公开(公告)号：US20110088122A1

公开(公告)日：2011-04-14

申请号：US12951807

申请日：2010-11-22

申请人： LÁSZLÓ MÁRTON ,  Mihaly Czako

发明人： LÁSZLÓ MÁRTON ,  Mihaly Czako

IPC分类号： A01H5/00 ,  C12N5/02

CPC分类号： A01H1/04 ,  A01H1/06 ,  A01H4/005 ,  C12N15/8205

摘要： The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

公开(公告)号：US08105835B2

公开(公告)日：2012-01-31

申请号：US12951757

申请日：2010-11-22

申请人： László Márton ,  Mihaly Czako

发明人： László Márton ,  Mihaly Czako

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： A01H1/04 ,  A01H1/06 ,  A01H4/005 ,  C12N15/8205

摘要： The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

摘要翻译： 本发明提供了一种微生物单子叶植物的方法，其包括：（a）在初级培养基上培养来自单子叶植物茎尖的组织外植体，其中所述外植体已经在低温下预处理，并且所述初级培养基包含生长素或生长素 和细胞分裂素，产生全能的胚胎发生细胞培养物; （b）用冷温度处理全能的胚胎细胞培养物; （c）通过在二级培养基上培养来维持全能的胚胎发生细胞培养物，从而产生和维持单子叶植物的全能胚胎发生细胞培养物; 和（d）将步骤（c）的胚胎发生细胞培养物转移到第三培养基中以继续繁殖并产生具有根和芽的小植物，从而微型化单子叶植物。 本文描述的微繁殖技术提供植物用于开发精英植物品系，植物修复和生物质生产等目的。

公开(公告)号：US08030073B2

公开(公告)日：2011-10-04

申请号：US12951807

申请日：2010-11-22

申请人： László Márton ,  Mihaly Czako

发明人： László Márton ,  Mihaly Czako

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： A01H1/04 ,  A01H1/06 ,  A01H4/005 ,  C12N15/8205

摘要： The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

公开(公告)号：US20110070629A1

公开(公告)日：2011-03-24

申请号：US12951757

申请日：2010-11-22

申请人： LÁSZLÓ MÁRTON ,  Mihaly Czako

发明人： LÁSZLÓ MÁRTON ,  Mihaly Czako

IPC分类号： C02F3/34 ,  C12N5/02 ,  C12N15/82 ,  A01H1/00 ,  B09C1/10

CPC分类号： A01H1/04 ,  A01H1/06 ,  A01H4/005 ,  C12N15/8205

摘要： The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

摘要翻译： 本发明提供了一种微生物单子叶植物的方法，其包括：（a）在初级培养基上培养来自单子叶植物茎尖的组织外植体，其中所述外植体已经在低温下预处理，并且所述初级培养基包含生长素或生长素 和细胞分裂素，产生全能的胚胎发生细胞培养物; （b）用冷温度处理全能的胚胎细胞培养物; （c）通过在二级培养基上培养来维持全能的胚胎发生细胞培养物，从而产生和维持单子叶植物的全能胚胎发生细胞培养物; 和（d）将步骤（c）的胚胎发生细胞培养物转移到第三培养基中以继续繁殖并产生具有根和芽的小植物，从而微型化单子叶植物。 本文描述的微繁殖技术提供植物用于开发精英植物品系，植物修复和生物质生产等目的。

公开(公告)号：US07863046B2

公开(公告)日：2011-01-04

申请号：US11800719

申请日：2007-05-07

申请人： László Márton ,  Mihaly Czako

发明人： László Márton ,  Mihaly Czako

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： A01H1/04 ,  A01H1/06 ,  A01H4/005 ,  C12N15/8205

摘要： The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

摘要翻译： 本发明提供了一种微生物单子叶植物的方法，其包括：（a）在初级培养基上培养来自单子叶植物茎尖的组织外植体，其中所述外植体已经在低温下预处理，并且所述初级培养基包含生长素或生长素 和细胞分裂素，产生全能的胚胎发生细胞培养物; （b）用冷温度处理全能的胚胎细胞培养物; （c）通过在二级培养基上培养来维持全能的胚胎发生细胞培养物，从而产生和维持单子叶植物的全能胚胎发生细胞培养物; 和（d）将步骤（c）的胚胎发生细胞培养物转移到第三培养基中以继续繁殖并产生具有根和芽的小植物，从而微型化单子叶植物。 本文描述的微繁殖技术提供植物用于开发精英植物品系，植物修复和生物质生产等目的。

公开(公告)号：US06821782B2

公开(公告)日：2004-11-23

申请号：US10068584

申请日：2002-02-05

申请人： Laszlo Marton ,  Mihaly Czako

发明人： Laszlo Marton ,  Mihaly Czako

IPC分类号： C12N500

CPC分类号： C12N15/8259 ,  A01H4/005 ,  A01H4/008 ,  B09C1/105 ,  C02F3/327 ,  C12N15/8201 ,  C12N15/8205 ,  Y02W10/18

摘要： A method is provided for generating sustained totipotent tissue cultures of a plant of the Class Monocotyledonae, and for micropropagating such plant in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons.

摘要翻译： 提供了一种用于产生单子叶植物的植物的持续全能组织培养物的方法，并且用于在体外微量繁殖这种植物，其中栽培不成熟的花序以产生适于持续维持和繁殖的全能组织。 可以在光照条件下诱导组织的绿化，多层培养可以通过微量化繁殖。 如果需要，外来基因可以被引入到组织中，并且转基因植物可以独立于季节用于野外的植物修复技术和植物存储器中。

公开(公告)号：US20080282424A1

公开(公告)日：2008-11-13

申请号：US11800719

申请日：2007-05-07

申请人： Laszlo Marton ,  Mihaly Czako

发明人： Laszlo Marton ,  Mihaly Czako

IPC分类号： C12N15/82 ,  C12N5/00

CPC分类号： A01H1/04 ,  A01H1/06 ,  A01H4/005 ,  C12N15/8205

摘要： The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

摘要翻译： 本发明提供了一种微生物单子叶植物的方法，其包括：（a）在初级培养基上培养来自单子叶植物茎尖的组织外植体，其中所述外植体已经在低温下预处理，并且所述初级培养基包含生长素或生长素 和细胞分裂素，产生全能的胚胎发生细胞培养物; （b）用冷温度处理全能的胚胎细胞培养物; （c）通过在二级培养基上培养来维持全能的胚胎发生细胞培养物，从而产生和维持单子叶植物的全能胚胎发生细胞培养物; 和（d）将步骤（c）的胚胎发生细胞培养物转移到第三培养基中以继续繁殖并产生具有根和芽的小植物，从而微型化单子叶植物。 本文描述的微繁殖技术提供植物用于开发精英植物品系，植物修复和生物质生产等目的。

公开(公告)号：US20050102719A1

公开(公告)日：2005-05-12

申请号：US10982254

申请日：2004-11-05

申请人： Laszlo Marton ,  Mihaly Czako

发明人： Laszlo Marton ,  Mihaly Czako

IPC分类号： A01H4/00 ,  B09C1/10 ,  C02F3/32 ,  C12N5/02 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C12N5/04

CPC分类号： C12N15/8259 ,  A01H4/005 ,  A01H4/008 ,  B09C1/105 ,  C02F3/327 ,  C12N15/8201 ,  C12N15/8205 ,  Y02W10/18

摘要： A method is provided for generating sustained totipotent tissue cultures of a plant of the Class Monocotyledonae, and for micropropagating such plant in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons.

摘要翻译： 提供了一种用于产生单子叶植物的植物的持续全能组织培养物的方法，并且用于在体外微量繁殖这种植物，其中栽培不成熟的花序以产生适于持续维持和繁殖的全能组织。 可以在光照下诱导组织的绿化，并且多中心培养物可以通过微量培养繁殖。 如果需要，外来基因可以被引入到组织中，并且转基因植物可以独立于季节用于野外的植物修复技术和植物存储器中。

公开(公告)号：US06087547A

公开(公告)日：2000-07-11

申请号：US318593

申请日：1999-05-25

申请人： Laszlo Marton ,  Yung-Pin Chen ,  Mihaly Czako

发明人： Laszlo Marton ,  Yung-Pin Chen ,  Mihaly Czako

IPC分类号： A62D3/02 ,  A62D3/00 ,  B09C1/10

CPC分类号： B09C1/105 ,  B09C1/10

摘要： It has been discovered that an enzyme produced by Spartina alterniflora degrades halogenated organics. The present invention is therefore the use of the enzyme produced by genes of this plant to remediate soils contaminated with halogenated organics such as TCE, and to genetically alter other plants to produce this enzyme using the gene that produces plant dehaloperoxidase.

摘要翻译： 已经发现由交替花粉生产的酶降解卤代有机物。 因此，本发明是使用由该植物的基因产生的酶来修复被卤代有机物如TCE污染的土壤，并且使用产生植物脱卤酶的基因遗传地改变其它植物以产生该酶。

公开(公告)号：US07303916B2

公开(公告)日：2007-12-04

申请号：US10982254

申请日：2004-11-05

申请人： Laszlo Marton ,  Mihaly Czako

发明人： Laszlo Marton ,  Mihaly Czako

IPC分类号： C12N5/02

CPC分类号： C12N15/8259 ,  A01H4/005 ,  A01H4/008 ,  B09C1/105 ,  C02F3/327 ,  C12N15/8201 ,  C12N15/8205 ,  Y02W10/18

摘要： A method is provided for generating sustained totipotent tissue cultures of a plant of the Class Monocotyledonae, and for micropropagating such plant in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons.

摘要翻译： 提供了一种用于产生单子叶植物的植物的持续全能组织培养物的方法，并且用于在体外微量繁殖这种植物，其中栽培不成熟的花序以产生适于持续维持和繁殖的全能组织。 可以在光照条件下诱导组织的绿化，多层培养可以通过微量化繁殖。 如果需要，外来基因可以被引入到组织中，并且转基因植物可以独立于季节用于野外的植物修复技术和植物存储器中。