公开(公告)号：US10465237B2

公开(公告)日：2019-11-05

申请号：US15113030

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carmen Gjerstad ,  Shoulian Dong

IPC: C12Q1/68 ,  C12Q1/6851 ,  G16B20/00 ,  G16B25/20

Abstract: A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.

公开(公告)号：US20170022552A1

公开(公告)日：2017-01-26

申请号：US15113030

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carmen Gjerstad ,  Shoulian Dong

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  G16B20/00 ,  G16B25/20 ,  C12Q2523/125 ,  C12Q2537/165 ,  C12Q2545/113 ,  C12Q2545/114

Abstract: A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.

Abstract translation: 提供了一种用于定量生物样品中生物材料的方法。 该方法包括从第一和第二参考样品的扩增接收放大数据，并从生物样品的扩增接收扩增数据。 所述方法还包括根据来自第一和第二参考样本的放大的接收到的放大数据确定效率。 该方法包括确定生物样品的相对PCR效率。 接下来，该方法包括使用相对PCR效率确定生物样品中的生物材料的量。