公开(公告)号：US10246410B2

公开(公告)日：2019-04-02

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun Zhou ,  Kyle Gee ,  Yolanda Tennico ,  Peter Slade ,  Hee Chol Kang ,  Shaheer Khan ,  Brian Evans ,  James Stray

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US11535885B2

公开(公告)日：2022-12-27

申请号：US15733557

申请日：2019-03-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Khairuzzaman Bashar Mullah ,  Brian Evans ,  Scott C. Benson ,  Chu-An Chang ,  Xiongwei Yan

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

公开(公告)号：US20250092261A1

公开(公告)日：2025-03-20

申请号：US18580853

申请日：2022-07-21

Applicant: Life Technologies Corporation

Inventor： Andrew Levitz ,  Scott Benson ,  Brian Evans ,  Khairuzzaman Bashar Mullah

IPC: C09B57/14 ,  C12Q1/686 ,  G01N21/64

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described. Also disclosed is a method of detecting or quantifying a target nucleic acid molecule in a sample by polymerase chain reaction (PCR). The compounds have the following formula (I).

公开(公告)号：US11046727B2

公开(公告)日：2021-06-29

申请号：US15682103

申请日：2017-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tom Xu ,  Christopher Trinh ,  Carole Bornarth ,  Brian Evans ,  Mousumi Rath ,  Kathy Tran

IPC: C07H21/02 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q1/6809 ,  C12Q1/6834

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

公开(公告)号：US10793518B2

公开(公告)日：2020-10-06

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun Zhou ,  Kyle Gee ,  Yolanda Tennico ,  Peter Slade ,  Hee Chol Kang ,  Shaheer Khan ,  Brian Evans ,  James Stray

IPC: C07C309/47 ,  C07D311/84 ,  C07C309/51 ,  G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.