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公开(公告)号：US20110257019A1
公开(公告)日：2011-10-20
申请号：US13172668
申请日：2011-06-29
CPC分类号： C12Q1/6846 , C12Q1/6837 , C12Q2565/537 , C12Q2547/107 , C12Q2537/143
摘要： The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.
摘要翻译： 本发明提供寡核苷酸引物对的微阵列，特别是包含至少一个可切割键的引物的微阵列。 还提供了从一个或多个微阵列捕获寡核苷酸引物对的方法，以及使用捕获的寡核苷酸引物对的方法，例如扩增靶多核苷酸序列。 此外，提供了使用微阵列分离，纯化和/或扩增靶多核苷酸的方法。
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公开(公告)号：US20090280540A1
公开(公告)日：2009-11-12
申请号：US12504485
申请日：2009-07-16
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , C12Q1/6837 , C12Q2565/537 , C12Q2547/107 , C12Q2537/143
摘要： The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.
摘要翻译： 本发明提供寡核苷酸引物对的微阵列，特别是包含至少一个可切割键的引物的微阵列。 还提供了从一个或多个微阵列捕获寡核苷酸引物对的方法，以及使用捕获的寡核苷酸引物对的方法，例如扩增靶多核苷酸序列。 此外，提供了使用微阵列分离，纯化和/或扩增靶多核苷酸的方法。
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公开(公告)号：US20100297628A1
公开(公告)日：2010-11-25
申请号：US12632740
申请日：2009-12-07
CPC分类号： C12Q1/6869 , C12Q2525/113
摘要： The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
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4.发明申请METHODS FOR PRODUCING A PAIRED TAG FROM A NUCLEIC ACID SEQUENCE AND METHODS OF USE THEREOF 有权从核酸序列生产成对标签的方法及其使用方法公开(公告)号：US20100028888A1
公开(公告)日：2010-02-04
申请号：US12468818
申请日：2009-05-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/6816 , C12Q2525/191 , C12Q2521/313 , C12Q2525/131 , C12Q2521/501
摘要： Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5′ end tag and 3′ end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence. In a further embodiment, a method is provided for identifying nucleic acid sequences that encode at least two interacting proteins.
摘要翻译： 提供了从核酸序列产生配对标签的方法，其中配对标签包含核酸序列的5'末端标签和3'末端标签。 在一个实施方案中，核酸序列包含限制性内切核酸酶特异性的两个限制性内切核酸酶识别位点，其将核酸序列向远端切割至限制性内切核酸酶识别位点。 在另一个实施方案中，核酸序列还包含限制性内切核酸酶识别位点，其特异于稀有切割限制性内切核酸酶。 还提供了使用配对标签的方法。 在一个实施方案中，配对标签用于表征核酸序列。 在一个具体实施方案中，核酸序列是基因组。 在一个实施方案中，核酸序列的表征是核型分析。 或者，在另一个实施方案中，核酸序列的表征是该序列的映射。 在另一个实施方案中，提供了用于鉴定编码至少两种相互作用蛋白质的核酸序列的方法。
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5.发明授权Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof 有权从核酸序列产生配对标签的方法及其使用方法公开(公告)号：US09309560B2
公开(公告)日：2016-04-12
申请号：US12468818
申请日：2009-05-19
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/6816 , C12Q2525/191 , C12Q2521/313 , C12Q2525/131 , C12Q2521/501
摘要： Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5′ end tag and 3′ end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence. In a further embodiment, a method is provided for identifying nucleic acid sequences that encode at least two interacting proteins.
摘要翻译： 提供了从核酸序列产生配对标签的方法，其中配对标签包含核酸序列的5'末端标签和3'末端标签。 在一个实施方案中，核酸序列包含限制性内切核酸酶特异性的两个限制性内切核酸酶识别位点，其将核酸序列向远端切割至限制性内切核酸酶识别位点。 在另一个实施方案中，核酸序列还包含限制性内切核酸酶识别位点，其特异于稀有切割限制性内切核酸酶。 还提供了使用配对标签的方法。 在一个实施方案中，配对标签用于表征核酸序列。 在一个具体实施方案中，核酸序列是基因组。 在一个实施方案中，核酸序列的表征是核型分析。 或者，在另一个实施方案中，核酸序列的表征是序列的映射。 在另一个实施方案中，提供了用于鉴定编码至少两种相互作用蛋白质的核酸序列的方法。
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公开(公告)号：US20100298551A1
公开(公告)日：2010-11-25
申请号：US12632730
申请日：2009-12-07
IPC分类号： C07H21/04
CPC分类号： C12Q1/6869 , C12Q2525/113
摘要： The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
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公开(公告)号：US07645866B2
公开(公告)日：2010-01-12
申请号：US11476423
申请日：2006-06-28
CPC分类号： C12Q1/6869 , C12Q2525/113
摘要： The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
摘要翻译： 本发明包括用于产生包含（例如一个或多个）硫代硫醇键的修饰的多核苷酸序列的方法，用于测定包含（例如一个或多个）硫代硫醇键的多核苷酸序列的方法，以及用于分离正向和反向延伸的方法 包括（例如，一个或多个）硫代硫醇键的产物。 本发明还包括用于产生和/或确定包含（例如一个或多个）硫代磷酸酯键的修饰多核苷酸序列的试剂盒。
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8.发明申请Directed assembly of amplicons to enhance read pairing signature with massively parallel short read sequencers 审中-公开扩展子的定向组装，以增强大量并行短读序列的读配对签名公开(公告)号：US20090036325A1
公开(公告)日：2009-02-05
申请号：US12154294
申请日：2008-05-21
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q1/6869 , C12Q2533/107 , C12Q2531/113 , C12Q2523/101
摘要： The present teachings relate to improved methods, kits, and compositions for making nucleic acid libraries and sequencing nucleic acids. In some embodiments, directionally defined concatamers are generated, facilitating sequencing efforts.
摘要翻译： 本教导涉及用于制备核酸文库和测序核酸的改进的方法，试剂盒和组合物。 在一些实施例中，生成定向定义的连接器，有利于测序工作。
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公开(公告)号：US07993842B2
公开(公告)日：2011-08-09
申请号：US12504485
申请日：2009-07-16
申请人： Kevin McKernan , Alan Blanchard , Douglas R. Smith
发明人： Kevin McKernan , Alan Blanchard , Douglas R. Smith
CPC分类号： C12Q1/6846 , C12Q1/6837 , C12Q2565/537 , C12Q2547/107 , C12Q2537/143
摘要： The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.
摘要翻译： 本发明提供寡核苷酸引物对的微阵列，特别是包含至少一个可切割键的引物的微阵列。 还提供了从一个或多个微阵列捕获寡核苷酸引物对的方法，以及使用捕获的寡核苷酸引物对的方法，例如扩增靶多核苷酸序列。 此外，提供了使用微阵列分离，纯化和/或扩增靶多核苷酸的方法。
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公开(公告)号：US20100285461A1
公开(公告)日：2010-11-11
申请号：US12632713
申请日：2009-12-07
申请人： Douglas R. Smith , Kevin McKernan
发明人： Douglas R. Smith , Kevin McKernan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2525/113
摘要： The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
摘要翻译： 本发明包括用于产生包含（例如一个或多个）硫代硫醇键的修饰的多核苷酸序列的方法，用于测定包含（例如一个或多个）硫代硫醇键的多核苷酸序列的方法，以及用于分离正向和反向延伸的方法 包括（例如，一个或多个）硫代硫醇键的产物。 本发明还包括用于产生和/或确定包含（例如一个或多个）硫代磷酸酯键的修饰多核苷酸序列的试剂盒。
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