公开(公告)号：US08173558B2

公开(公告)日：2012-05-08

申请号：US11628759

申请日：2005-06-07

申请人： Kunihiro Fukuoka ,  Koji Nishio ,  Seiji Yamahara ,  Takahiro Yamazaki ,  Takashi Maruoka ,  Fumiyuki Yamasaki ,  Susumu Kibune ,  Tsutomu Suzuoki ,  Shigeo Souda ,  Taisuke Yamamoto ,  Kouji Kimura ,  Shinobu Tabata

发明人： Kunihiro Fukuoka ,  Koji Nishio ,  Seiji Yamahara ,  Takahiro Yamazaki ,  Takashi Maruoka ,  Fumiyuki Yamasaki ,  Susumu Kibune ,  Tsutomu Suzuoki ,  Shigeo Souda ,  Taisuke Yamamoto ,  Kouji Kimura ,  Shinobu Tabata

IPC分类号： D04B1/14 ,  D04B1/18 ,  D04B1/22 ,  D04B9/34 ,  B29C47/88

CPC分类号： D04B1/18 ,  D10B2331/10 ,  D10B2403/0114 ,  Y10T442/413 ,  Y10T442/438 ,  Y10T442/45

摘要： A polyurethane elastomeric filament-containing weft knit fabric is obtained by plating a bare yarn of highly fusible, alkali-resistant polyurethane elastomeric filament having at least 50% retention of tenacity following dry heat treatment under 100% extension at 150° C. for 45 seconds, a melting point of 180° C. or below, and at least 60% retention of tenacity following treatment in a 2 g/L aqueous sodium hydroxide solution under 100% extension at 100° C. for 60 minutes at every loop of a weft knit fabric having a 1×1 rib knit structure or a center yarn-containing reversible knit structure composed of at least one type of non-elastomeric yarn, then heat setting the plated structure so as to thermally fuse the highly fusible, alkali-resistant polyurethane elastomeric filaments to each other or to the non-elastomeric yarns at crossover points therebetween.

摘要翻译： 聚氨酯弹性体长丝的纬编针织物通过在150℃延伸100％延伸干燥热处理后镀覆高熔点，耐碱性聚氨酯弹性体丝的裸线，其具有至少50％的强度保持力，持续45秒 ，熔点为180℃或更低，并且在100g延伸100％的2g / L氢氧化钠水溶液中处理后，在纬纱的每个环圈处理60分钟后处理至少60％的强度 具有1×1罗纹针织结构的针织物或由至少一种类型的非弹性纱线构成的中心纱线可逆编织结构，然后热定形镀层结构，以将高熔点，耐碱聚氨酯 弹性纤维彼此之间或其间的交叉点处的非弹性纱线。

公开(公告)号：US20080032580A1

公开(公告)日：2008-02-07

申请号：US11628759

申请日：2005-06-07

申请人： Kunihiro Fukuoka ,  Koji Nishio ,  Seiji Yamahara ,  Takahiro Yamazaki ,  Takashi Maruoka ,  Fumiyuki Yamasaki ,  Susumu Kibune ,  Tsutomu Suzuoki ,  Shigeo Souda ,  Taisuke Yamamoto ,  Kouji Kimura ,  Shinobu Tabata

发明人： Kunihiro Fukuoka ,  Koji Nishio ,  Seiji Yamahara ,  Takahiro Yamazaki ,  Takashi Maruoka ,  Fumiyuki Yamasaki ,  Susumu Kibune ,  Tsutomu Suzuoki ,  Shigeo Souda ,  Taisuke Yamamoto ,  Kouji Kimura ,  Shinobu Tabata

IPC分类号： D04B1/18

CPC分类号： D04B1/18 ,  D10B2331/10 ,  D10B2403/0114 ,  Y10T442/413 ,  Y10T442/438 ,  Y10T442/45

摘要： A polyurethane elastomeric filament-containing weft knit fabric is obtained by plating a bare yarn of highly fusible, alkali-resistant polyurethane elastomeric filament having at least 50% retention of tenacity following dry heat treatment under 100% extension at 150° C. for 45 seconds, a melting point of 180° C. or below, and at least 60% retention of tenacity following treatment in a 2 g/L aqueous sodium hydroxide solution under 100% extension at 100° C. for 60 minutes at every loop of a weft knit fabric having a 1×1 rib. knit structure or a center yarn-containing reversible knit structure composed of at least one type of non-elastomeric yarn, then heat setting the plated structure so as to thermally fuse the highly fusible, alkali-resistant polyurethane elastomeric filaments to each other or to the non-elastomeric yarns at crossover points therebetween.

摘要翻译： 聚氨酯弹性体长丝的纬编针织物通过在150℃延伸100％延伸干燥热处理后镀覆高熔点，耐碱性聚氨酯弹性体丝的裸线，其具有至少50％的强度保持力，持续45秒 ，熔点为180℃或更低，并且在100g延伸100％的2g / L氢氧化钠水溶液中处理后，在纬纱的每个环圈处理60分钟后处理至少60％的强度 具有1x1肋的针织物。 针织结构或由至少一种类型的非弹性纱线组成的中心纱线可逆编织结构，然后热定形镀层结构，以将高度易熔，耐碱的聚氨酯弹性体丝相互熔合或彼此 非弹性纱线在其间的交叉点处。

公开(公告)号：US20090297490A1

公开(公告)日：2009-12-03

申请号：US12282977

申请日：2007-03-15

申请人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

发明人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

IPC分类号： A61K35/12 ,  C12N5/08

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K2035/122 ,  A61K2300/00

摘要： The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features: (1) being positive for CD3, CD25, CD28 and T-cell antigen receptor αβ; (2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8−) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4−CD8+) T-cell group; (3) exerting a cytotoxic activity against the cocultured stroma cells; and (4) exerting an immunosuppressive activity against activated T cells.

摘要翻译： 本发明的目的是提供具有细胞毒性和免疫抑制活性两者的新型人T细胞群及其制备方法。 上述目的是通过提供通过共培养从人血液收集的单核细胞与基质细胞获得的人类T细胞群而获得的，并且具有以下特征：（1）对于CD3，CD25，CD28和T- 细胞抗原受体 （2）基本上由CD4阳性和CD8阳性（CD4 + CD8-）T细胞组，CD4阳性和CD8阳性（CD4 + CD8dim）T细胞组和CD4阴性和CD8阳性三组组成 （CD4-CD8 +）T细胞组; （3）对共培养的基质细胞发挥细胞毒活性; 和（4）对活化的T细胞施加免疫抑制活性。

公开(公告)号：US08883495B2

公开(公告)日：2014-11-11

申请号：US12282977

申请日：2007-03-15

申请人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

发明人： Shuji Nakamura ,  Ryuichi Motoda ,  Masayoshi Kibata ,  Kunzo Orita ,  Fumiyuki Yamasaki

IPC分类号： C12N5/00 ,  A61K35/14 ,  C12N5/0783 ,  A61K35/12

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K2035/122 ,  A61K2300/00

摘要： The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features: (1) being positive for CD3, CD25, CD28 and T-cell antigen receptor αβ; (2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8+) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4−CD8+) T-cell group; (3) exerting a cytotoxic activity against the cocultured stroma cells; and (4) exerting an immunosuppressive activity against activated T cells.

摘要翻译： 本发明的目的是提供具有细胞毒性和免疫抑制活性两者的新型人T细胞群及其制备方法。 上述目的是通过提供通过共培养从人血液收集的单核细胞与基质细胞获得的人类T细胞群而获得的，并且具有以下特征：（1）对于CD3，CD25，CD28和T- 细胞抗原受体α＆bgr; （2）基本上由CD4阳性和CD8阳性（CD4 + CD8 +）T细胞组，CD4阳性和CD8阳性（CD4 + CD8dim）T细胞组和CD4阴性和CD8阳性（CD4 + CD4-CD8 +）T细胞组; （3）对共培养的基质细胞发挥细胞毒活性; 和（4）对活化的T细胞施加免疫抑制活性。

公开(公告)号：US08492146B2

公开(公告)日：2013-07-23

申请号：US13265942

申请日：2010-04-15

申请人： Takeshi Otani ,  Makoto Takeuchi ,  Shuji Nakamura ,  Fumiyuki Yamasaki

发明人： Takeshi Otani ,  Makoto Takeuchi ,  Shuji Nakamura ,  Fumiyuki Yamasaki

IPC分类号： C12N5/0783

CPC分类号： C12N5/0638 ,  A61K2035/122 ,  A61K2035/124 ,  A61K2039/5158 ,  A61K2039/57 ,  C12N5/0637 ,  C12N2502/1394

摘要： A human T cell population which has both cytotoxic and immunosuppressive activities, is efficiently produced by first fractionating CD2-positive CD14-negative cells from mononuclear cells collected from a human umbilical cord blood, and then co-culturing them with stromal cells. The resulting blast cells, which have the desired activity, are proliferated by further culture.

摘要翻译： 通过首先从收集自人脐带血的单核细胞中分离CD2阳性CD14阴性细胞，然后与基质细胞共培养，有效地产生具有细胞毒性和免疫抑制活性的人类T细胞群体。 通过进一步培养来增殖具有所需活性的所得胚细胞。

公开(公告)号：US20120040454A1

公开(公告)日：2012-02-16

申请号：US13265942

申请日：2010-04-15

申请人： Takeshi Otani ,  Makoto Takeuchi ,  Shuji Nakamura ,  Fumiyuki Yamasaki

发明人： Takeshi Otani ,  Makoto Takeuchi ,  Shuji Nakamura ,  Fumiyuki Yamasaki

IPC分类号： C12N5/0783

CPC分类号： C12N5/0638 ,  A61K2035/122 ,  A61K2035/124 ,  A61K2039/5158 ,  A61K2039/57 ,  C12N5/0637 ,  C12N2502/1394

摘要： The present invention has an object to provide a method for efficiently producing a human T cell population which has both cytotoxic and immunosuppressive activities, and solves the above object by providing a method for producing a human T cell population which has both cytotoxic and immunosuppressive activities, comprising the following steps (1) to (4):(1) fractionating mononuclear cells collected from a human umbilical cord blood into CD14-positive (CD14+) cells and CD14-negative (CD14−) cells, and then fractionating the CD14-negative (CD14−) cells into CD2-positive CD14-negative (CD2+CD14−) cells, and CD2-negative CD14-negative (CD2−CD14−) cells;(2) co-culturing the CD2-positive CD14-negative (CD2+CD14−) cells obtained in step (1) with stromal cells to generate blast cells;(3) adding the blast cells obtained in step (2) to the co-culture of the CD14-positive (CD14+) cells obtained in step (1) with stromal cells to allow the blast cells to proliferate; and(4) allowing the blast cells obtained in step (3) to further proliferate by co-culturing with stromal cells in the presence of interleukin-2 (IL-2) to generate a human T cell population which has both cytotoxic and immunosuppressive activities.

摘要翻译： 本发明的目的是提供一种有效地制备具有细胞毒性和免疫抑制活性的人T细胞群的方法，通过提供具有细胞毒性和免疫抑制活性的人T细胞群的制备方法来解决上述目的， 包括以下步骤（1）至（4）：（1）将从人脐带血中收集的单核细胞分离成CD14阳性（CD14 +）细胞和CD14阴性（CD14-）细胞，然后将CD14阴性 （CD14-）细胞进入CD2阳性CD14阴性（CD2 + CD14-）细胞和CD2阴性CD14阴性（CD2-CD14-）细胞; （2）在步骤（1）中获得的CD2阳性CD14-阴性（CD2 + CD14-）细胞与基质细胞共培养以产生胚细胞; （3）将步骤（2）中获得的胚细胞添加到步骤（1）中获得的CD14阳性（CD14 +）细胞与基质细胞的共培养物中，以使胚细胞增殖; 和（4）通过在白细胞介素-2（IL-2）存在下与基质细胞共培养使得步骤（3）中获得的胚细胞进一步增殖以产生具有细胞毒性和免疫抑制活性的人T细胞群 。