公开(公告)号：US10030065B2

公开(公告)日：2018-07-24

申请号：US12644554

申请日：2009-12-22

申请人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jørgen Schøller ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

发明人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jørgen Schøller ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

IPC分类号： C07K14/74 ,  A61K47/61 ,  A61K47/64 ,  A61K47/66 ,  B82Y5/00 ,  A61K38/00

摘要： The present invention relates to a soluble negative control MHC multimer comprising a nonsense peptide that binds the MHC protein efficiently, but that does not support binding of the resultant MHC-peptide complex to the desired T Cell Receptor. The nonsense peptide is designed to i) have a length enabling binding to the MHC allele in question, ii) have appropriate amino acids at relevant anchor positions which anchor the nonsense peptide to the peptide-binding groove of the MHC, iii) have amino acids outside the anchor positions that do not support binding to a T Cell Receptor, and iv) have an amino acid sequence that is different from the linear sequence of any naturally occurring peptide.

公开(公告)号：US20100248257A1

公开(公告)日：2010-09-30

申请号：US12647747

申请日：2009-12-28

申请人： Kivin Jacobsen ,  Henrik Pedersen ,  Liselotte Brix ,  Jesper Lohse ,  Jørgen Schøller ,  Tina Jakobsen

发明人： Kivin Jacobsen ,  Henrik Pedersen ,  Liselotte Brix ,  Jesper Lohse ,  Jørgen Schøller ,  Tina Jakobsen

IPC分类号： G01N33/53

CPC分类号： C07K14/70539 ,  A61K38/00 ,  A61K47/61 ,  A61K47/6425 ,  A61K47/665 ,  B82Y5/00

摘要： The present invention relates to the fields of analysis, diagnostics, prognostics, standardization, characterization and enumeration of entities such as biological cells, as well as therapeutical applications. Accordingly, the present invention in preferred aspects is directed to methods for the detection and/or analysis and/or isolation and optionally further manipulation of entities, such as cells, particles, and supra-molecular structures.

摘要翻译： 本发明涉及诸如生物细胞的实体以及治疗应用的分析，诊断，预测，标准化，表征和计数的领域。 因此，本发明在优选方面涉及用于检测和/或分析和/或分离和任选地进一步操纵诸如细胞，颗粒和超分子结构的实体的方法。

公开(公告)号：US20100168390A1

公开(公告)日：2010-07-01

申请号：US12644554

申请日：2009-12-22

申请人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jorgen Scholler ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

发明人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jorgen Scholler ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

IPC分类号： C07K14/74

CPC分类号： C07K14/70539 ,  A61K38/00 ,  A61K47/61 ,  A61K47/6425 ,  A61K47/665 ,  B82Y5/00 ,  Y02A50/55 ,  Y02A50/57 ,  Y02A50/58

摘要： The present invention describes novel methods to generate MHC multimers and methods to improve existing and new MHC multimers. The invention also describes improved methods for the use of MHC multimers in analysis of T-cells in samples including diagnostic and prognostic methods. Furthermore the use of MHC multimers in therapy are described, e.g. anti-tumour and anti-virus therapy, including isolation of antigen specific T-cells capable of inactivation or elimination of undesirable targeT-cells or isolation of specific T-cells capable of regulation of other immune cells.

摘要翻译： 本发明描述了生成MHC多聚体的新方法和改进现有的和新的MHC多聚体的方法。 本发明还描述了在样品中T细胞分析中使用MHC多聚体的改进方法，包括诊断和预后方法。 此外，MHC多聚体在治疗中的使用描述如下。 抗肿瘤和抗病毒治疗，包括分离能够灭活或消除不需要的targeT细胞的抗原特异性T细胞或分离能够调节其他免疫细胞的特异性T细胞。

公开(公告)号：US20090061478A1

公开(公告)日：2009-03-05

申请号：US12161579

申请日：2007-01-30

申请人： Lene Have Poulsen ,  Kivin Jacobsen ,  Ian Storie ,  Jesper Laursen

发明人： Lene Have Poulsen ,  Kivin Jacobsen ,  Ian Storie ,  Jesper Laursen

IPC分类号： C12Q1/06

CPC分类号： G01N33/505 ,  G01N2015/0065 ,  G01N2015/1486

摘要： The present invention discloses novel methods and compositions for identifying particular cell types in whole blood samples and defining either the concentration or ‘absolute count’ of these cells per unit volume of the sample. More specifically, the invention relates to methods for quantifying the antigen-specific T cells or defining the relative percentage of said cells in un-lysed whole blood. Further, the invention relates to kits for the preparation of whole blood samples for high-speed quantification of particular cell types, e.g. antigen specific T-cells, in said whole blood samples by flow cytometry.

摘要翻译： 本发明公开了用于鉴定全血样品中特定细胞类型的新颖方法和组合物，并定义了每单位体积样品中这些细胞的浓度或“绝对计数”。 更具体地，本发明涉及定量抗原特异性T细胞或限定未裂解全血中所述细胞的相对百分比的方法。 此外，本发明涉及用于制备用于高速定量特定细胞类型的全血样品的试剂盒，例如， 抗原特异性T细胞，通过流式细胞仪检测所有全血样品。