公开(公告)号：US09795642B2

公开(公告)日：2017-10-24

申请号：US14779159

申请日：2014-03-25

申请人： TEMPLE UNIVERSITY—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION ,  J. CRAIG VENTER INSTITUTE, INC.

发明人： Roy H. Stevens ,  Hongming Zhang ,  Derrick E. Fouts ,  Jessica DePew

IPC分类号： A61K35/76 ,  A01N63/00 ,  C12N7/00 ,  A61K9/00 ,  A61L26/00

CPC分类号： A61K35/76 ,  A01N63/00 ,  A61K9/0014 ,  A61L26/0057 ,  A61L26/0066 ,  A61L2300/404 ,  C12N7/00 ,  C12N2795/00021 ,  C12N2795/00032 ,  C12N2795/10121 ,  C12N2795/10122 ,  C12N2795/10321 ,  C12N2795/10322

摘要： Bacteriophages are provided that infect strains of Enterococcus faecalis, an opportunistic bacterial pathogen that causes human disease. Also provided are methods of treating Enterococcus faecalis by therapeutic administration of such bacteriophages.

公开(公告)号：US09611470B2

公开(公告)日：2017-04-04

申请号：US14336828

申请日：2014-07-21

申请人： Wisconsin Alumni Research Foundation

发明人： Michael Matthew Cox ,  Angela Jo Gruber ,  Tayla Maria Olsen

IPC分类号： C12N15/01 ,  C12N9/22 ,  C07K14/005

CPC分类号： C12N9/22 ,  C07K14/005 ,  C12N15/01 ,  C12N15/113 ,  C12N2310/00 ,  C12N2795/10122 ,  C12Y301/11

摘要： Purified Ref polypeptides with increased nuclease site-specific targeting activity, recombinant nucleic acids and cells for expression of such Ref polypeptides, and methods for using the Ref polypeptides in combination with RecA protein and variants thereof to effect targeted nuclease cleavage of a DNA duplex are disclosed.

公开(公告)号：US09605250B2

公开(公告)日：2017-03-28

申请号：US14111531

申请日：2012-04-12

申请人： Chemira B. Appaiah ,  Sriram Padmanabhan ,  R. Sanjeev Saravanan ,  Bharathi Sriram

发明人： Chemira B. Appaiah ,  Sriram Padmanabhan ,  R. Sanjeev Saravanan ,  Bharathi Sriram

IPC分类号： A61K38/43 ,  C12N9/24 ,  C12N9/36 ,  C12N1/06 ,  C12N15/62 ,  C07K14/47 ,  C12N9/48 ,  C07K14/005 ,  A61K38/00

CPC分类号： C12N9/2462 ,  A61K38/00 ,  C07K14/005 ,  C07K14/47 ,  C07K14/4742 ,  C07K2319/03 ,  C12N1/06 ,  C12N9/24 ,  C12N9/48 ,  C12N15/62 ,  C12N2795/00022 ,  C12N2795/00033 ,  C12N2795/10122 ,  C12N2795/10133 ,  C12N2795/10322 ,  C12N2795/10333 ,  C12Y302/01017 ,  Y02A50/475 ,  Y02A50/483

摘要： Provided herein are antibacterial compositions and methods of making and using the compositions.

公开(公告)号：US09523101B2

公开(公告)日：2016-12-20

申请号：US14322097

申请日：2014-07-02

申请人： The Catholic University of America

发明人： Venigalla B. Rao

IPC分类号： C12N15/86 ,  C12N15/87 ,  C07K14/005 ,  C12N7/00 ,  C12N15/88 ,  A61K47/48 ,  A61K31/7088 ,  A61K31/711 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

公开(公告)号：US20160264941A1

公开(公告)日：2016-09-15

申请号：US15031763

申请日：2014-10-24

申请人： PHERECYDES PHARMA

发明人： Flavie POUILLOT ,  Hélène BLOIS

IPC分类号： C12N7/00 ,  A61K35/76 ,  C12Q1/18 ,  C07K14/005

CPC分类号： C12N7/00 ,  A61K35/76 ,  C07K14/005 ,  C12N2795/10121 ,  C12N2795/10122 ,  C12N2795/10132 ,  C12N2795/10171 ,  C12N2795/10221 ,  C12N2795/10222 ,  C12N2795/10232 ,  C12N2795/10271 ,  C12Q1/18

摘要： The present invention relates to bacteriophage therapy. More particularly, the present invention relates to novel bacteriophages having a high specificity against Pseudomonas aeruginosa strains, their manufacture, components thereof, compositions comprising the same and the uses thereof in phage therapy.

摘要翻译： 本发明涉及噬菌体治疗。 更具体地，本发明涉及对绿脓假单胞菌菌株具有高特异性的新型噬菌体，其制造方法，其组分，包含其的组合物及其在噬菌体治疗中的用途。

公开(公告)号：US20140335158A1

公开(公告)日：2014-11-13

申请号：US14322097

申请日：2014-07-02

申请人： The Catholic University of America

发明人： Venigalla B. Rao

IPC分类号： C12N15/86 ,  A61K47/48 ,  C12N7/00

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

摘要翻译： 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

公开(公告)号：US20130295647A1

公开(公告)日：2013-11-07

申请号：US13881795

申请日：2011-10-28

申请人： Krystyna Dabrowska ,  Anna Oslizlo ,  Paulina Budynek ,  Agnieszka Otrowicz ,  Andrzej Gorski ,  Grzegorz Figura ,  Barbara Owczarek ,  Agnieszka Kopciuch

发明人： Krystyna Dabrowska ,  Anna Oslizlo ,  Paulina Budynek ,  Agnieszka Otrowicz ,  Andrzej Gorski ,  Grzegorz Figura ,  Barbara Owczarek ,  Agnieszka Kopciuch

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/21 ,  C07K2319/23 ,  C12N2795/00051 ,  C12N2795/10122 ,  C12N2795/10151

摘要： The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes.

摘要翻译： 所提出的方法促进用于治疗用途的噬菌体制剂的单阶段并且同时有效净化，并且在噬菌体从树脂置换及其蛋白水解释放的情况下促进噬菌体抗菌活性的维持。 使用合适的结合基序对噬菌体衣壳进行蛋白质修饰使得可以使用亲和层析纯化治疗性噬菌体菌株。 所提出的方法可用于在噬菌体衣壳上显示选择的多肽，而不需要遗传修饰噬菌体，因此使得可以使用天然存在的野生型噬菌体或其它未另外修饰用于噬菌体的其他种类的噬菌体制备各种用途的噬菌体制剂 - 显示目的。

公开(公告)号：US20100209555A1

公开(公告)日：2010-08-19

申请号：US12665855

申请日：2008-07-02

申请人： Jakob Brandt Borup Haaber ,  Sylvain Moineau ,  Karin Hammer

发明人： Jakob Brandt Borup Haaber ,  Sylvain Moineau ,  Karin Hammer

IPC分类号： C12N1/21 ,  A23C9/12

CPC分类号： A23C9/1234 ,  C07K14/005 ,  C12N2795/10122

摘要： The present invention relates to the field of dairy science. In particular, the present invention relates to methods for improving dairy starter culture quality as well as food products that can be obtained using such methods.

摘要翻译： 本发明涉及乳制品科学领域。 特别地，本发明涉及提高乳制品发酵剂培养质量的方法以及可以使用这些方法获得的食品。

公开(公告)号：US20070014814A1

公开(公告)日：2007-01-18

申请号：US11445455

申请日：2006-06-02

申请人： David Hone ,  David Onyabe

发明人： David Hone ,  David Onyabe

IPC分类号： A61K39/21 ,  C07K14/16

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/53 ,  A61K2039/55544 ,  C07K14/43522 ,  C07K2319/00 ,  C07K2319/735 ,  C12N2730/10122 ,  C12N2740/16122 ,  C12N2760/14122 ,  C12N2795/10122 ,  Y02A50/472

摘要： HIV envelope immunogens that display multivalent epitopes are provided. The immunogens are aggregated, “webbed” HIV envelope immunogens in which native envelope structures are stabilized due to interactions with multimeric derivatives of M9 scorpion toxin.

摘要翻译： 提供显示多价表位的HIV信封免疫原。 免疫原被聚集，“网状”HIV包膜免疫原，其中天然包膜结构由于与M9蝎毒素的多聚衍生物的相互作用而稳定。

公开(公告)号：US20040002058A1

公开(公告)日：2004-01-01

申请号：US10329987

申请日：2002-12-26

申请人： UAB Research Foundation

发明人： Larry Cosenza

IPC分类号： C12Q001/70 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  C07K014/005

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K2299/00 ,  C07K2319/00 ,  C12N2770/32322 ,  C12N2795/10122

摘要： The present invention encompasses chimeric capsid proteins, nucleic acids encoding such proteins and capsids containing chimeric capsid proteins. Methods of making the chimeric capsid proteins, the nucleic acids that encode such proteins and capsids that contain chimeric capsid proteins are also encompassed within the scope of the invention. The invention further encompasses the use of the chimeric capsid proteins to produce protein elements and to present the elements for use in structure-function studies, for use as therapeutic factors and for other purposes. Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only.

摘要翻译： 本发明包括嵌合衣壳蛋白，编码这种蛋白质的核酸和含有嵌合衣壳蛋白的衣壳。 制备嵌合衣壳蛋白，编码这种蛋白质的核酸和含有嵌合衣壳蛋白的衣壳的方法也包括在本发明的范围内。 本发明还包括嵌合衣壳蛋白用于产生蛋白质元件并呈现用于结构功能研究的元件，用作治疗因素和用于其它目的的用途。 通过考虑本文公开的本发明的说明书和实践，本发明的其它实施例对于本领域技术人员将是显而易见的。 旨在将说明书和实施例视为仅作为示例。