公开(公告)号：US20160168580A1

公开(公告)日：2016-06-16

申请号：US14860373

申请日：2015-09-21

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jun Won CHWA ,  Youngsoon UM ,  Gyeong Taek GONG ,  Yunje KIM

IPC: C12N15/74

CPC classification number: C12N15/74

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

Abstract translation: 本公开公开了可以用于蓝细菌和大肠杆菌的载体，其包含顺序地作为复制起点的pUC复制起点; 壮观霉素抗性基因作为选择标记; 和选自trc启动子，tetA启动子或修饰的tetA启动子，BAD启动子和cbbL启动子的启动子。 可以使用用载体转化的宿主细胞有效地产生工业上有用的物质。 此外，载体可以用于通过简单组合插入各种靶基因，结果可以有效地制备各种载体。

公开(公告)号：US20160257966A1

公开(公告)日：2016-09-08

申请号：US15057551

申请日：2016-03-01

Applicant: Korea Institute of Science and Technology

Inventor： Han Min WOO ,  Youngsoon UM ,  Jun Won CHWA ,  Gyeongtaek GONG

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12N9/88 ,  C12N9/13 ,  C12N9/93 ,  C12Y208/03009 ,  C12Y401/01004 ,  C12Y602/01001 ,  Y02P60/247

Abstract: Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

Abstract translation: 本文公开了具有改进的丙酮生产能力的转化的聚球藻细菌菌株和用于生产丙酮的方法，以及使用该方法除去二氧化碳的方法。 在一方面，本公开的转化的细小球藻菌株可以使用二氧化碳作为碳源以高选择性产生丙酮。 本公开是经济的，因为细菌聚球藻菌株可以使用存在于大气中的二氧化碳作为碳源经济地生产高附加值的丙酮，而不需要额外的催化反应。 此外，本公开是环境友好的，因为可以使用微生物除去或减少大气中的二氧化碳。