公开(公告)号：US09470612B2

公开(公告)日：2016-10-18

申请号：US14122373

申请日：2012-06-26

申请人： Raj Rajagopal ,  Kurt J. Halverson ,  Ramasubramani Kuduva Raman Thanumoorthy

发明人： Raj Rajagopal ,  Kurt J. Halverson ,  Ramasubramani Kuduva Raman Thanumoorthy

IPC分类号： G01N1/40 ,  B01L3/00 ,  G01N15/04 ,  C12Q1/04

CPC分类号： G01N1/4077 ,  B01L3/5021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2400/0409 ,  C12Q1/04 ,  G01N15/042 ,  G01N2001/4083 ,  G01N2001/4088

摘要： Systems and methods for detecting an analyte of interest in a sample. The system can include a first container comprising a filter portion. The filter portion can include a filter comprising a filtrand of the sample on a first side of the filter. The system can further include a second container comprising the filter portion coupled to a detection portion comprising a microstructured surface. The method can include providing the first container, coupling the filter portion to the detection portion to form the second container where the first side of the filter faces the microstructured surface, and centrifuging the second container toward the microstructured surface. The method can further include inverting the second container after centrifuging to decant the supernatant from the detection portion, such that a concentrate comprising a sediment of the sample is retained in the microstructured surface, which can be interrogated for an analyte of interest.

摘要翻译： 用于检测样品中感兴趣的分析物的系统和方法。 该系统可以包括包括过滤器部分的第一容器。 过滤器部分可以包括在过滤器的第一侧上包括样品的过滤器的过滤器。 该系统还可以包括第二容器，其包括耦合到包括微结构化表面的检测部分的过滤器部分。 该方法可以包括提供第一容器，将过滤部分连接到检测部分，以形成第二容器，其中过滤器的第一侧面向微结构化表面，并将第二容器离心到微结构化表面。 该方法还可包括在离心后将第二容器倒置以从检测部分倾析上清液，使得包含样品沉淀物的浓缩物保留在微结构化表面中，该微结构化表面可被询问用于感兴趣的分析物。

公开(公告)号：US09488563B2

公开(公告)日：2016-11-08

申请号：US14122343

申请日：2012-06-26

申请人： Kurt J. Halverson ,  Raj Rajagopal ,  Ramasubramani Kuduva Raman Thanumoorthy

发明人： Kurt J. Halverson ,  Raj Rajagopal ,  Ramasubramani Kuduva Raman Thanumoorthy

IPC分类号： G01N15/04 ,  B01L3/00 ,  G01N1/40

CPC分类号： G01N15/042 ,  B01L3/5021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12Q1/02 ,  G01N1/4077 ,  G01N15/04 ,  G01N21/07 ,  G01N2001/4083 ,  G01N2015/0065 ,  G01N2015/045 ,  G01N2333/245 ,  Y10T436/25375 ,  Y10T436/255

摘要： Method for detecting an analyte of interest in a sample. The method can include providing a container comprising a microstructured surface, and centrifuging the container toward the microstructured surface to form a sediment and a supernatant of the sample. Following centrifugation, the container can be inverted to decant at least a portion of the supernatant of the sample from the second portion, such that a concentrate (e.g., comprising the sediment) of the sample is retained in the microstructured surface. The concentrate can then be interrogated in the microstructured surface for the analyte of interest. In some embodiments, at least a portion of the second portion can be substantially transparent, such that the concentrate can be interrogated from the outside of the container, without requiring that the container be opened prior to interrogation.

摘要翻译： 用于检测样品中感兴趣的分析物的方法。 该方法可以包括提供包含微结构化表面的容器，并将容器离心到微结构化表面以形成样品的沉淀物和上清液。 离心后，将容器倒置以从第二部分倾析样品的上清液的至少一部分，使得样品的浓缩物（例如包含沉淀物）保留在微结构化表面中。 然后可以在微结构化表面中询问浓缩物用于感兴趣的分析物。 在一些实施例中，第二部分的至少一部分可以是基本上透明的，从而可以从容器的外部询问浓缩物，而不需要在询问之前打开容器。

公开(公告)号：US20140096598A1

公开(公告)日：2014-04-10

申请号：US14122343

申请日：2012-06-26

申请人： Kurt J. Halverson ,  Raj Rajagopal ,  Ramasubramani Kuduva Raman Thanumoorthy

发明人： Kurt J. Halverson ,  Raj Rajagopal ,  Ramasubramani Kuduva Raman Thanumoorthy

IPC分类号： G01N15/04

CPC分类号： G01N15/042 ,  B01L3/5021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12Q1/02 ,  G01N1/4077 ,  G01N15/04 ,  G01N21/07 ,  G01N2001/4083 ,  G01N2015/0065 ,  G01N2015/045 ,  G01N2333/245 ,  Y10T436/25375 ,  Y10T436/255

摘要： Method for detecting an analyte of interest in a sample. The method can include providing a container comprising a microstructured surface, and centrifuging the container toward the microstructured surface to form a sediment and a supernatant of the sample. Following centrifugation, the container can be inverted to decant at least a portion of the supernatant of the sample from the second portion, such that a concentrate (e.g., comprising the sediment) of the sample is retained in the microstructured surface. The concentrate can then be interrogated in the microstructured surface for the analyte of interest. In some embodiments, at least a portion of the second portion can be substantially transparent, such that the concentrate can be interrogated from the outside of the container, without requiring that the container be opened prior to interrogation.

摘要翻译： 用于检测样品中感兴趣的分析物的方法。 该方法可以包括提供包含微结构化表面的容器，并将容器离心到微结构化表面以形成样品的沉淀物和上清液。 离心后，将容器倒置以从第二部分倾析样品的上清液的至少一部分，使得样品的浓缩物（例如包含沉淀物）保留在微结构化表面中。 然后可以在微结构化表面中询问浓缩物用于感兴趣的分析物。 在一些实施例中，第二部分的至少一部分可以是基本上透明的，从而可以从容器的外部询问浓缩物，而不需要在询问之前打开容器。