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    POLYPEPTIDE HAVING AFFINITY FOR ENVELOPE VIRUS CONSTITUENT AND USE THEREOF IN TRANSFERRING SUBSTANCE INTO CELL
    1.
    发明申请
    POLYPEPTIDE HAVING AFFINITY FOR ENVELOPE VIRUS CONSTITUENT AND USE THEREOF IN TRANSFERRING SUBSTANCE INTO CELL 有权
    具有包容性病毒组织的亲和力的多肽及其在细胞转运中的用途

    公开(公告)号：US20120301961A1

    公开(公告)日：2012-11-29

    申请号：US13546359

    申请日：2012-07-11

    申请人： Kuni FUSHIKIDA ,  Yoshitaka Kondo

    发明人： Kuni FUSHIKIDA ,  Yoshitaka Kondo

    IPC分类号： C12N5/071

    CPC分类号： C07K14/005 ,  C07K2319/70 ,  C12N2760/18822 ,  C12N2760/18845 ,  C12N2810/6072

    摘要： Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

    摘要翻译： 提供递送蛋白质用于将蛋白质，抗体或异物转移到细胞中而不损害其功能或结构。 此外，提供了通过使用递送蛋白或包膜病毒或灭活的包膜病毒结合所述递送蛋白将异物高效转移到细胞中的方法。 本发明人发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于将外源物质有效地包封在信封中。 此外，本发明人发现，使用递送蛋白质使得外来物质能够包含在包膜病毒或灭活的包膜病毒中，因此能够将物质有效地转移到细胞中而不损害其生理功能。

    Polypeptide having affinity for envelope virus constituent and use thereof in transferring substance into cell
    2.
    发明授权
    Polypeptide having affinity for envelope virus constituent and use thereof in transferring substance into cell 有权
    对包膜病毒成分具有亲和性的多肽及其将物质转移至细胞中的用途

    公开(公告)号：US08388979B2

    公开(公告)日：2013-03-05

    申请号：US13546359

    申请日：2012-07-11

    申请人： Kuni Fushikida ,  Yoshitaka Kondo

    发明人： Kuni Fushikida ,  Yoshitaka Kondo

    IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/385 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/567 ,  C12P1/00

    CPC分类号： C07K14/005 ,  C07K2319/70 ,  C12N2760/18822 ,  C12N2760/18845 ,  C12N2810/6072

    摘要： Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

    摘要翻译： 提供递送蛋白质用于将蛋白质，抗体或异物转移到细胞中而不损害其功能或结构。 此外，提供了通过使用递送蛋白或包膜病毒或灭活的包膜病毒结合所述递送蛋白将异物高效转移到细胞中的方法。 本发明人发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于将外源物质有效地包封在信封中。 此外，本发明人发现，使用递送蛋白质使得外来物质能够包含在包膜病毒或灭活的包膜病毒中，因此能够将物质有效地转移到细胞中而不损害其生理功能。

    Mutant paramyxovirus and method for production thereof
    3.
    发明授权
    Mutant paramyxovirus and method for production thereof 有权
    突变型副粘病毒及其生产方法

    公开(公告)号：US07858356B2

    公开(公告)日：2010-12-28

    申请号：US12085355

    申请日：2006-11-24

    申请人： Yasufumi Kaneda ,  Katsuto Tamai ,  Kotaro Saga ,  Masako Kawachi

    发明人： Yasufumi Kaneda ,  Katsuto Tamai ,  Kotaro Saga ,  Masako Kawachi

    IPC分类号： C12N7/01 ,  C12N7/02 ,  C12N5/10 ,  C12N15/86 ,  C12N15/62 ,  C12N15/45 ,  C07K14/115 ,  C07K19/00

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/30 ,  C07K2319/33 ,  C12N15/1131 ,  C12N15/86 ,  C12N2310/14 ,  C12N2760/18821 ,  C12N2760/18822 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2810/859

    摘要： The present invention provides a modified paramyxovirus containing a reduced amount of receptor-binding protein compared with the wild type; a method of preparing a modified paramyxovirus, comprising the following steps: (1) a step for introducing a nucleic acid that suppresses the expression of a receptor-binding protein of a paramyxovirus into an animal cell, (2) a step for infecting the paramyxovirus to the cell, and (3) a step for isolating paramyxovirus particles replicated in the cell; and a modified paramyxovirus prepared by the method of preparation mentioned above.The present invention also provides a chimera protein wherein a fusion protein of a virus has been joined or bound to a peptide that binds specifically to a cell surface marker; a nucleic acid that encodes the chimera protein; an animal cell capable of expressing the chimera protein on the cell surface thereof; a modified paramyxovirus expressing the chimera protein on the virus particle surface thereof; and a method of preparing a tissue targeting paramyxovirus, comprising: (1) a step for supplying a nucleic acid that encodes a chimera protein wherein a fusion protein of a virus has been joined or bound to a peptide that binds specifically to a cell surface marker of the target cells, (2) a step for introducing the nucleic acid supplied in (1) into an animal cell in an expressible state, and expressing the same, (3) a step for infecting a paramyxovirus to the cell, and (4) a step for isolating paramyxovirus particles replicated in the cell.

    摘要翻译： 本发明提供了与野生型相比含有减少量的受体结合蛋白的修饰的副粘病毒; 一种制备修饰的副粘病毒的方法，包括以下步骤：（1）将抑制副粘病毒的受体结合蛋白的表达的核酸引入动物细胞的步骤，（2）感染副粘病毒的步骤 （3）用于分离复制在细胞中的副粘病毒颗粒的步骤; 和通过上述制备方法制备的修饰的副粘病毒。 本发明还提供一种嵌合蛋白，其中病毒的融合蛋白已经结合或结合到特异性结合细胞表面标记的肽; 编码嵌合蛋白的核酸; 能够在其细胞表面上表达嵌合体蛋白质的动物细胞; 在病毒颗粒表面上表达嵌合体蛋白质的修饰的副粘病毒; 以及制备组织靶向副粘病毒的方法，包括：（1）提供编码嵌合体蛋白的核酸的步骤，其中病毒的融合蛋白已经结合或与特异性结合于细胞表面标记的肽结合 的靶细胞，（2）将（1）中提供的核酸以可表达状态引入动物细胞中并表达其的步骤，（3）感染细胞的副粘病毒的步骤，（4 ）分离在细胞中复制的副粘病毒颗粒的步骤。

    Envelope gene-deficient paramyxovirus vector
    6.
    发明申请
    Envelope gene-deficient paramyxovirus vector 有权
    信封基因缺陷型副粘病毒载体

    公开(公告)号：US20030170266A1

    公开(公告)日：2003-09-11

    申请号：US10316535

    申请日：2002-12-10

    发明人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Fumino Tokito ,  Takahiro Hirata ,  Tsuyoshi Tokusumi ,  Makoto Inoue ,  Yumiko Tokusumi

    IPC分类号： C12N007/00 ,  C12N015/86 ,  C12P021/06 ,  A61K039/12 ,  A61K039/155 ,  C12N007/01 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2760/18823 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2760/18861 ,  C12N2800/30 ,  C12N2810/6081

    摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.

    摘要翻译： 通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收F基因缺陷型病毒粒子。 此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。 此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。 此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。 通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。 此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。 从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。 此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。 用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。

    SOMATIC CELL NUCLEAR TRANSFER METHODS
    7.
    发明申请
    SOMATIC CELL NUCLEAR TRANSFER METHODS 有权
    SOMATIC CELL NUCLEAR TRANSFER方法

    公开(公告)号：US20160024528A1

    公开(公告)日：2016-01-28

    申请号：US14775594

    申请日：2014-03-14

    申请人： Dietrich M. Egli

    发明人： Dietrich M. Egli

    IPC分类号： C12N15/877

    CPC分类号： C12N15/8776 ,  C12N5/0606 ,  C12N5/0609 ,  C12N2501/065 ,  C12N2501/31 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2517/04 ,  C12N2517/10 ,  C12N2760/18845

    摘要： The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods.

    摘要翻译： 本发明提供了制备包含人体细胞的二倍体基因组的重建的二倍体人卵母细胞的方法，以及用于制备人核转移胚胎，人胚胎干细胞和人分化细胞的方法。 本发明还提供重建的人类卵母细胞，人类核转移胚胎，人类胚胎干细胞以及使用这些方法制备的分化细胞，以及可用于进行这些方法的组合物和试剂盒。

    Polypeptide Having Affinity for Envelope Virus Constituent and Use Thereof in Transferring Substance Into Cell
    8.
    发明申请
    Polypeptide Having Affinity for Envelope Virus Constituent and Use Thereof in Transferring Substance Into Cell 审中-公开
    具有包膜病毒成分亲和力的多肽和将其转移到细胞中的用途

    公开(公告)号：US20090053790A1

    公开(公告)日：2009-02-26

    申请号：US12223464

    申请日：2007-01-31

    申请人： Kuni Fushikida ,  Yoshitaka Kondo

    发明人： Kuni Fushikida ,  Yoshitaka Kondo

    IPC分类号： C12N7/00 ,  C07K14/00 ,  C12N5/00 ,  C12N1/00 ,  C07H21/00

    CPC分类号： C07K14/005 ,  C07K2319/70 ,  C12N2760/18822 ,  C12N2760/18845 ,  C12N2810/6072

    摘要： Proteins are provided for transferring a protein, an antibody or another foreign substance into a cell without impairing the function or structure thereof; and methods of transferring a foreign substance into a cell in a time and quantity controllable manner at a high efficiency by using the above-described delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein. As the results of intensive studies on a method of enclosing a foreign substance in the envelope of an envelope virus, it is found out that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, it is found out that use of the aforementioned protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore the resulting foreign substance-containing envelope virus or inactivated envelope virus makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

    摘要翻译： 提供蛋白质用于将蛋白质，抗体或其他异物转移到细胞中而不损害其功能或结构; 以及通过使用上述递送蛋白或包膜病毒或灭活的包膜病毒与所述递送蛋白组合，以高效率以时间和数量可控的方式将异物转移到细胞中的方法。 作为将外来物质包封在信封病毒的包膜中的方法的深入研究的结果，发现含有对包膜病毒成分具有亲和性的多肽的蛋白质有助于外源的有效封闭 物质在信封。 此外，发现使用上述蛋白质可使外来物质被包含在信封病毒或灭活的包膜病毒中，因此产生的含异物的包膜病毒或灭活的包膜病毒能够有效地将物质转移到细胞中 而不损害其生理功能。

    Envelope gene-deficient Paramyxovirus vector
    9.
    发明授权
    Envelope gene-deficient Paramyxovirus vector 有权
    包膜基因缺陷型副粘病毒载体

    公开(公告)号：US07226786B2

    公开(公告)日：2007-06-05

    申请号：US10316535

    申请日：2002-12-10

    申请人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Fumino Tokito ,  Takahiro Hirata ,  Tsuyoshi Tokusumi ,  Makoto Inoue ,  Yumiko Tokusumi

    发明人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Fumino Tokito ,  Takahiro Hirata ,  Tsuyoshi Tokusumi ,  Makoto Inoue ,  Yumiko Tokusumi

    IPC分类号： C12N15/00 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2760/18823 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2760/18861 ,  C12N2800/30 ,  C12N2810/6081

    摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.

    摘要翻译： 通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收了F基因缺陷型病毒粒子。 此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。 此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。 此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。 通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。 此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。 从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。 此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。 用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。