公开(公告)号：US20180149847A1

公开(公告)日：2018-05-31

申请号：US15825895

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven LYTLE ,  Paul BOESCHOTEN ,  Andrew GUNDERSON ,  Chris GNEHM

IPC: G02B21/00 ,  G02B21/36

CPC classification number: G02B21/0032 ,  G02B21/0044 ,  G02B21/0056 ,  G02B21/0076 ,  G02B21/362 ,  G02B27/141

Abstract: An apparatus for generating structured illumination images, comprising a housing; imaging optics mounted within the housing, the imaging optics configured to focus illumination from an illumination source such that an in-focus pattern of light corresponding to a pattern on a photomask is projected onto a sample; a reflector mounted within the housing and configured to reflect the illumination to a sample; a filter apparatus, comprising one or more filters mounted within the housing, the filter apparatus configured to allow only emissions from the illuminated sample to pass through to the sensor; and mounting features on the housing, the mounting features configured to allow the apparatus to be installed within an imaging system.

公开(公告)号：US20180157022A1

公开(公告)日：2018-06-07

申请号：US15826125

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven LYTLE ,  Paul BOESCHOTEN ,  Andrew GUNDERSON ,  Larry RYSTROM ,  Chris GNEHM

IPC: G02B21/00 ,  G01N21/27

Abstract: A method for calibrating an imaging system, comprising: illuminating a sample through a pinhole mask using an excitation light; capturing an image of the sample using a sensor; converting the image into data; in a processing module: filtering the data using known spacing of pinholes in the pinhole mask to obtain data that corresponds to the spacing, using a threshold to identify regions of the remaining data that are bright enough to be associated with a pinhole, calculating the centroids of the regions, and fitting a known pattern for the pinhole mask to the regions in order to identify the best fit for the data; and storing, in a storage medium, the best fit data for use in a subsequent confocal capture routine.

公开(公告)号：US20180157021A1

公开(公告)日：2018-06-07

申请号：US15826021

申请日：2017-11-29

Applicant: Life Technologies Corporation

Inventor： Steven LYTLE ,  Paul BOESCHOTEN ,  Andrew GUNDERSON ,  Larry RYSTROM ,  Chris GNEHM

IPC: G02B21/00 ,  G02B21/36 ,  G01N21/64 ,  G03F7/20

CPC classification number: G02B21/0076 ,  G01N21/645 ,  G01N21/6458 ,  G01N2021/6463 ,  G01N2021/6478 ,  G02B21/367 ,  G03F7/2004

Abstract: A method for generating a composite image obtained in a fluorescence imaging system, comprising: determining the physical locations of a pinhole mask relative to a sample required to construct a full composite confocal image; generating in a control module a randomized order for the determined physical locations; moving the photomask or the sample to the determined physical locations in the randomized order under control of the control module and using a translation stage; illuminating the sample through the photomask using an excitation light; capturing a plurality of images at each of the physical locations using a sensor in order to generate a set of data points; using the randomized order to generate a composite image based on the set of data points and measuring the brightness of at least some of the data points; and adjusting the brightness of some of the set of data points.