公开(公告)号：US20220213463A1

公开(公告)日：2022-07-07

申请号：US17292967

申请日：2018-11-28

申请人： KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

发明人： Jung-Hyun LEE ,  Jae Kyu LIM ,  Hyun Sook LEE ,  Sung Gyun KANG ,  Kae Kyoung KWON ,  Yun Jae KIM ,  Ji-in YANG

IPC分类号： C12N9/96 ,  C12N9/02 ,  C12N15/52 ,  C12P7/40

摘要： The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel Thermococcus strain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction. Furthermore, it is possible to ensure the stability of each enzyme by allowing the enzymes to exist in a physically fixed state in cells.

公开(公告)号：US20150132819A1

公开(公告)日：2015-05-14

申请号：US14480332

申请日：2014-09-08

申请人： Korea Institute of Ocean Science & Technology

发明人： Sung Gyun KANG ,  Jung Hyun LEE ,  Hyun Sook LEE ,  Kae Kyoung KWON ,  Tae Wan KIM ,  Yun Jae KIM ,  Min Sik KIM ,  Seong Hyuk LEE ,  Seung Seob BAE ,  Ae Ran CHOI

IPC分类号： C12P3/00 ,  C12N15/67

CPC分类号： C12P3/00 ,  C07K14/195 ,  C12N1/20 ,  C12N15/67

摘要： The present invention provides Thermococcus onnurineus MC02 strain (accession no. KCTC12511BP) having increased hydrogen production ability, wherein the expression of rchA gene of the strain increases. Also, the present invention provides a method for producing the strain belonged to the genus Thermococcus having increased hydrogen production ability comprising, increasing the expression of rchA gene of the strain, and hydrogen production method using the strain.

摘要翻译： 本发明提供了具有增加的氢产生能力的Thermococcus onnurineus MC02菌株（登录号KCTC12511BP），其中菌株rchA基因的表达增加。 此外，本发明提供了一种生产属于Thermococcus属的属于具有增加的生产氢能力的菌株的方法，其包括增加菌株的rchA基因的表达和使用该菌株的氢生产方法。

公开(公告)号：US20140295519A1

公开(公告)日：2014-10-02

申请号：US14251713

申请日：2014-04-14

申请人： KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

发明人： Sung Gyun KANG ,  Jung Hyun LEE ,  Kae Kyoung KWON ,  Hyun Sook LEE ,  Tae Wan KIM ,  Yun Jae KIM ,  Min Sik KIM ,  Jeong Ho JEON

IPC分类号： C12P3/00

CPC分类号： C12P3/00 ,  C12N9/0067 ,  C12Y112/98001

摘要： The present invention relates to a Thermococcus onnurineus NA1 mutant having an increased ability to produce hydrogen from formate and a method of producing hydrogen the same. The Thermococcus onnurineus NA1 mutant according to the invention has an increased ability to produce hydrogen in a formate-containing medium compared to wild-type Thermococcus onnurineus NA1 and shows an increase in growth rate compared to the wild-type. The use of the mutant strain according to the invention can produce hydrogen with high efficiency from formate.

摘要翻译： 本发明涉及具有增加从甲酸生成氢的能力的嗜热热球菌NA1突变体及其生产氢的方法。 与野生型Thermococcus onnurineus NA1相比，根据本发明的Thermococcus onnurineus NA1突变体在含甲酸盐的培养基中产生氢的能力增加，并且与野生型相比显示生长速率的增加。 根据本发明的突变菌株的使用可以从甲酸盐以高效率生产氢。