摘要:
An object of the present invention is to provide an immunoassay of PSA using an agglutination accelerator, which has an agglutination accelerating effect equal to or stronger than the known agglutination accelerator; hardly generates non-specific turbidity; and hardly generates salting out even in a solution with a high salt concentration. The present invention relates to an immunoassay of a prostate-specific antigen comprising performing an antigen-antibody reaction in the presence of a polymer having a monomer unit derived from a monomer represented by the following general formula [2]: (wherein R1-R3 are each independently a hydrogen atom or an alkyl group optionally having a hydroxyl group; R4 is an alkylene group; R5 is an alkylene group optionally having a substituent and optionally having an oxygen atom in a chain; R6 is a hydrogen atom or a methyl group, and X is an oxygen atom or a —NH— group), and a kit of reagent for an immunoassay comprising a reagent containing an agglutination accelerator for the immunoassay.
摘要翻译:本发明的目的是提供使用凝集促进剂的PSA的免疫测定法,凝集促进剂的凝集促进效果等于或大于已知的凝集促进剂; 几乎不产生非特异性浊度; 即使在盐浓度高的溶液中也难以产生盐析。 本发明涉及前列腺特异性抗原的免疫测定法,包括在具有来源于下述通式[2]表示的单体的单体单元的聚合物存在下进行抗原抗体反应:(其中R < 1〜3个O 3各自独立地为氢原子或任选具有羟基的烷基; R 4为亚烷基; R 5为 任选具有取代基且任选在链中具有氧原子的亚烷基; R 6是氢原子或甲基,X是氧原子或-NH - 组)和用于免疫测定的试剂盒,其包含含有用于免疫测定的凝集促进剂的试剂。
摘要:
The present invention relates to (1) A reagent for an immunoassay of a target substance existing in a free form and a bound form in a specimen, comprising a latex 1 which is immobilized with a monoclonal antibody 1 for the target substance, and a latex 2 which has a different mean particle size from the latex 1 and is immobilized with a monoclonal antibody 2 having a different recognition site for the target substance from the antibody 1, (2) An immunoassay method comprising reacting the target substance with the reagent of (1) and determining an amount of the substance based on the result of an agglutination reaction among the target substance, the latex1 and the latex2, and (3) A reagent kit comprising a reagent of (1) and a reagent containing an agglutination accelerator for an antigen-antibody reaction.
摘要:
An object of the present invention is to provide a method utilizing a carrier for measuring hyaluronic acid wherein deposition of carrier is less, a storage stability is good and an accuracy in measurement has same high degree as that performed by the conventional reagents, and a reagent kit for the same. Also, the present invention relates to “a method for measuring hyaluronic acid, comprising forming a hyaluronic acid/HABP complex by contacting hyaluronic acid in a sample with HABP, reacting said complex with an anti-HABP antibody-supported carrier, measuring optical change by agglutination product generated by said reaction and calculating the quantity of hyaluronic acid from the measured value” and “a reagent kit for the measurement of hyaluronic acid comprising a reagent comprising a hyaluronic acid binding protein and a reagent comprising an anti-hyaluronic acid binding protein antibody-supported carrier”.
摘要:
The present invention relates to (1) A reagent for an immunoassay of a target substance existing in a free form and a bound form in a specimen, comprising a latex 1 which is immobilized with a monoclonal antibody 1 for the target substance, and a latex 2 which has a different mean particle size from the latex 1 and is immobilized with a monoclonal antibody 2 having a different recognition site for the target substance from the antibody 1, (2) An immunoassay method comprising reacting the target substance with the reagent of (1) and determining an amount of the substance based on the result of an agglutination reaction among the target substance, the latex1 and the latex2, and (3) A reagent kit comprising a reagent of (1) and a reagent containing an agglutination accelerator for an antigen-antibody reaction.
摘要:
The present invention relates to (1) A reagent for an immunoassay of a target substance existing in a free form and a bound form in a specimen, comprising a latex 1 which is immobilized with a monoclonal antibody 1 for the target substance, and a latex 2 which has a different mean particle size from the latex 1 and is immobilized with a monoclonal antibody 2 having a different recognition site for the target substance from the antibody 1, (2) An immunoassay method comprising reacting the target substance with the reagent of (1) and determining an amount of the substance based on the result of an agglutination reaction among the target substance, the latex1 and the latex2, and (3) A reagent kit comprising a reagent of (1) and a reagent containing an agglutination accelerator for an antigen-antibody reaction.