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    Mammalian common lymphoid progenitor cell
    2.
    发明授权
    Mammalian common lymphoid progenitor cell 有权
    哺乳动物常见淋巴祖细胞

    公开(公告)号：US07297329B2

    公开(公告)日：2007-11-20

    申请号：US11093951

    申请日：2005-03-29

    申请人： Koichi Akashi ,  Irving L. Weissman ,  Motonari Kondo

    发明人： Koichi Akashi ,  Irving L. Weissman ,  Motonari Kondo

    IPC分类号： A01N65/00

    CPC分类号： C12N5/0635 ,  A61K38/00 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2510/00

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for lymphoid lineages, but lacking the potential to differentiate into myeloid and erythroid lineages. Methods are provided for the isolation and culture of this common lymphoid progenitor cell (CLP). The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor α); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. The murine cells are also characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are predominantly cycling, blast cells. These cells give rise to B cells, T cells and natural killer cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于淋巴谱系的祖细胞活性，但缺乏分化为骨髓和红系谱系的潜力。 提供了用于分离和培养这种常见淋巴祖细胞（CLP）的方法。 细胞富集方法使用特异性识别CDw127（IL-7受体α）的试剂; CD117（c-kit）蛋白，与在谱系确定细胞上表达的其他标志物结合。 鼠细胞也被表征为表达低水平的sca-1（Ly-6E和Ly-6A）。 CLP主要是循环，胚细胞。 这些细胞产生B细胞，T细胞和自然杀伤细胞，这通过其体外和体内的生长和分化证明。

    Mammalian myeloid progenitor cell subsets
    3.
    发明授权
    Mammalian myeloid progenitor cell subsets 有权
    哺乳动物骨髓祖细胞亚群

    公开(公告)号：US07618654B2

    公开(公告)日：2009-11-17

    申请号：US11874841

    申请日：2007-10-18

    申请人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    发明人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    IPC分类号： A61K35/28 ,  G01N33/53

    CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2503/00 ,  C12Q1/6881 ,  C12Q2600/158

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. Tηε χελλ ενιχημεντ μετηoδσ εμπλoψεαγεντσ τηατ σπεχαλλψεψoγνιζε Tηψ-1; ανδIΛ-7 Pα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于骨髓谱系的祖细胞活性，但缺乏分化成淋巴谱系的潜力。 该群体进一步分为特定的骨髓祖细胞亚群，包括常见的髓系祖细胞（CMP），巨核细胞/红细胞祖细胞（MEP）和粒细胞/单核细胞谱系祖细胞（GMP）。 提供了用于分离和培养这些亚群体的方法。 CMP群体产生所有骨髓谱系，并且可以产生专门致力于红细胞/巨核细胞或骨髓单核细胞谱系的两个另外的和可分离的祖细胞群体。 “=”2.46mm“wi =”1.78mm“file =”US07618654-20091117-P00001.TIF“alt =”自定义字符“img-content =”character“ img-format =“tif”/> iotachietamuepsilonnutau muepsilontauetaodeltasigma epsilonmupilambdaopsi epsilonalphagammaepsilonnutausigma tauetaalphatau sigmapiepsilonchialphalambdalambdapsi epsilonpsiogammanuiotazetaepsilon Tetapsi-1; alphanudeltaILambda-7 Palpha，与在谱系确定的细胞上表达的其他标志物结合。 这些细胞产生各种骨髓细胞，包括巨核细胞，粒细胞，树突状细胞和红细胞，其体外和体内的生长和分化证明。

    Mammalian myeloid progenitor cell subsets
    5.
    发明授权
    Mammalian myeloid progenitor cell subsets 有权
    哺乳动物骨髓祖细胞亚群

    公开(公告)号：US07300760B2

    公开(公告)日：2007-11-27

    申请号：US10737576

    申请日：2003-12-15

    申请人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    发明人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    IPC分类号： G01N33/53 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2503/00 ,  C12Q1/6881 ,  C12Q2600/158

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. The cell enrichment methods employ reagents that specifically recognize Thy-1; and IL-7Rα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于骨髓谱系的祖细胞活性，但缺乏分化成淋巴谱系的潜力。 该群体进一步分为特定的骨髓祖细胞亚群，包括常见的髓系祖细胞（CMP），巨核细胞/红细胞祖细胞（MEP）和粒细胞/单核细胞谱系祖细胞（GMP）。 提供了用于分离和培养这些亚群体的方法。 CMP群体产生所有骨髓谱系，并且可以产生专门致力于红细胞/巨核细胞或骨髓单核细胞谱系的两个另外的和可分离的祖细胞群体。 细胞富集方法采用特异性识别Thy-1的试剂; 和IL-7Rα，与在谱系承诺细胞上表达的其他标志物结合。 这些细胞产生各种骨髓细胞，包括巨核细胞，粒细胞，树突状细胞和红细胞，其体外和体内的生长和分化证明。

    MAMMALIAN MYELOID PROGENITOR CELL SUBSETS
    6.
    发明申请
    MAMMALIAN MYELOID PROGENITOR CELL SUBSETS 有权

    公开(公告)号：US20080131408A1

    公开(公告)日：2008-06-05

    申请号：US11874841

    申请日：2007-10-18

    申请人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    发明人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi ,  Markus Gabriel Manz ,  Toshihiro Miyamoto

    IPC分类号： A01N63/00 ,  C12N5/00

    CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2503/00 ,  C12Q1/6881 ,  C12Q2600/158

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. Tηε χελλ ενιχημεντ μετηoδσ εμπλoψεαγεντσ τηατ σπεχαλλψεψoγνιζε Tηψ-1; ανδIΛ-7 Pα, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于骨髓谱系的祖细胞活性，但缺乏分化成淋巴谱系的潜力。 该群体进一步分为特定的骨髓祖细胞亚群，包括常见的髓系祖细胞（CMP），巨核细胞/红细胞祖细胞（MEP）和粒细胞/单核细胞谱系祖细胞（GMP）。 提供了用于分离和培养这些亚群体的方法。 CMP群体产生所有骨髓谱系，并且可以产生专门致力于红细胞/巨核细胞或骨髓单核细胞谱系的两个另外的和可分离的祖细胞群体。 “=”2.46mm“wi =”1.78mm“file =”US20080131408A1-20080605-P00001.TIF“img-content =”character“img-format =”tif“=”CUSTOM-CHARACTER-00001“ “/> iotachietamuepsilonnutau muepsilontauetaodeltasigma epsilonmupilambdaopsi epsilonalphagammaepsilonnutphaigma tauetaalphatau sigmapiepsilonchialphalambdalambdapsi epsilonpsiogammanuiotazetaepsilon Tetapsi-1; alphanudeltaILambda-7 Palpha，与在谱系确定的细胞上表达的其他标志物结合。 这些细胞产生各种骨髓细胞，包括巨核细胞，粒细胞，树突状细胞和红细胞，其体外和体内的生长和分化证明。

    Mammalian myeloid progenitor cell subsets
    7.
    发明授权
    Mammalian myeloid progenitor cell subsets 有权
    哺乳动物骨髓祖细胞亚群

    公开(公告)号：US06465247B1

    公开(公告)日：2002-10-15

    申请号：US09607529

    申请日：2000-06-29

    申请人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi

    发明人： Irving L. Weissman ,  David Jeffrey Traver ,  Koichi Akashi

    IPC分类号： C12N506

    CPC分类号： C12Q1/6881 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2503/00 ,  C12Q2600/158

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor &agr;); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于骨髓谱系的祖细胞活性，但缺乏分化成淋巴谱系的潜力。 该群体进一步分为特定的骨髓祖细胞亚群，包括常见的髓系祖细胞（CMP），巨核细胞/红细胞祖细胞（MEP）和粒细胞/单核细胞谱系祖细胞（GMP）。 提供了用于分离和培养这些亚群体的方法。 CMP群体产生所有骨髓谱系，并且可以产生专门致力于红细胞/巨核细胞或骨髓单核细胞谱系的两个另外的和可分离的祖细胞群体。 细胞富集方法使用特异性识别CDw127（IL-7受体α）的试剂; CD117（c-kit）蛋白，与在谱系确定细胞上表达的其他标志物结合。 这些细胞产生各种骨髓细胞，包括巨核细胞，粒细胞，树突状细胞和红细胞，其体外和体内的生长和分化证明。