公开(公告)号：US20130295647A1

公开(公告)日：2013-11-07

申请号：US13881795

申请日：2011-10-28

申请人： Krystyna Dabrowska ,  Anna Oslizlo ,  Paulina Budynek ,  Agnieszka Otrowicz ,  Andrzej Gorski ,  Grzegorz Figura ,  Barbara Owczarek ,  Agnieszka Kopciuch

发明人： Krystyna Dabrowska ,  Anna Oslizlo ,  Paulina Budynek ,  Agnieszka Otrowicz ,  Andrzej Gorski ,  Grzegorz Figura ,  Barbara Owczarek ,  Agnieszka Kopciuch

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/21 ,  C07K2319/23 ,  C12N2795/00051 ,  C12N2795/10122 ,  C12N2795/10151

摘要： The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes.

摘要翻译： 所提出的方法促进用于治疗用途的噬菌体制剂的单阶段并且同时有效净化，并且在噬菌体从树脂置换及其蛋白水解释放的情况下促进噬菌体抗菌活性的维持。 使用合适的结合基序对噬菌体衣壳进行蛋白质修饰使得可以使用亲和层析纯化治疗性噬菌体菌株。 所提出的方法可用于在噬菌体衣壳上显示选择的多肽，而不需要遗传修饰噬菌体，因此使得可以使用天然存在的野生型噬菌体或其它未另外修饰用于噬菌体的其他种类的噬菌体制备各种用途的噬菌体制剂 - 显示目的。

公开(公告)号：US10160956B2

公开(公告)日：2018-12-25

申请号：US13881795

申请日：2011-10-28

申请人： Krystyna Dabrowska ,  Anna Oslizlo ,  Paulina Budynek ,  Agnieszka Otrowicz ,  Andrzej Gorski ,  Grzegorz Figura ,  Barbara Owczarek ,  Agnieszka Kopciuch

发明人： Krystyna Dabrowska ,  Anna Oslizlo ,  Paulina Budynek ,  Agnieszka Otrowicz ,  Andrzej Gorski ,  Grzegorz Figura ,  Barbara Owczarek ,  Agnieszka Kopciuch

IPC分类号： C12N7/00 ,  C07K14/005

摘要： The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes.