公开(公告)号：US20090053790A1

公开(公告)日：2009-02-26

申请号：US12223464

申请日：2007-01-31

申请人： Kuni Fushikida ,  Yoshitaka Kondo

发明人： Kuni Fushikida ,  Yoshitaka Kondo

IPC分类号： C12N7/00 ,  C07K14/00 ,  C12N5/00 ,  C12N1/00 ,  C07H21/00

CPC分类号： C07K14/005 ,  C07K2319/70 ,  C12N2760/18822 ,  C12N2760/18845 ,  C12N2810/6072

摘要： Proteins are provided for transferring a protein, an antibody or another foreign substance into a cell without impairing the function or structure thereof; and methods of transferring a foreign substance into a cell in a time and quantity controllable manner at a high efficiency by using the above-described delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein. As the results of intensive studies on a method of enclosing a foreign substance in the envelope of an envelope virus, it is found out that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, it is found out that use of the aforementioned protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore the resulting foreign substance-containing envelope virus or inactivated envelope virus makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

摘要翻译： 提供蛋白质用于将蛋白质，抗体或其他异物转移到细胞中而不损害其功能或结构; 以及通过使用上述递送蛋白或包膜病毒或灭活的包膜病毒与所述递送蛋白组合，以高效率以时间和数量可控的方式将异物转移到细胞中的方法。 作为将外来物质包封在信封病毒的包膜中的方法的深入研究的结果，发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于外源的有效封闭 物质在信封。 此外，发现使用上述蛋白质可使外来物质被包含在信封病毒或灭活的包膜病毒中，因此产生的含异物的包膜病毒或灭活的包膜病毒能够有效地将物质转移到细胞中 而不损害其生理功能。

公开(公告)号：US08388979B2

公开(公告)日：2013-03-05

申请号：US13546359

申请日：2012-07-11

申请人： Kuni Fushikida ,  Yoshitaka Kondo

发明人： Kuni Fushikida ,  Yoshitaka Kondo

IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/385 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/567 ,  C12P1/00

CPC分类号： C07K14/005 ,  C07K2319/70 ,  C12N2760/18822 ,  C12N2760/18845 ,  C12N2810/6072

摘要： Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

摘要翻译： 提供递送蛋白质用于将蛋白质，抗体或异物转移到细胞中而不损害其功能或结构。 此外，提供了通过使用递送蛋白或包膜病毒或灭活的包膜病毒结合所述递送蛋白将异物高效转移到细胞中的方法。 本发明人发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于将外源物质有效地包封在信封中。 此外，本发明人发现，使用递送蛋白质使得外来物质能够包含在包膜病毒或灭活的包膜病毒中，因此能够将物质有效地转移到细胞中而不损害其生理功能。

公开(公告)号：US20110318792A1

公开(公告)日：2011-12-29

申请号：US12377230

申请日：2007-08-14

申请人： Toshio Ohsuga ,  Kuni Fushikida

发明人： Toshio Ohsuga ,  Kuni Fushikida

IPC分类号： C12P13/12 ,  C12N1/19 ,  C12N1/15

CPC分类号： C12P19/40 ,  C12N9/1288

摘要： The object of the present invention is to provide a microbial mutant capable of producing S-adenosylmethionine (hereinafter referred to as SAM) in a large amount with no change in growth characteristics and a method of efficiently producing SAM by incubating the microbial mutant.The method of producing S-adenosylmethionine comprises incubating a microorganism having a loss or a reduction in the function of an enzyme relating to synthesis of phosphatidylcholine and recovering S-adenosylmethionine accumulated in the culture.

摘要翻译： 本发明的目的是提供一种生长特性没有变化的能够大量产生S-腺苷甲硫氨酸（以下称为SAM）的微生物突变体，以及通过温育微生物突变体有效地生产SAM的方法。 生产S-腺苷甲硫氨酸的方法包括将与磷脂酰胆碱合成有关的酶的功能丧失或降低的微生物孵育并回收在培养物中积累的S-腺苷甲硫氨酸。