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    Method and apparatus for making porous agarose beads
    2.
    发明授权
    Method and apparatus for making porous agarose beads 有权
    制备多孔琼脂糖珠的方法和设备

    公开(公告)号：US07678302B2

    公开(公告)日：2010-03-16

    申请号：US11520775

    申请日：2006-09-13

    申请人： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Nanying Bian ,  Brian Gagnon ,  Joaquin Umana ,  Neil P. Soice

    发明人： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Nanying Bian ,  Brian Gagnon ,  Joaquin Umana ,  Neil P. Soice

    IPC分类号： B29B9/00

    CPC分类号： B01J20/24 ,  B01J13/04 ,  B01J20/28009 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3244 ,  B01J20/3272 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/4825

    摘要： The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

    摘要翻译： 本发明涉及用于形成琼脂糖或核心琼脂糖珠的方法和装置。 该方法包括将琼脂糖溶解/凝胶化在合适的液体中，将其与疏水性液体混合以形成乳液，并将该乳液保持在等于或大于琼脂糖凝胶点的温度，将其通过静态混合器以产生 琼脂糖滴并在第二浴疏水性液体中固化琼脂糖滴。 然后可以将珠子洗涤并使用或进一步加工以使琼脂糖交联和/或在琼脂糖上添加各种功能。 用于固化琼脂糖滴的另一种方法是通过使用热交换器在离开静态混合器之后连续冷却流。 对于“核心”珠粒，使用类似的方法，除了核心，优选以珠粒形式，在其进入第一疏水性液体之前将其引入琼脂糖，使得琼脂糖在芯上形成涂层。 可以使用与琼脂糖珠（由该方法或另一方法制备）或核心琼脂糖（由该方法或另一方法制备）相似的方法将多层琼脂糖添加到现有珠粒上。 还公开了一种用于运行该过程的装置。

    Room temperature stable agarose solutions
    3.
    发明授权
    Room temperature stable agarose solutions 有权
    室温稳定的琼脂糖溶液

    公开(公告)号：US08552177B2

    公开(公告)日：2013-10-08

    申请号：US13221439

    申请日：2011-08-30

    申请人： Wilson Moya ,  Neil P. Soice

    发明人： Wilson Moya ,  Neil P. Soice

    IPC分类号： C08B37/02 ,  C08B37/00 ,  C07H1/00 ,  C07H3/00

    CPC分类号： C08J3/09 ,  C08J2305/00 ,  C08L5/02 ,  C08L5/12 ,  D06M11/13 ,  D06M11/155 ,  D06M11/38 ,  D06M15/03 ,  D06M23/10 ,  Y10T428/24149 ,  Y10T428/249953 ,  Y10T442/20

    摘要： A method for making functionalized porous crosslinked polysaccharide gel coated structures used as liquid chromatography media is provided. The method includes impregnating a porous substrate with a room temperature stable aqueous polysaccharide solution containing water, 0.1% to 20% of a polysaccharide, 18% to 54% of a gel-inhibiting agent to prevent the gel from re-gelling, and 0.001% to 10% of an anionic fluorosurfactant for optimum solution coatability onto the substrate, each concentration is by total weight of the aqueous solution. Next water is evaporated from the coating, followed by exposing the dehydrated coating to a gelling agent thereby forming a porous polysaccharide gel coated substrate. Next the gel coated substrate is exposed to a crosslinking agent forming a porous crosslinked polysaccharide gel coated substrate. Next, the gel coated substrate is functionalized by contacting the porous polysaccharide gel coated substrate with sodium 3-bromopropanesulfonate, thereby attaching sulfopropyl ligands to the gel coated substrate, resulting in a functionalized porous absorptive polysaccharide gel coated chromatography structure. When the functionalized gel coated chromatography structure is used as liquid chromatography media, and a protein (e.g. lysozyme) containing solution contacts the media, the sulfopropyl ligands attached to the gel coated chromatography structure will bond to the protein, thereby removing the protein from the protein containg solution.

    摘要翻译： 提供了用作液相色谱介质的功能化多孔交联多糖凝胶涂层结构的方法。 该方法包括用含有水，0.1％至20％的多糖，18％至54％的凝胶抑制剂的室温稳定的水性多糖溶液浸渍多孔基材以防止凝胶重新凝胶化，并且0.001％ 至10％的阴离子含氟表面活性剂，用于在基材上获得最佳的溶液涂布性，每种浓度均为水溶液的总重量。 接下来的水从涂层中蒸发，然后将脱水涂层暴露于胶凝剂，从而形成多孔多糖凝胶涂覆的基质。 接下来，将凝胶涂覆的基底暴露于形成多孔交联多糖凝胶涂覆的基底的交联剂。 接下来，通过使多孔多糖凝胶涂覆的基质与3-溴丙磺酸钠接触使凝胶涂覆的底物功能化，从而将磺丙基配体连接到凝胶涂覆的基质上，得到官能化的多孔吸收多糖凝胶涂层色谱结构。 当将功能化凝胶涂层色谱结构用作液相色谱介质，并且含有蛋白质（例如溶菌酶）的溶液与培养基接触时，附着在凝胶涂层色谱结构上的磺丙基配体将与蛋白质结合，从而从蛋白质中除去蛋白质 包含解决方案

    ROOM TEMPERATURE STABLE AGAROSE SOLUTIONS
    6.
    发明申请
    ROOM TEMPERATURE STABLE AGAROSE SOLUTIONS 有权
    室温稳定的AGAROSE解决方案

    公开(公告)号：US20110311762A1

    公开(公告)日：2011-12-22

    申请号：US13221439

    申请日：2011-08-30

    申请人： Wilson Moya ,  Neil P. Soice

    发明人： Wilson Moya ,  Neil P. Soice

    IPC分类号： B32B3/12 ,  B32B3/26 ,  B32B5/02 ,  B05D5/00 ,  B05D3/10

    CPC分类号： C08J3/09 ,  C08J2305/00 ,  C08L5/02 ,  C08L5/12 ,  D06M11/13 ,  D06M11/155 ,  D06M11/38 ,  D06M15/03 ,  D06M23/10 ,  Y10T428/24149 ,  Y10T428/249953 ,  Y10T442/20

    摘要： The present invention provides porous functionalized crosslinked polysaccharide hydrogel coated structures and methods for making the same. The methods include impregnating a porous substrate with a room temperature stable aqueous polysaccharide solution containing, water, a polysaccharide having a concentration in the aqueous solution from about 0.1% to about 20%, a gel-inhibiting agent having a concentration in the aqueous solution from about 18 to about 54%, and an anionic fluorosurfactant having a concentration in the aqueous solution from about 0.001% to about 10% by total weight of the aqueous solution. Followed by evaporating the water from the polysaccharide coating; and exposing the dehydrated polysaccharide coating to a gelling agent form a porous polysaccharide hydrogel coated substrate. The hydrogel coated substrate is exposed to a crosslinking agent to form a porous crosslinked polysaccharide hydrogel coated substrate, which is then functionalized by the attachment of a ligand.

    摘要翻译： 本发明提供多孔官能化交联多糖水凝胶涂层结构及其制备方法。 所述方法包括用室温稳定的水性多糖溶液浸渍多孔基材，所述水性多糖溶液含有水，浓度在约0.1％至约20％的水溶液中的浓度为约0.1％至约20％的浓度的凝胶抑制剂， 约18至约54％的阴离子含氟表面活性剂和在水溶液中的浓度的水溶液的总重量的约0.001％至约10％的阴离子含氟表面活性剂。 随后从多糖涂层蒸发水; 并将脱水的多糖涂层暴露于胶凝剂形成多孔多糖水凝胶涂覆的基质。 将水凝胶涂覆的基底暴露于交联剂以形成多孔交联的多糖水凝胶涂覆的底物，然后通过配体的附着将其官能化。

    Room temperature stable agarose solutions
    8.
    发明申请
    Room temperature stable agarose solutions 有权

    公开(公告)号：US20100196589A1

    公开(公告)日：2010-08-05

    申请号：US12661979

    申请日：2010-03-26

    申请人： Wilson Moya ,  Neil P. Soice

    发明人： Wilson Moya ,  Neil P. Soice

    IPC分类号： B05D5/00

    CPC分类号： C08J3/09 ,  C08J2305/00 ,  C08L5/02 ,  C08L5/12 ,  D06M11/13 ,  D06M11/155 ,  D06M11/38 ,  D06M15/03 ,  D06M23/10 ,  Y10T428/24149 ,  Y10T428/249953 ,  Y10T442/20

    摘要： Room temperature stable, non-gelling polysaccharide solutions such as agaroses, dextrans and cyclodextrans are made by the present invention. It has been found that by incorporating certain gel-inhibiting additives into an aqueous polysaccharide solution, the gel point is reduced or eliminated and the solution remains liquid at room temperature indefinitely. Additives that have been found to work include salts, such as lithium chloride and zinc chloride and bases, such as sodium hydroxide and lithium hydroxide. Mixtures of said salts and said bases can also be used with the same desired results. The composition of these solutions of the present idea can be further modified to include other additives, such as organic co-solvents or non-solvents, pH modifiers, surfactants or other polymers to customize the properties of the solution to improve the processability for the desired application and to form structures such as films, beads and coated porous substrates.