公开(公告)号：US10137452B2

公开(公告)日：2018-11-27

申请号：US15410708

申请日：2017-01-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Conner ,  Chee Kiong Lim ,  Michael Pallas ,  Chee Wee Ching

IPC: B01L7/00 ,  C12M1/40 ,  C12Q3/00

Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.

公开(公告)号：US10471431B2

公开(公告)日：2019-11-12

申请号：US14613496

申请日：2015-02-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chee Kiong Lim ,  Way Xuang Lee ,  Wuh Ken Loh ,  Chee Wee Ching ,  Chin Yong Koo ,  Siew Ying Koh ,  Tiong Han Toh ,  Kuan Moon Boo ,  Soo Yong Lau

IPC: B01L7/00 ,  C12Q1/6806

Abstract: In one aspect, a thermal cycler system including a sample block and a thermoelectric device is disclosed. In various embodiments, the sample block has a first surface configured to receive a plurality of reaction vessels and an opposing second surface. In various embodiments the thermoelectric device is operably coupled to the second surface of the sample block. In various embodiments a thermal control unit is provided. In various embodiments the thermal control unit includes a computer processing unit. In various embodiments the thermal control unit includes an electrical current source. In various embodiments the thermal control unit also includes an electrical interface portion configured to connect the thermoelectric device with the electrical current source by way of an electrical cable. In various embodiments the thermal control unit is oriented in a different plane than the sample block and thermoelectric cooler.

公开(公告)号：US20170225169A1

公开(公告)日：2017-08-10

申请号：US15410708

申请日：2017-01-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Conner ,  Chee Kiong Lim ,  Michael Pallas ,  Chee Wee Ching

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2300/1894

Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.