DNA via the cDNA obtained in the step 2) and the solid-phase primer, 4) synthesizing RNA from the double-stranded DNA, 5) synthesizing cDNA via the RNA obtained in the step 4) and the solid-phase primer to obtain a cDNA-RNA complex, 6) degrading the RNA of the complex obtained in step 5), 7) synthesizing a double-stranded DNA via the cDNA obtained in the step 6) and the liquid-phase primer, and 8) quantifying the double-stranded DNAs obtained in the steps 3) and 7). These steps can be performed in a single reaction solution.
摘要翻译： 本发明提供一种方法和试剂盒，其能够在样品中的痕量RNA中简单快速地检测或定量靶RNA，例如当检测到一种或多种病原微生物或 量化 [解决方法]该方法包括以下步骤：1）使用具有启动子序列和逆转录酶的液相引物合成来自含有靶RNA的样品的cDNA，得到cDNA-RNA复合物，2）降解 复合物，3）通过步骤2）和固相引物获得的cDNA合成双链DNA，4）从双链DNA合成RNA，5）通过步骤4）得到的RNA合成cDNA， 和固相引物以获得cDNA-RNA复合物，6）降解步骤5）中获得的复合物的RNA，7）通过步骤6）中得到的cDNA合成双链DNA和液相 引物，和8）定量在步骤3）和7）中获得的双链DNA。 这些步骤可以在单个反应溶液中进行。
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公开(公告)号：US20110053150A1
公开(公告)日：2011-03-03
申请号：US12739978
申请日：2008-11-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2565/537 , C12Q2525/143 , C12Q2521/107
摘要： [Object] It is to provide a method and a kit capable of detecting or quantifying a target RNA simply and rapidly from trace amounts of RNA in a sample, in a case such as when one or more kinds of pathogenic microorganisms are to be detected or quantified.[Solving Means] The method comprises the steps of 1) synthesizing cDNA from a sample containing the target RNA using a liquid-phase primer having a promoter sequence and a reverse transcriptase to obtain a cDNA-RNA complex, 2) degrading the RNA of the complex, 3) synthesizing a double-stranded DNA via the cDNA obtained in the step 2) and the solid-phase primer, 4) synthesizing RNA from the double-stranded DNA, 5) synthesizing cDNA via the RNA obtained in the step 4) and the solid-phase primer to obtain a cDNA-RNA complex, 6) degrading the RNA of the complex obtained in step 5), 7) synthesizing a double-stranded DNA via the cDNA obtained in the step 6) and the liquid-phase primer, and 8) quantifying the double-stranded DNAs obtained in the steps 3) and 7). These steps can be performed in a single reaction solution.
摘要翻译： 本发明提供一种方法和试剂盒，其能够在样品中的痕量RNA中简单快速地检测或定量靶RNA，例如当检测到一种或多种病原微生物或 量化 [解决方法]该方法包括以下步骤：1）使用具有启动子序列和逆转录酶的液相引物合成来自含有靶RNA的样品的cDNA，得到cDNA-RNA复合物，2）降解 复合物，3）通过步骤2）和固相引物获得的cDNA合成双链DNA，4）从双链DNA合成RNA，5）通过步骤4）得到的RNA合成cDNA， 和固相引物以获得cDNA-RNA复合物，6）降解步骤5）中获得的复合物的RNA，7）通过步骤6）中得到的cDNA合成双链DNA和液相 引物，和8）定量在步骤3）和7）中获得的双链DNA。 这些步骤可以在单个反应溶液中进行。
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9.发明申请Optical compensatory sheet producing method and apparatus, thermal treating method and apparatus, and dust removing method and apparatus 审中-公开光学补偿片材的制造方法和装置，热处理方法和装置以及除尘方法和装置公开(公告)号：US20050018116A1
公开(公告)日：2005-01-27
申请号：US10919377
申请日：2004-08-17
申请人： Seiji Ishizuka , Munehiro Ura , Tadashi Sugiyama , Kenji Nakajima , Kazuhiko Fujiwara , Naoyuki Kawanishi
发明人： Seiji Ishizuka , Munehiro Ura , Tadashi Sugiyama , Kenji Nakajima , Kazuhiko Fujiwara , Naoyuki Kawanishi
IPC分类号： G02B5/30 , G02F1/13363 , A61F13/15 , A61F13/20 , G02F1/1335
CPC分类号： B05D3/0413 , B05D7/04 , G02B5/3016 , G02B5/3083 , G02F1/13363 , G02F2001/133633
摘要： An optical compensatory sheet producing apparatus produces an optical compensatory sheet having a liquid crystal layer. In the apparatus, a rubbing unit is supplied with resin film having a first layer including resin, and subjects the first layer to a rubbing process, so as to form an orientation layer. A dust remover removes dust from the orientation layer by use of liquid such as perfluorocarbon, to which the orientation layer is insoluble. A liquid crystal layer coater is disposed downstream from the dust remover, for coating the orientation layer with coating liquid including liquid crystalline compound, so that the liquid crystal layer is formed.
摘要翻译： 光学补偿片材生产设备产生具有液晶层的光学补偿片材。 在该装置中，向具有包括树脂的第一层的树脂膜供给摩擦单元，并且使第一层进行摩擦处理，以形成取向层。 除尘器通过使用取向层不溶于其的全氟化碳等液体从取向层除去灰尘。 在除尘器的下游设置液晶层涂布机，用包含液晶化合物的涂布液涂布取向层，形成液晶层。
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10.发明授权Emulsification and dispersion method of hydrophobic, photographically useful compound 失效疏水性，摄影有用化合物的乳化和分散方法
公开(公告)号：US5817450A
公开(公告)日：1998-10-06
申请号：US603916
申请日：1996-02-22
摘要： An emulsification and dispersion method of a hydrophobic, photographically useful compound comprising dispersing a water-insoluble phase containing the hydrophobic, photographically useful compound in water or in a hydrophilic colloid composition, wherein the water-insoluble phase at a temperature at which the prepared dispersion is stored may have a viscosity of from 100 poise or more to less than 1,000,000 poise at the shear rate of 10 sec.sup.-1. According to the present invention, a silver halide photographic material comprising a fine dispersion of a hydrophobic, photographically useful compound and having an excellent production suitability can be obtained.
摘要翻译： 一种疏水的，照相有用的化合物的乳化和分散方法，包括将含有疏水的，照相有用的化合物的水不溶性相分散在水或亲水胶体组合物中，其中所述水不溶性相在制备的分散体的温度 储存的粘度可以在10秒-1的剪切速率下具有100泊或更多至小于1,000,000泊的粘度。 根据本发明，可以获得包含疏水性，摄影有用的化合物的细分散体并且具有优异的生产适应性的卤化银照相材料。
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