公开(公告)号：US20220306993A1

公开(公告)日：2022-09-29

申请号：US17617876

申请日：2020-06-10

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Ryoji HIRAIDE ,  Kenta SUTO

IPC分类号： C12N5/074 ,  C12N5/00

摘要： Provided is a cell culture method including introducing a factor into cells in a cell culture vessel, and culturing the cells into which the factor has been introduced in the same cell culture vessel. Also provided is a mononuclear cell collection method including treating blood to prepare a treated blood from which erythrocytes have been at least partially removed, diluting the treated blood, causing sedimentation of mononuclear cells contained in the diluted treated blood, removing the supernatant from the diluted treated blood, and collecting the mononuclear cells.

公开(公告)号：US20220213446A1

公开(公告)日：2022-07-07

申请号：US17646398

申请日：2021-12-29

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Kenta SUTO

IPC分类号： C12N5/074 ,  C12N5/00

摘要： According to the present disclosure, there is provided a method for culturing cells into which a reprogramming factor is introduced including culturing cells into which a reprogramming factor is introduced; and recovering all cells into which the reprogramming factor is introduced and seeding and passaging at least part of the recovered cells in a medium. In addition, there is provided a method for culturing cells into which a reprogramming factor is introduced, including culturing cells into which a reprogramming factor is introduced; and inducing somatic cells different from pluripotent stem cells without passaging the cells into which the reprogramming factor is introduced.

公开(公告)号：US20220389368A1

公开(公告)日：2022-12-08

申请号：US17817287

申请日：2022-08-03

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Brendan KELLY ,  Kenta SUTO ,  Hidenori SHIMODA ,  Ryoji HIRAIDE

IPC分类号： C12M1/36 ,  C12M1/00 ,  C12M1/34 ,  C12M1/33 ,  C12M1/42 ,  C12N5/078 ,  C12M3/00 ,  C12M1/02 ,  C12M1/12 ,  C12N5/074

摘要： A stem cell production system provided with a preintroduction cell-feeding solution channel 20 through which a solution containing cells passes, an induction factor-feeding solution mechanism 21 for feeding a pluripotency induction factor to the preintroduction cell-feeding solution channel 20, a factor introduction device 30 connected to the preintroduction cell-feeding solution channel 20 for making cells with induction factor introduced by introducing the pluripotency induction factor into the cells, a cell mass-making device 40 for making multiple cell masses comprising stem cells by culturing the cells with induction factor introduced, and a packaging device 100 for sequentially packaging each of the multiple cell masses.

公开(公告)号：US20200040297A1

公开(公告)日：2020-02-06

申请号：US16485146

申请日：2018-02-23

申请人： Koji TANABE ,  I PEACE, INC.

发明人： Koji TANABE ,  Kenta SUTO ,  Ryoji HIRAIDE

IPC分类号： C12M1/34 ,  C12M1/00 ,  C12N5/0735

摘要： This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

公开(公告)号：US20240316117A1

公开(公告)日：2024-09-26

申请号：US18734602

申请日：2024-06-05

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Kenta SUTO

IPC分类号： A61K35/545 ,  A61K8/98 ,  A61P17/14 ,  A61Q19/08

CPC分类号： A61K35/545 ,  A61K8/982 ,  A61P17/14 ,  A61Q19/08

摘要： Provided are a pharmaceutical composition or a pharmaceutical composition raw material, which contains supernatant from a culture medium used during culture to induce pluripotent stem cells.

公开(公告)号：US20230313102A1

公开(公告)日：2023-10-05

申请号：US18157369

申请日：2023-01-20

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Kenta SUTO ,  Ryoji HIRAIDE

IPC分类号： C12M1/00

CPC分类号： C12M23/58 ,  C12M29/00

摘要： The present invention provides a culturing system and method for culturing cells that can culture cells in parallel. The culturing system comprises a cell storage 10 configured to store cells, a plurality of culture apparatuses 20, and a distribution flow path 30 configured to distribute the cells from the cell storage 10 to the culture apparatuses 20, in which each of the culture apparatuses 20 comprises a plurality of culture vessels 21, a supply flow path 22 configured to selectively supply a fluid from the distribution flow path 30 to the culture vessels 21, and a connection flow path 23 configured to connect the culture vessels 21; the culture vessels 21 are connected in series with the connection flow path 23 in each of the culture apparatuses 20; and the supply flow path 22 is configured to selectively supply the fluid to the culture vessels so that the cells are sequentially transferred among the culture vessels 21 through the connection flow path 23 in each of the culture apparatuses 20.

公开(公告)号：US20230002717A1

公开(公告)日：2023-01-05

申请号：US17931036

申请日：2022-09-09

申请人： Koji Tanabe ,  I Peace, Inc.

发明人： Koji TANABE ,  Kenta SUTO ,  Ryoji HIRAIDE

IPC分类号： C12M1/00 ,  A01N1/02 ,  C12M1/26 ,  C12N7/00

摘要： A somatic cell production system comprising a preintroduction cell solution-feeding channel 20 through which a preintroduction cell-containing solution passes, a factor introducing device 30 that is connected to the preintroduction cell solution-feeding channel 20 and introduces a somatic cell inducing factor into preintroduction cells to prepare inducing factor-introduced cells, and a cell preparation device 40 in which the inducing factor-introduced cells are cultured to prepare somatic cells.

公开(公告)号：US20190338237A1

公开(公告)日：2019-11-07

申请号：US16299048

申请日：2019-03-11

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Brendan KELLY ,  Kenta SUTO ,  Hidenori SHIMODA ,  Ryoji HIRAIDE

IPC分类号： C12M1/36 ,  C12M1/42 ,  C12M1/00 ,  C12M1/33 ,  C12N5/074 ,  C12M1/02 ,  C12M3/00 ,  C12N5/078 ,  C12M1/34 ,  C12M1/12

摘要： A cell mass dissociator for a pluripotent stem cell production system.

公开(公告)号：US20230183653A1

公开(公告)日：2023-06-15

申请号：US18064189

申请日：2022-12-09

申请人： Koji Tanabe ,  I Peace, Inc.

发明人： Koji TANABE ,  Kenta SUTO

IPC分类号： C12N5/074 ,  C12N15/86

CPC分类号： C12N5/0696 ,  C12N15/86 ,  C12N2501/23 ,  C12N2500/42 ,  C12N2760/18843

摘要： According to the present disclosure, there is provided a method for producing stem cells including: preparing somatic cells; preparing a chimeric virus including a virus-derived genomic RNA harboring an inducer RNA that induces somatic cells into stem cells and a virus-derived envelope surrounding the genomic RNA, wherein the genomic RNA and the envelope are derived from different viruses; and introducing the inducer RNA into the somatic cells using the chimeric virus.

公开(公告)号：US20230061798A1

公开(公告)日：2023-03-02

申请号：US17759327

申请日：2021-01-22

申请人： Koji TANABE ,  I Peace, Inc.

发明人： Koji TANABE ,  Kenta SUTO

IPC分类号： C12N5/074

摘要： According to the present disclosure, there is provided a method for culturing factor-introduced cells, the method including culturing factor-introduced cells and recovering the factor-introduced cells and seeding at least part of the recovered cells in a medium for seeding. In addition, there is provided a method for culturing factor-introduced cells, the method including culturing factor-introduced cells and inducing the factor-introduced cells to somatic cells different from pluripotent stem cells without passaging.