-
公开(公告)号：US20240230501A1
公开(公告)日：2024-07-11
申请号：US18612831
申请日：2024-03-21
Applicant: Life Technologies Corporation
Inventor： Mark Andersen , Haopeng Wang , Michael Pallas
IPC: G01N15/04 , C12Q1/6816 , C12Q1/686
CPC classification number: G01N15/04 , C12Q1/6816 , C12Q1/686
Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
-
公开(公告)号：US10994275B2
公开(公告)日：2021-05-04
申请号：US16291757
申请日：2019-03-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael Pallas , Evan Foster , Jorge Fonseca , James Nurse , Theodore Straub
Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.
-
公开(公告)号：US20170225169A1
公开(公告)日：2017-08-10
申请号：US15410708
申请日：2017-01-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Thomas Conner , Chee Kiong Lim , Michael Pallas , Chee Wee Ching
IPC: B01L7/00
CPC classification number: B01L7/52 , B01L2200/147 , B01L2300/1822 , B01L2300/1894
Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.
-
Patent Agency Ranking
公开(公告)号：US20150044686A1
公开(公告)日：2015-02-12
申请号：US14385765
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael Pallas , James C. Nurse , Kevin Maher , Jorge Fonseca , Eliodor Ghenciu , Evan Foster
CPC classification number: B01L3/50857 , B01L3/50 , B01L3/50851 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2300/046 , B01L2300/0654 , B01L2300/0829 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , C12Q1/6806 , C12Q1/686 , G03F7/2002
Abstract: An article for holding a plurality of biological samples includes a substrate a substrate comprising a first surface and an opposing second surface and a plurality of reaction sites in the substrate. Each of the reaction sites extends from an opening in the first surface to an opening in the second surface. The reaction sites comprise a hexagonal shape and are configured to provide sufficient surface tension by capillary action to hold respective biological samples. The reaction sites have a density over at least a portion of the surfaces that is at least 170 holes per square millimeter. At least one of the surfaces may have a surface roughness characterized by an arithmetic average roughness (Ra) that is less than or equal to 5 nanometers.
Abstract translation: 用于保持多个生物样品的制品包括基材，其包含基材中的第一表面和相对的第二表面和多个反应位点。 每个反应位点从第一表面中的开口延伸到第二表面中的开口。 反应位点包括六边形形状并且被配置为通过毛细作用提供足够的表面张力以保持各自的生物样品。 反应部位的表面密度至少在每平方毫米170个孔以上。 至少一个表面可以具有由小于或等于5纳米的算术平均粗糙度（Ra）表征的表面粗糙度。
-
公开(公告)号：US11965809B2
公开(公告)日：2024-04-23
申请号：US17525477
申请日：2021-11-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Mark Andersen , Michael Pallas , Haopeng Wang
IPC: C12Q1/6816 , C12Q1/686 , G01N15/04
CPC classification number: G01N15/04 , C12Q1/6816 , C12Q1/686 , C12Q1/6816 , C12Q2563/107 , C12Q2563/159 , C12Q2565/607 , C12Q2565/629
Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
-
公开(公告)号：US11193872B2
公开(公告)日：2021-12-07
申请号：US16222664
申请日：2018-12-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Mark Andersen , Michael Pallas , Haopeng Wang
IPC: C12Q1/6816 , G01N15/04 , C12Q1/686
Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
-
公开(公告)号：US10252272B2
公开(公告)日：2019-04-09
申请号：US14893356
申请日：2014-05-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael Pallas , Evan Foster , Jorge Fonseca , James Nurse , Theodore Straub
Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.
-
公开(公告)号：US10137452B2
公开(公告)日：2018-11-27
申请号：US15410708
申请日：2017-01-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Thomas Conner , Chee Kiong Lim , Michael Pallas , Chee Wee Ching
Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.
-
-
-
-
-
-
-
Search Results
8
records
(took 0.03 seconds)
