公开(公告)号：US20160069808A1

公开(公告)日：2016-03-10

申请号：US14480258

申请日：2014-09-08

Applicant: LI-COR, Inc.

Inventor： Ahmed BOUZID ,  Chris LESIAK

IPC: G01N21/64 ,  G02B21/36 ,  G02B21/16 ,  G02B17/06 ,  G02B13/22

CPC classification number: G01N21/6458 ,  G01B11/0608 ,  G01N21/6456 ,  G01N2021/6463 ,  G01N2201/06113 ,  G01N2201/1053 ,  G02B13/22 ,  G02B17/0605 ,  G02B21/082 ,  G02B21/16 ,  G02B21/244 ,  G02B21/26 ,  G02B21/361 ,  G02B21/367

Abstract: Quantitative fluorescence imaging systems and methods using angular illumination to obtain automatic focus information. Laser scanning (e.g., point or line scanning) with angular illumination in combination with an area imaging sensor, such as with a bi-telcentric scanner, is used to determine sample height (relative to a detection axis orthogonal to a platform holding the sample) and also correct for sample height in subsequent scans.

Abstract translation: 使用角度照明的定量荧光成像系统和方法获得自动聚焦信息。 使用具有角照明的激光扫描（例如，点或线扫描）与区域成像传感器（例如使用双远心扫描器）结合来确定样品高度（相对于与保持样品的平台垂直的检测轴） 并在随后的扫描中校正样品高度。