公开(公告)号：US20080090306A1

公开(公告)日：2008-04-17

申请号：US11862682

申请日：2007-09-27

申请人： Koji KURUMA ,  Toshihide Ezoe

发明人： Koji KURUMA ,  Toshihide Ezoe

IPC分类号： G01N33/543 ,  G01N21/55

CPC分类号： G01N33/54353 ,  G01N21/553 ,  G01N33/54373 ,  G01N33/553

摘要： An object of the present invention is to provide a method for preventing the generation of negative signals because of dissociation of a ligand from a surface and improving the precision of signals indicating binding between a ligand and an analyte. The present invention provides a method for immobilizing a biomolecule on a carrier having reactive groups which comprises: (i) a step of activating some reactive groups so that they can form a covalent bond with a biomolecule; (ii) a step of reacting the biomolecule with the aforementioned activated reactive groups; and (iii) a step of again activating some reactive groups; wherein the steps (i), (ii) and (iii) are performed in this order.

摘要翻译： 本发明的目的是提供一种防止由于配体与表面解离而产生负信号的方法，提高了表示配体与分析物之间结合的信号的精度。 本发明提供了将生物分子固定在具有反应性基团的载体上的方法，该方法包括：（i）使一些反应性基团活化以使其与生物分子形成共价键的步骤; （ii）使生物分子与上述活化的反应性基团反应的步骤; 和（iii）再次激活一些反应性基团的步骤; 其中步骤（i），（ii）和（iii）按此顺序执行。

公开(公告)号：US20090068746A1

公开(公告)日：2009-03-12

申请号：US12204640

申请日：2008-09-04

申请人： Koji KURUMA ,  Toshihide EZOE

发明人： Koji KURUMA ,  Toshihide EZOE

IPC分类号： G01N31/00

CPC分类号： G01N33/54373 ,  Y10T436/10

摘要： It is an object of the present invention to provide a method for measuring the interaction between a physiologically active substance immobilized on the surface of a substrate and a test substance, wherein the influence of negative signals intermittently generated due to desorption of the physiologically active substance as a ligand from the surface of the substrate upon the measured value that indicates the binding amount of a test substance as an analyte can be eliminated. The present invention provides a method for measuring the interaction between a physiologically active substance immobilized on the surface of a substrate and a test substance, wherein a calibration curve is produced by using baseline values obtained by repeatedly measuring a single type of solution, and the measurement value of said test substance is calibrated by said calibration curve, so as to obtain the interaction signals of said test substance.

摘要翻译： 本发明的目的是提供一种测定固定在基材表面上的生理活性物质与试验物质之间的相互作用的方法，其中由于生理活性物质的解吸而间歇产生的负信号的影响为 可以消除表示作为分析物的测试物质的结合量的测量值从基材表面的配体。 本发明提供一种测定固定在基材表面的生理活性物质与检测物质之间的相互作用的方法，其中通过使用通过重复测定单一溶液而获得的基线值来产生校准曲线，并且测量 通过所述校准曲线校准所述测试物质的值，以获得所述测试物质的相互作用信号。

公开(公告)号：US20100278696A1

公开(公告)日：2010-11-04

申请号：US12237498

申请日：2008-09-25

申请人： Koji KURUMA ,  Koichi Minami

发明人： Koji KURUMA ,  Koichi Minami

IPC分类号： G01N33/543 ,  G01N21/17 ,  G01N21/41

CPC分类号： G01N33/54373 ,  C12Q1/002 ,  G01N33/54393

摘要： It is an object of the present invention to provide a biosensor having excellent preservation stability. The present invention provides a biosensor wherein a first hydrophilic polymer layer is immobilized on a self-assembled membrane applied onto the surface of a metal substrate, a second hydrophilic polymer layer is applied onto the first hydrophilic polymer layer, and the total film thickness of the first hydrophilic polymer layer and the second hydrophilic polymer layer in a dry state is between 20 nm and 100 nm.

摘要翻译： 本发明的目的是提供具有优异的保存稳定性的生物传感器。 本发明提供了一种生物传感器，其中将第一亲水性聚合物层固定在施加到金属基材表面上的自组装膜上，将第二亲水性聚合物层施加到第一亲水性聚合物层上， 处于干燥状态的第一亲水性聚合物层和第二亲水性聚合物层在20nm和100nm之间。