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    Toxin genes and methods for their use
    3.
    发明授权
    Toxin genes and methods for their use 有权
    毒素基因及其使用方法

    公开(公告)号：US08461421B2

    公开(公告)日：2013-06-11

    申请号：US12491396

    申请日：2009-06-25

    申请人： Kimberly S. Sampson ,  Daniel John Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

    发明人： Kimberly S. Sampson ,  Daniel John Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

    IPC分类号： A01H5/00 ,  C12N15/31 ,  C12N15/82 ,  A01N37/18 ,  C07K14/325 ,  C12P21/02 ,  C12N1/21

    CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

    摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：61-121和133-141所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-60,124所示的核苷酸序列 -132和142-283，以及其变体和片段。

    TOXIN GENES AND METHODS FOR THEIR USE
    4.
    发明申请
    TOXIN GENES AND METHODS FOR THEIR USE 有权
    毒素基因及其使用方法

    公开(公告)号：US20100005543A1

    公开(公告)日：2010-01-07

    申请号：US12491396

    申请日：2009-06-25

    申请人： Kimberly S. Sampson ,  Daniel John Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

    发明人： Kimberly S. Sampson ,  Daniel John Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

    IPC分类号： C12N15/82 ,  C12N15/32 ,  C12N1/21 ,  C07K14/325 ,  C07K16/12 ,  A01N37/18 ,  C12P21/02 ,  A01H5/00 ,  C12N5/10 ,  A01H5/10 ,  A01P7/04

    CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

    摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：61-121和133-141所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-60,124所示的核苷酸序列 -132和142-283，以及其变体和片段。