Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester
    2.
    发明授权
    Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权
    制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法

    公开(公告)号:US07745172B2

    公开(公告)日:2010-06-29

    申请号:US11970203

    申请日:2008-01-07

    IPC分类号: C12P21/06

    摘要: A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.

    摘要翻译: 制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯(也称为α-L- - (取代的天冬氨酰基)-L-苯丙氨酸)的方法,其为α-L 提供了天竺葵酰-L-苯丙氨酸-α-甲酯(也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;产品名称:阿斯巴甜),容易地以高产率和低成本而不经过复合合成方法。 此外,提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易且廉价且高产率的制备方法。 使用具有催化反应能力的酶或酶的物质由L-天冬氨酸-α,β-二酯和L-苯丙氨酸制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯 其中L-苯丙氨酸对L-天冬氨酸-α,bgr-siester的“酯”位点不发生亲核攻击,但对其α-酯位点进行亲核攻击。

    Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester
    3.
    发明授权
    Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权
    生产α-L-天冬氨酰-L-苯丙氨酸-β-酯的方法及其制备α-天冬氨酰-L-苯丙氨酸-α-甲酯的方法

    公开(公告)号:US07361458B2

    公开(公告)日:2008-04-22

    申请号:US10876673

    申请日:2004-06-28

    IPC分类号: C12P21/06 C12Q1/00 C12Q1/68

    摘要: A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.

    摘要翻译: 制备α-L-天冬氨酰基-L-苯丙氨酸-β-酯(也称为α-L-(β-邻位取代的天冬氨酰基)-L-苯丙氨酸)的方法,其为α-L-天冬氨酰基 提供了L-苯丙氨酸-α-甲酯(也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;商品名:阿斯巴甜),容易地以高产率和低成本地经历复合合成方法。 此外,提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易,便宜且高产量的制备方法。 使用具有催化反应能力的酶或含酶物质由L-天冬氨酸-α,β-二酯和L-苯丙氨酸生产α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯,其中 L-苯丙氨酸不对L-天冬氨酸-α,β-二酯的β-酯位点进行亲核攻击,但对其α-酯位点进行亲核攻击。

    METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER
    10.
    发明申请
    METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER 有权
    生产ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER的方法及生产ALPHA-L-ASPARTYL-L-苯乙胺 - 阿魏酸酯的方法

    公开(公告)号:US20090191600A1

    公开(公告)日:2009-07-30

    申请号:US11970203

    申请日:2008-01-07

    IPC分类号: C12P13/22

    摘要: A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.

    摘要翻译: 制备α-L-天冬氨酰基-L-苯丙氨酸-β-酯(也称为α-L-(β-邻位取代的天冬氨酰基)-L-苯丙氨酸)的方法,其为α-L-天冬氨酰基 提供了L-苯丙氨酸-α-甲酯(也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;商品名:阿斯巴甜),容易地以高产率和低成本地经历复合合成方法。 此外,提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易,便宜且高产量的制备方法。 使用具有催化反应能力的酶或含酶物质由L-天冬氨酸-α,β-二酯和L-苯丙氨酸生产α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯,其中 L-苯丙氨酸不对L-天冬氨酸-α,β-二酯的β-酯位点进行亲核攻击,但对其α-酯位点进行亲核攻击。