公开(公告)号：US20120295304A1

公开(公告)日：2012-11-22

申请号：US13528126

申请日：2012-06-20

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/02

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

公开(公告)号：US20090191600A1

公开(公告)日：2009-07-30

申请号：US11970203

申请日：2008-01-07

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P13/22

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.

摘要翻译： 制备α-L-天冬氨酰基-L-苯丙氨酸-β-酯（也称为α-L-（β-邻位取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L-天冬氨酰基 提供了L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;商品名：阿斯巴甜），容易地以高产率和低成本地经历复合合成方法。 此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易，便宜且高产量的制备方法。 使用具有催化反应能力的酶或含酶物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸生产α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯，其中 L-苯丙氨酸不对L-天冬氨酸-α，β-二酯的β-酯位点进行亲核攻击，但对其α-酯位点进行亲核攻击。

公开(公告)号：US20120301918A1

公开(公告)日：2012-11-29

申请号：US13528162

申请日：2012-06-20

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/02

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

公开(公告)号：US20120003692A1

公开(公告)日：2012-01-05

申请号：US13198075

申请日：2011-08-04

申请人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

发明人： Kenzo YOKOZEKI ,  Ayako Ohno ,  Seiichi Hara ,  Isao Abe

IPC分类号： C12P21/02

CPC分类号： C07K5/0613 ,  A23L27/32 ,  C07K5/06113 ,  C12P21/02 ,  C12P21/06

摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

摘要翻译： 通过从L-天冬氨酸-α，二酯和L形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 - 苯丙氨酸，其使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α，α-二酯的α-酯位点的酶或酶的物质。

公开(公告)号：US20080171357A1

公开(公告)日：2008-07-17

申请号：US11940555

申请日：2007-11-15

申请人： Kenzo YOKOZEKI ,  Sonoko SUZUKI ,  Seiichi HARA ,  Isao ABE

发明人： Kenzo YOKOZEKI ,  Sonoko SUZUKI ,  Seiichi HARA ,  Isao ABE

IPC分类号： C12P21/00

CPC分类号： C07K5/06 ,  C12N9/16 ,  C12N9/52 ,  C12N9/80 ,  C12P21/02

摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

摘要翻译： 公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。 提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。 该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。