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    Purified bacteriophage, its preparation and application
    3.
    发明授权
    Purified bacteriophage, its preparation and application 有权
    纯化噬菌体，其制备及应用

    公开(公告)号：US09255251B2

    公开(公告)日：2016-02-09

    申请号：US12449418

    申请日：2008-02-08

    申请人： Tomasz Lipinski ,  Andrzej Gamian ,  Ewa Zuziak ,  Agnieszka Korzeniowska-Kowal ,  Andrzej Gorski

    发明人： Tomasz Lipinski ,  Andrzej Gamian ,  Ewa Zuziak ,  Agnieszka Korzeniowska-Kowal ,  Andrzej Gorski

    IPC分类号： C12N7/00 ,  C12N7/02 ,  A61K35/76

    CPC分类号： C12N7/00 ,  A61K35/76 ,  C12N7/02 ,  C12N2795/00011 ,  C12N2795/00051 ,  C12N2795/10132 ,  C12N2795/10151

    摘要： A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.

    摘要翻译： 制备具有增加的抗菌活性的纯化噬菌体的方法，其中从噬菌体的细菌裂解物中获得有利的是在溶菌酶，螯合因子和洗涤剂的存在下，以连续的方式在膜上进行超滤，所述噬菌体含有高分子量制备 ，没有细菌细胞壁和其他污染物，没有毒素和内毒素，在细菌裂解试验中有活性，其特征为色谱HPLC，SDS-PAGE电泳，免疫印迹，细菌裂解生物试验，专用于噬菌体治疗 的细菌感染和肿瘤以及用于生产噬菌体衍生的药物制剂。

    METHOD OF PRODUCING A PHARMACOLOGICALLY STABLE FORM OF LYOPHILISED, ACTIVE PREPARATIONS OF PURIFIED BACTERIOPHAGES
    6.
    发明申请
    METHOD OF PRODUCING A PHARMACOLOGICALLY STABLE FORM OF LYOPHILISED, ACTIVE PREPARATIONS OF PURIFIED BACTERIOPHAGES 有权
    生产LYOPHILIS，活性制剂的纯化菌株的药理学稳定形式的方法

    公开(公告)号：US20110256103A1

    公开(公告)日：2011-10-20

    申请号：US13059815

    申请日：2009-10-29

    申请人： Ewa Zuziak ,  Andrzej Gamian ,  Andrzej Gorski ,  Tomasz Lipinski

    发明人： Ewa Zuziak ,  Andrzej Gamian ,  Andrzej Gorski ,  Tomasz Lipinski

    IPC分类号： A61K35/76 ,  A61P31/04 ,  A61P35/00

    CPC分类号： C12N7/00 ,  A61K35/13 ,  C12N2795/10051 ,  C12N2795/10151

    摘要： The subject the present invention is a method of producing a pharmacologically stable form of purified and lyophilised bacteriophage preparations of increased stability and antibacterial activity, characterised in that phages produced from a bacterial lysate, for example by ultrafiltration on ultrafiltration membranes, containing a high molecular weight preparation of purified phages is stabilised most preferably with a probiotic extract in the presence or absence of neutral salts and/or organic solvents, lyophilised, characterised via HPLC chromatography, by SDS-PAGE, and bacterial lysis biological assays, and then stored under a vacuum, where the active lyophilisate is destined for phage therapy of infections and tumours.

    摘要翻译： 本发明的主题是制备具有增加的稳定性和抗菌活性的纯化和冻干的噬菌体制剂的药理稳定形式的方法，其特征在于从细菌裂解物产生的噬菌体，例如通过在超滤膜上超滤，含有高分子量 纯化噬菌体的制备在存在或不存在中性盐和/或有机溶剂的情况下最优选用益生菌提取物稳定，冻干，通过HPLC色谱法，通过SDS-PAGE表征，细菌裂解生物测定法，然后在真空下储存 ，其中活性冻干物用于感染和肿瘤的噬菌体治疗。

    PURIFIED BACTERIOPHAGE, ITS PREPARATION AND APPLICATION
    7.
    发明申请
    PURIFIED BACTERIOPHAGE, ITS PREPARATION AND APPLICATION 有权
    纯化细菌，其制备和应用

    公开(公告)号：US20110008873A1

    公开(公告)日：2011-01-13

    申请号：US12449418

    申请日：2008-02-08

    申请人： Tomasz Lipinski ,  Andrzej Gamian ,  Ewa Zuziak ,  Agnieszka Korzeniowska-Kowal ,  Andrzej Gorski

    发明人： Tomasz Lipinski ,  Andrzej Gamian ,  Ewa Zuziak ,  Agnieszka Korzeniowska-Kowal ,  Andrzej Gorski

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  A61K35/76 ,  C12N7/02 ,  C12N2795/00011 ,  C12N2795/00051 ,  C12N2795/10132 ,  C12N2795/10151

    摘要： A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations.

    摘要翻译： 制备具有增加的抗菌活性的纯化噬菌体的方法，其中从噬菌体的细菌裂解物中获得有利的是在溶菌酶，螯合因子和洗涤剂的存在下，以连续的方式在膜上进行超滤，所述噬菌体含有高分子量制备 ，没有细菌细胞壁和其他污染物，没有毒素和内毒素，在细菌裂解试验中有活性，其特征为色谱HPLC，SDS-PAGE电泳，免疫印迹，细菌裂解生物试验，专用于噬菌体治疗 的细菌感染和肿瘤以及用于生产噬菌体衍生的药物制剂。