公开(公告)号：US09758824B2

公开(公告)日：2017-09-12

申请号：US15231480

申请日：2016-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon R. Sauer ,  Bart J. Van Zeghbroeck

IPC: H01L29/66 ,  C12Q1/68 ,  G01N33/487 ,  G01N27/447 ,  B82Y30/00

CPC classification number: C12Q1/6869 ,  B82Y30/00 ,  C12Q1/6874 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand.

公开(公告)号：US09228976B2

公开(公告)日：2016-01-05

申请号：US14039142

申请日：2013-09-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon R. Sauer ,  Bart J. Van Zeghbroeck

IPC: H01L21/00 ,  G01N27/447 ,  C12Q1/68 ,  G01N33/487 ,  B82Y30/00

CPC classification number: C12Q1/6869 ,  B82Y30/00 ,  C12Q1/6874 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand.

Abstract translation: 使用至少一个半导体器件或绝缘和金属层的布置的系统和方法，其具有至少一个检测区域，该检测区域可以包括例如其中的凹部或开口，用于检测代表聚合物组分的电荷 ，例如靠近检测区域的核酸链，以及制造这种半导体器件的方法。 因此，该系统和方法可用于测序核糖核酸（RNA）或脱氧核糖核酸（DNA）的单个核苷酸或碱基。 半导体器件包括至少两个掺杂区域，例如注入在p型半导体层中的两个n型区域或者注入在n型半导体层中的两个p型区域。 响应于聚合物的组分（例如DNA或RNA链的碱基）的存在，检测区域允许电流在两个掺杂区域之间通过。 电流具有代表聚合物组分的特征，例如代表DNA或RNA链的检测碱基的特征。

公开(公告)号：US20150284790A1

公开(公告)日：2015-10-08

申请号：US14745753

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon R. Sauer ,  Bart J. Van Zeghbroeck

IPC: C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  B82Y30/00 ,  C12Q1/6874 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand.

Abstract translation: 使用至少一个半导体器件或绝缘和金属层的布置的系统和方法，其具有至少一个检测区域，该检测区域可以包括例如其中的凹部或开口，用于检测代表聚合物组分的电荷 ，例如靠近检测区域的核酸链，以及制造这种半导体器件的方法。 因此，该系统和方法可用于测序核糖核酸（RNA）或脱氧核糖核酸（DNA）的单个核苷酸或碱基。 半导体器件包括至少两个掺杂区域，例如注入在p型半导体层中的两个n型区域或者注入在n型半导体层中的两个p型区域。 响应于聚合物的组分（例如DNA或RNA链的碱基）的存在，检测区域允许电流在两个掺杂区域之间通过。 电流具有代表聚合物组分的特征，例如代表DNA或RNA链的检测碱基的特征。

公开(公告)号：US09410923B2

公开(公告)日：2016-08-09

申请号：US14745753

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon R. Sauer ,  Bart J. Van Zeghbroeck

IPC: H01L29/66 ,  G01N27/447 ,  C12Q1/68 ,  G01N33/487 ,  B82Y30/00

CPC classification number: C12Q1/6869 ,  B82Y30/00 ,  C12Q1/6874 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand.