公开(公告)号：US20100273259A1

公开(公告)日：2010-10-28

申请号：US12765472

申请日：2010-04-22

申请人： Krishanu Saha ,  Ying Mei ,  Said R. Bogatyrev ,  Daniel G. Anderson ,  Rudolf Jaenisch ,  Robert S. Langer ,  Morgan Alexander ,  Martyn Davies ,  Jing Yang ,  Christian J. Kastrup ,  Andrew Urquhart

发明人： Krishanu Saha ,  Ying Mei ,  Said R. Bogatyrev ,  Daniel G. Anderson ,  Rudolf Jaenisch ,  Robert S. Langer ,  Morgan Alexander ,  Martyn Davies ,  Jing Yang ,  Christian J. Kastrup ,  Andrew Urquhart

IPC分类号： C12N5/0797

CPC分类号： G01N23/2258 ,  C12N5/0068 ,  C12N2535/10 ,  H01J49/00

摘要： The present disclosure provides a device and a cell culture system comprising a substrate that generates significant chemical ion signatures adapted for culturing stem cells. This disclosure further provides unique surface properties, such as surface wettability, along with defined polymer microspot environments in an array, for effectively supporting the propagation and differentiation of human pluripotent stem cells in vitro. Methods of culturing, maintenance, differentiating stem cells as well as reprogramming somatic cells into stem cells using the device and the cell culture system with the suitable substrates, along with suitable culture media, are also provided.

摘要翻译： 本公开提供了一种装置和细胞培养系统，其包含生成适于培养干细胞的显着化学离子特征的底物。 本公开进一步提供独特的表面性质，例如表面润湿性，以及阵列中限定的聚合物微球环境，用于在体外有效支持人多能干细胞的繁殖和分化。 还提供了使用装置和具有合适底物的细胞培养系统以及合适的培养基将培养，维持，分化干细胞以及将体细胞重编程成干细胞的方法。

公开(公告)号：US20110183867A1

公开(公告)日：2011-07-28

申请号：US12672528

申请日：2008-08-07

申请人： Martyn Christopher Davies ,  Morgan Alexander ,  Paul Williams ,  Robert Langer ,  Daniel Anderson ,  Andrew Urquhart

发明人： Martyn Christopher Davies ,  Morgan Alexander ,  Paul Williams ,  Robert Langer ,  Daniel Anderson ,  Andrew Urquhart

IPC分类号： C40B30/00 ,  C40B30/06

CPC分类号： C40B50/14 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00691 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00736 ,  B01J2219/00743 ,  B82Y30/00 ,  C12Q1/04

摘要： This invention relates to a method of screening arrays of polymers having pre-determined surface energies. The polymer arrays of the present invention can be used to screen for microorganism adherence. More specifically the arrays can be used to screen for adherence of particular bacteria or fungi to particular polymers in the array. Furthermore, this invention relates to a method combining in-situ polymer synthesis with physico-chemical characterisation of the resulting polymer array and subsequent biological assays of bacterial or fungal adherence. This allows for high throughput screening and characterisation of candidate polymers which are not susceptible to bacterial or fungal adherence or which can be used to support bacterial or fungal adherence where such is required. The arrays can also be used to screen for inhibition or promotion of biofilm formation.

摘要翻译： 本发明涉及一种筛选具有预定表面能的聚合物阵列的方法。 本发明的聚合物阵列可用于筛选微生物附着。 更具体地，阵列可用于筛选特定细菌或真菌对阵列中特定聚合物的粘附。 此外，本发明涉及将原位聚合物合成与所得聚合物阵列的物理化学表征和随后的细菌或真菌粘附的生物测定相结合的方法。 这允许对细菌或真菌粘附不敏感的候选聚合物进行高通量筛选和表征，或者可以在需要这些聚合物的情况下用于支持细菌或真菌附着。 阵列也可用于筛选抑制或促进生物膜形成。